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Species: E. coli
Genetic Insert: dnaN (beta), N-terminal his-kinase cleavable tag
Vector Backbone Description: Vector Backbone:pET-16b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The PKA kinase site and PreScission Protesase clevage site are added between the his-10 tag & Factor Xa sequence in pET(16b) and the start of the Ec dnaN ORF.
Proper citation: RRID:Addgene_237239 Copy
Species: E. coli
Genetic Insert: Minimal CRISPR polymerase
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:41034576
Comments: Please visit https://doi.org/10.1101/2025.03.28.646030 for the bioRxiv preprint.
Proper citation: RRID:Addgene_246064 Copy
Species: E. coli
Genetic Insert: Anti CBASS 2
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41034576
Comments: Please visit https://doi.org/10.1101/2025.03.28.646030 for the bioRxiv preprint.
Proper citation: RRID:Addgene_246062 Copy
Species: E. coli
Genetic Insert: Anti CBASS 1
Vector Backbone Description: Vector Backbone:SC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41034576
Comments: Please visit https://doi.org/10.1101/2025.03.28.646030 for the bioRxiv preprint.
Proper citation: RRID:Addgene_246067 Copy
Species: E. coli
Genetic Insert: Minimal CRISPR polymerase
Vector Backbone Description: Vector Backbone:pET18; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41034576
Comments: Please visit https://doi.org/10.1101/2025.03.28.646030 for the bioRxiv preprint.
Proper citation: RRID:Addgene_246071 Copy
Species: E. coli
Genetic Insert: NusG
Vector Backbone Description: Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633055
Proper citation: RRID:Addgene_242076 Copy
Species: E. coli
Genetic Insert: rpsa
Vector Backbone Description: Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633055
Proper citation: RRID:Addgene_242079 Copy
Species: E. coli
Genetic Insert: metG
Vector Backbone Description: Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633055
Proper citation: RRID:Addgene_242077 Copy
Species: E. coli
Genetic Insert: NusG
Vector Backbone Description: Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633055
Proper citation: RRID:Addgene_242080 Copy
Species: E. coli
Genetic Insert: NusG
Vector Backbone Description: Vector Backbone:pESUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633055
Proper citation: RRID:Addgene_242081 Copy
Species: E. coli
Genetic Insert: btuB
Vector Backbone Description: Backbone Marker:Yale Coli Genetic Stock Center; Backbone Size:6236; Vector Backbone:pBAD42; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29298049
Proper citation: RRID:Addgene_237541 Copy
Species: E. coli
Genetic Insert: rpoA-rpoB-Nter-ybbR-rpoC-6xHis and rpoZ
Vector Backbone Description: Vector Backbone:pVS10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39633055
Proper citation: RRID:Addgene_242082 Copy
Species: E. coli
Genetic Insert: Rnr S1 + basic domain (residues 1930 to 2442)
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_232580 Copy
Species: E. coli
Genetic Insert: Rnr nuclease domain (residues 649 to 1929)
Vector Backbone Description: Backbone Size:4969; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_232579 Copy
Species: E. coli
Genetic Insert: GST-YihI with N+C-term lysines (K) mutated to arginine (R)
Vector Backbone Description: Backbone Size:4958; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_233093 Copy
Species: E. coli
Genetic Insert: GST-YihI with all lysines (K) mutated to arginine (R)
Vector Backbone Description: Backbone Size:4958; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_233094 Copy
Species: E. coli
Genetic Insert: YihI
Vector Backbone Description: Backbone Size:4958; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40621900
Comments: Please visit https://doi.org/10.1101/2025.02.12.637445 for bioRxiv preprint.
Proper citation: RRID:Addgene_233088 Copy
Species: E. coli
Genetic Insert: NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5409; Vector Backbone:pET21a (+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40346061
Proper citation: RRID:Addgene_215437 Copy
Species: E. coli
Genetic Insert: PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E)
Vector Backbone Description: Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238344 Copy
Species: E. coli
Genetic Insert: PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A)
Vector Backbone Description: Vector Backbone:pRSFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_238343 Copy
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