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Genetic Insert: Hybrid Promoter : Plas_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Proper citation: RRID:Addgene_113595 Copy
Genetic Insert: Hybrid Promoter : Plux_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113594 Copy
Genetic Insert: Hybrid Promoter : Prhl_lac_tet_bD
Vector Backbone Description: Backbone Size:3886; Vector Backbone:pmb1, +ROP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29302024
Comments: Note: Plasmid contains a tet operator instead of a lac operator between rop and myc. This change is not known to affect plasmid function.
Proper citation: RRID:Addgene_113593 Copy
Species: E. coli
Genetic Insert: Chloramphenicol resistance gene
Vector Backbone Description: Vector Backbone:pCas; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29884781
Proper citation: RRID:Addgene_113633 Copy
Species: Homo sapiens
Genetic Insert: p97
Vector Backbone Description: Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113510 Copy
Genetic Insert: mKaxxte2.5
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30804405
Comments: One clones a fragment of DNA sequence at the MCS, then uses a targeted nuclease, such as Cas9 to trigger a double-strand break. This is then repaired by homologous recombination resulting in restoration of an intact mKate2.5 fluorescent protein (cloned from Addgene #54828). It can be used to test Cas9 gRNA efficiency, as it was in the paper, or HR-capacity in different cells.
Proper citation: RRID:Addgene_113630 Copy
Species: Homo sapiens
Genetic Insert: I3
Vector Backbone Description: Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30344098
Proper citation: RRID:Addgene_113515 Copy
Genetic Insert: sacB
Vector Backbone Description: Vector Backbone:pDAI-SceI; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25172289
Comments: Addgene NGS found K43E and T318P within the SacB translation compared to the best match NCBI reference sequence (ACF93714.1). The depositing laboratory confirms that these mutations should not adversely affect plasmid function
Proper citation: RRID:Addgene_113635 Copy
Vector Backbone Description: Backbone Size:6775; Vector Backbone:pMLBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Trimethoprim
Defining Citation: PMID:15925406
Proper citation: RRID:Addgene_113634 Copy
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113518 Copy
Species: Mus musculus
Genetic Insert: I-Ab beta Derp1-117
Vector Backbone Description: Backbone Size:3818; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding alpha chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes. The antigenic peptide sequence (residues 117-127 of Der p 1 allergen) is encoded as a fusion to the N-terminus of I-Ab beta, connected via a Gly-Ser linker. This fusion is encoded just downstream of the signal peptide, which should be cleaved during post-translational processing. Alternative antigenic peptides can be cloned in place of Der p 1 117-127 by exploiting the XmaI and SpeI restriction sites flanking this sequence.
Proper citation: RRID:Addgene_113639 Copy
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pMBP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29449511
Proper citation: RRID:Addgene_113517 Copy
Species: Mus musculus
Genetic Insert: I-Ab alpha BirA
Vector Backbone Description: Backbone Size:3808; Vector Backbone:pRMHa-3; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29956152
Comments: This construct, when co-expressed with the corresponding beta chain construct in Drosophila S2 cells and induced with copper sulfate, results in secreted expression of soluble peptide:MHCII complexes.
Proper citation: RRID:Addgene_113638 Copy
Genetic Insert: TBG promoter, Top tumor suppressor gRNAs
Vector Backbone Description: Vector Backbone:TBG-Vector (Addgene #109396); Vector Types:Mammalian Expression, Lentiviral, AAV, CRISPR, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29503867
Proper citation: RRID:Addgene_113585 Copy
Species: Synthetic
Genetic Insert: EJ7-GFP variant of EGFP
Vector Backbone Description: Vector Backbone:pCMV6-AC; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29950655
Proper citation: RRID:Addgene_113617 Copy
Genetic Insert: 6xPK tag
Vector Backbone Description: Backbone Marker:Thermo scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Golden gate donor vector for gene targeting in Bacillus subtilis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31147909
Comments: Donor vector for Golden gate assembly reactions that encodes for a C-terminal 6xPK tag (V5). Overhangs generated upon BsaI cleavage : AGCG (RtagN) and TAAG (RtagC).
(Gruber lab reference pSG1107)
Proper citation: RRID:Addgene_117127 Copy
Genetic Insert: 6xHA Tag
Vector Backbone Description: Backbone Marker:Thermo scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Golden gate donor vector for gene targeting in Bacillus subtilis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31147909
Comments: Donor vector for Golden gate assembly reactions that encodes for a C-terminal 6xHA tag. Overhangs generated upon BsaI cleavage : AGCG (RtagN) and TAAG (RtagC).
(Gruber lab reference pSG1106)
Addgene NGS results identified an IS1 element located between the CAP binding site and the lac UV5 promoter. This element will not affect plasmid function.
Proper citation: RRID:Addgene_117126 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtPIN1
Vector Backbone Description: Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_117088 Copy
Species: Bacillus subtilis
Genetic Insert: Erythromycin resistance cassette for Bacillus subtilis selection
Vector Backbone Description: Backbone Marker:Thermo scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Golden gate donor vector for gene targeting in Bacillus subtilis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31147909
Comments: Donor vector that encodes for an Erythromycin selection marker for Golden gate assembly reactions. Overhangs generated upon BsaI cleavage : AATA (Rup) and TCTA (Rdo).
(Gruber lab reference pSG632)
Proper citation: RRID:Addgene_117121 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtPIN2
Vector Backbone Description: Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Proper citation: RRID:Addgene_117089 Copy
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