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Genetic Insert: tdTomato/amiR-eGFP123/amiR-eGFP419/FLPe
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26285215
Proper citation: RRID:Addgene_62710 Copy
Species: Stigeoclonium helveticum
Genetic Insert: Chronos-tdTomato
Vector Backbone Description: Backbone Marker:original from Stratagene; Backbone Size:4368; Vector Backbone:AAV-Syn; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24509633
Comments: The plasmid is fully sequenced in the coding sequence regions (opsin-fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
Proper citation: RRID:Addgene_62726 Copy
Species: Streptococcus pyogenes serotype M1
Genetic Insert: SP-CAS9
Vector Backbone Description: Backbone Size:5562; Vector Backbone:pET-15; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25910214
Proper citation: RRID:Addgene_62731 Copy
Species: Synthetic
Genetic Insert: CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
Vector Backbone Description: Backbone Size:8172; Vector Backbone:PHAGE2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25910214
Proper citation: RRID:Addgene_62733 Copy
Species: Homo sapiens
Genetic Insert: CACNA1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296916
Comments: contains alternate exons (+e10a, +18a, Δ19a, +e31a, +e37b and +e46)
Note that the human CaV2.2 insert contains the native stop codon. The V5 and His tags present in the pcDNA6/V5-HisA backbone are NOT in frame with CaV2.2.
Proper citation: RRID:Addgene_62574 Copy
Genetic Insert: MCS with F2A and T2A
Vector Backbone Description: Backbone Marker:Agilent; Vector Backbone:pShuttle-CMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62621 Copy
Species: Homo sapiens
Genetic Insert: hAlpha7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62629 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62638 Copy
Species: E. coli
Genetic Insert: Thr251Gly-EcPheRS
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4541; Vector Backbone:pQE-80L-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25691744
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_62598 Copy
Genetic Insert: exo, beta, gam, sgRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26463009
Proper citation: RRID:Addgene_62654 Copy
Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26463009
Comments: Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected.
Proper citation: RRID:Addgene_62655 Copy
Genetic Insert: exo, beta, gam, sgRNA
Vector Backbone Description: Vector Backbone:pKD46; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:26463009
Proper citation: RRID:Addgene_62656 Copy
Species: Synthetic
Genetic Insert: mtZFN(-)2G
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24567072
Proper citation: RRID:Addgene_62799 Copy
Species: Homo sapiens
Genetic Insert: CenpA
Vector Backbone Description: Vector Backbone:N1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62742 Copy
Species: Mus musculus
Genetic Insert: Talin
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62763 Copy
Species: Homo sapiens
Genetic Insert: PML-IV
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205865
Comments: The PML isoform in this plasmid is referred to as PML-IV according to the nomenclature established by Jensen, Shiels and Freemont Oncogene. 2001 Oct 29;20(49):7223-33. The GenBank nomenclature for PML-IV is PML isoform 6 (GenBank ID NM_002675.3).
Proper citation: RRID:Addgene_62804 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pFG001; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_62816 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pFG018; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_62817 Copy
Genetic Insert: T2A-QF2-Hsp70
Vector Backbone Description: Backbone Marker:Hugo Bellen; Backbone Size:3394; Vector Backbone:pBS-KS-attB1-2-PT-SA-SD-0; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732830
Comments: This plasmid was generated by the laboratory of Christopher Potter and is based on the QF2 construct (http://www.addgene.org/46115/) published at: http://dx.doi.org/10.1038/nmeth.3250
Other reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.
Proper citation: RRID:Addgene_62944 Copy
Genetic Insert: T2A-LexA::QFAD-Hsp70
Vector Backbone Description: Backbone Marker:Hugo Bellen; Backbone Size:3394; Vector Backbone:pBS-KS-attB1-2-PT-SA-SD-0; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25732830
Comments: This plasmid was generated by the laboratory of Christopher Potter and is based on the LexA::QFAD construct (http://www.addgene.org/46123/) published at: http://dx.doi.org/10.1038/nmeth.3250
Other reference:
Venken KJT, et al, 2011, MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. Nature Method: 8:737-744.
Proper citation: RRID:Addgene_62947 Copy
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