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Species: Mus musculus
Genetic Insert: miR-302b, c, a, d
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454525
Proper citation: RRID:Addgene_31711 Copy
Species: Mus musculus
Genetic Insert: miR-19b
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454525
Proper citation: RRID:Addgene_31710 Copy
Species: Mus musculus
Genetic Insert: miR-92a
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454525
Proper citation: RRID:Addgene_31708 Copy
Species: Mus musculus
Genetic Insert: miR-106a-363
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454525
Comments: miR-106a-363 micro RNA sequence - caaagtgctaacagtgcaggtag
Proper citation: RRID:Addgene_31703 Copy
Species: Mus musculus
Genetic Insert: miR-18b
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454525
Proper citation: RRID:Addgene_31706 Copy
Species: Mus musculus
Genetic Insert: NLS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4678; Vector Backbone:N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20212155
Proper citation: RRID:Addgene_31871 Copy
Species: Mus musculus
Genetic Insert: nMyc wild-type 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20054295
Proper citation: RRID:Addgene_31858 Copy
Species: Mus musculus
Genetic Insert: nMyc mutant-1 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:psiCHECK-2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20054295
Proper citation: RRID:Addgene_31859 Copy
Species: Mus musculus
Genetic Insert: Akt1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21147110
Proper citation: RRID:Addgene_31790 Copy
Species: Mus musculus
Genetic Insert: TAZ
Vector Backbone Description: Backbone Marker:Drs. S Lessnick and T. Golub (Dana-Farber Cancer Institute); Vector Backbone:pSRP; Vector Types:Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16099986
Proper citation: RRID:Addgene_31795 Copy
Species: Mus musculus
Genetic Insert: MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7565789
Comments: The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The wild-type RSRF sequence of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.
Proper citation: RRID:Addgene_31818 Copy
Species: Mus musculus
Genetic Insert: Erk5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11046135
Proper citation: RRID:Addgene_31817 Copy
Species: Mus musculus
Genetic Insert: Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7565789
Comments: The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The mutated RSRF sequence (two CTATATTTAG RSRF sites mutated to CGATATTTCG) of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.
Proper citation: RRID:Addgene_31819 Copy
Species: Mus musculus
Genetic Insert: miR-9/9* and miR-124
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11315; Vector Backbone:pLemir; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21753754
Proper citation: RRID:Addgene_31779 Copy
Species: Mus musculus
Genetic Insert: ULK1
Vector Backbone Description: Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19225151
Proper citation: RRID:Addgene_31962 Copy
Species: Mus musculus
Genetic Insert: Metallothionein
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17692533
Comments: The metallothionein (MT) gene was amplified with the forward primer (5'-CTCGGGATCGAGGGAAGGATTTCAAGA TATACCATGGACCCC-3'), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (5'-GACT CTAGAGGATCCTAGGCACAGCACGTG-3' ), which contains the MT gene stop codon and a subsequent BamHI site. Both the PCR product and pMAL-c2x vector were digested with XmnI and BamHI and were later ligated together to generate the final plasmid. In addition to this plasmid Addgene offers versions with 2 copies (32102), 3 copies (32115) and 4 copies (32100) of MT
Proper citation: RRID:Addgene_32101 Copy
Species: Mus musculus
Genetic Insert: shRNA against mouse HDAC5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2854; Vector Backbone:pENTR/U6; Vector Types:RNAi, Gateway U6 ENTRY Vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21565617
Proper citation: RRID:Addgene_32222 Copy
Species: Mus musculus
Genetic Insert: shRNA targetting Hmga2
Vector Backbone Description: Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21471965
Proper citation: RRID:Addgene_32399 Copy
Species: Mus musculus
Genetic Insert: Rb promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20100864
Proper citation: RRID:Addgene_32390 Copy
Species: Mus musculus
Genetic Insert: p107 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20100864
Proper citation: RRID:Addgene_32392 Copy
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