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Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: New version of Neomycin resistance vector for drug selection in worms.
This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells).
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34919 Copy
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290182
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms.
This vector contains a Pmyo-2::EGFP::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN40-R4R3 which has a red fluorescent pharyngeal marker.
This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_34916 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi14024 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34862 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.
Proper citation: RRID:Addgene_34861 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34864 Copy
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:12020; Vector Backbone:pBID-UASC-MRG; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Nterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
Proper citation: RRID:Addgene_35205 Copy
Species: Danio rerio
Genetic Insert: 4xU6:sgRNA
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227778 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 codon optimized for human
Vector Backbone Description: Vector Backbone:pX330_42230; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39721357
Comments: Expression vector from Cong et al. 10.1126/science.1231143 that carries the chloramphenicol resistance protein ABO28845.1 and the negative selection marker ccdB ABO28846.1 for efficient cloning of sgRNA targeting sequences.
Proper citation: RRID:Addgene_223321 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222042 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222040 Copy
Vector Backbone Description: Vector Backbone:pGGD000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222067 Copy
Vector Backbone Description: Vector Backbone:pGGC000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222066 Copy
Vector Backbone Description: Vector Backbone:pGGF000; Vector Types:Synthetic Biology, GreenGate compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39292669
Proper citation: RRID:Addgene_222069 Copy
Vector Backbone Description: Backbone Marker:Alexander Stark (Addgene # 99292); Vector Backbone:hSTARR-seq_SCP1 vector; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39716078
Proper citation: RRID:Addgene_220289 Copy
Vector Backbone Description: Vector Backbone:plix403; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223955 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223953 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223951 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223952 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223950 Copy
Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223948 Copy
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