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Species: Drosophila melanogaster
Genetic Insert: Xbp1
Vector Backbone Description: Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17170705
Comments: uas-xbp1-EGFP was made by subcloning EGFP after the EcoRV site of xbp1, which generated a chimeric construct with EGFP fused after the 252nd amino-acid residue of the xbp1-RA isoform.
Proper citation: RRID:Addgene_80627 Copy
Species: Drosophila melanogaster
Genetic Insert: Wingless
Vector Backbone Description: Backbone Size:12500; Vector Backbone:pCaSpeR4; Vector Types:Drosophila Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18430724
Proper citation: RRID:Addgene_64321 Copy
Species: Drosophila melanogaster
Genetic Insert: CG13188
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To use this plasmid for S2 cell transfection, a short Kozac sequence was added 5' to the ATG of CG13188 : ccGCG-ATG...
Mutation H452N does not affect plasmid function.
Proper citation: RRID:Addgene_66791 Copy
Species: Drosophila melanogaster
Genetic Insert: ATG1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23819996
Proper citation: RRID:Addgene_66918 Copy
Species: Drosophila melanogaster
Genetic Insert: CPD photolyase
Vector Backbone Description: Backbone Marker:Gibco BRL; Backbone Size:6700; Vector Backbone:pFastbac, modified; Vector Types:bacterial vector, insert not expressed; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22175817
Comments: Construct is intermediate in making insect cell virus for expression. *Mutation was found during quality control and is not known to confer any functional change.
Proper citation: RRID:Addgene_67285 Copy
Species: Drosophila melanogaster
Genetic Insert: 6/4 photolyase from Drosophila
Vector Backbone Description: Backbone Marker:Gibco BRL; Backbone Size:5000; Vector Backbone:pFastbac; Vector Types:Intermediate for insect expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22175817
Proper citation: RRID:Addgene_67284 Copy
Species: Drosophila melanogaster
Genetic Insert: crumbs
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: The plasmid contains part of the 5' cDNA of Crb and the 3' part that brings the transmembrane and the intracellular domains of the protein together. The BglII restriction site links the 5' and 3' regions of the cDNA, meaning that the majority of the extracellular part of the Crb protein is missing. The fragment also contains the 5' and 3' UTR regions. BglII restriction site was used to clone a GFP fragment. GFP is inserted between aa 96 and 2122 of crb. Crb contains K89E mutation which does not affect plasmid function.
Proper citation: RRID:Addgene_67525 Copy
Species: Drosophila melanogaster
Genetic Insert: dyl - chitin binding domain - tdTomato - dyl
Vector Backbone Description: Backbone Marker:Norbert Perrimon; Backbone Size:7541; Vector Backbone:pWalium-moe; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26395478
Comments: The plasmid contains pieces of DNA from a number of different sources. The td-Tomato was obtained from FUtdTW, Addgene.
Chitin binding domain was purchased from New England Biolabs
The dyl cDNA came from the Drosophila genome project
The pWalium10 moe vector came originally from the N. Perrimon Lab.
Proper citation: RRID:Addgene_67756 Copy
Species: Drosophila melanogaster
Genetic Insert: Thisbe (FGF8like-1)
Vector Backbone Description: Backbone Marker:?; Backbone Size:8900; Vector Backbone:pUAST-HA; Vector Types:Insect Expression, P element-based pUAST vector for Gal4-regulated expression in Drosophila.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19515694
Proper citation: RRID:Addgene_69769 Copy
Species: Drosophila melanogaster
Genetic Insert: Laccase2
Vector Backbone Description: Backbone Size:7143; Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69884 Copy
Species: Drosophila melanogaster
Genetic Insert: Laccase2
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:5428; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69893 Copy
Species: Drosophila melanogaster
Genetic Insert: GFP, laccase2
Vector Backbone Description: Backbone Size:7143; Vector Backbone:Hy_pMT; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26450910
Proper citation: RRID:Addgene_69891 Copy
Species: Drosophila melanogaster
Genetic Insert: Pebble DH +PH domains
Vector Backbone Description: Backbone Marker:?; Backbone Size:8900; Vector Backbone:pUAST-HA; Vector Types:Insect Expression, P element-based pUAST vector for Gal4-regulated expression in Drosophila.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19176590
Proper citation: RRID:Addgene_69793 Copy
Species: Drosophila melanogaster
Genetic Insert: Pebble - Drosophila guanine nucleotide exchange factor
Vector Backbone Description: Backbone Marker:?; Backbone Size:8900; Vector Backbone:pUAST-HA; Vector Types:Insect Expression, P element-based pUAST vector for Gal4-regulated expression in Drosophila.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19176590
Proper citation: RRID:Addgene_69792 Copy
Species: Drosophila melanogaster
Genetic Insert: Pebble C-Term domain (Drosophila guanine nucleotide exchange factor)
Vector Backbone Description: Backbone Marker:?; Backbone Size:8900; Vector Backbone:pUAST-HA; Vector Types:Insect Expression, P element-based pUAST vector for Gal4-regulated expression in Drosophila.; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19176590
Proper citation: RRID:Addgene_69795 Copy
Species: Drosophila melanogaster
Genetic Insert: Rac1
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASp; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: Please note that the ORF contains a SNP at K186S.
Proper citation: RRID:Addgene_52234 Copy
Species: Drosophila melanogaster
Genetic Insert: Rac1
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASp; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: Please note that the ORF contains a SNP at K186S.
Proper citation: RRID:Addgene_52235 Copy
Species: Drosophila melanogaster
Genetic Insert: Rac1
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASp; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Comments: Please note that the ORF contains a SNP at K186S.
Proper citation: RRID:Addgene_52233 Copy
Species: Drosophila melanogaster
Genetic Insert: Rho1
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASp; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52231 Copy
Species: Drosophila melanogaster
Genetic Insert: Rac2
Vector Backbone Description: Backbone Size:9939; Vector Backbone:pUASp; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_52238 Copy
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