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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEB2_mScarletI_mVenusNB-MrkHR113A Resource Report Resource Website |
RRID:Addgene_182292 | mVenusNB-MrkHR113A | Synthetic | Kanamycin | PMID:35131853 | We obtained the codon optimized MrkH sequence from Maria Schumacher, as described in her publication PMID: 27551088 | Backbone Marker:J. H. Davis, A. J. Rubin, R. T. Sauer, Design, construction and characterization of a set of insulated bacterial promoters.; Backbone Size:3200; Vector Backbone:pEB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | changed arginine 113 to alanine, MrkH numbering as in PMID: 27551088 | 2026-08-15 01:11:31 | 0 |
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pCAG-ArcLight-ST Resource Report Resource Website |
RRID:Addgene_182318 | ArcLight-ST | Synthetic | Ampicillin | PMID:34893595 | Backbone Size:4831; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Mutation in an amino acid Q217E of ArcLight-MT | 2026-08-15 01:11:31 | 0 | |
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pCDNA3_cAMPFIRE-L Resource Report Resource Website 1+ mentions |
RRID:Addgene_182279 | cAMPFIRE-L (cAMP sensor) | Synthetic | Ampicillin | PMID:36303019 | Please visit https://doi.org/10.1101/2021.08.27.457999 for bioRxiv preprint. | Backbone Size:5428; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:31 | 1 | |
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pCAG-Kv-ArcLight-ST Resource Report Resource Website |
RRID:Addgene_182319 | Kv-ArcLight-ST | Synthetic | Ampicillin | PMID:34893595 | Backbone Size:4794; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Mutation in an amino acid Q217E of ArcLight-MT | 2026-08-15 01:11:31 | 0 | |
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pCMV GFP1-9 Resource Report Resource Website 1+ mentions |
RRID:Addgene_182244 | GFP 1-9 OPT | Synthetic | Kanamycin | PMID:24092409 | Backbone Marker:pEGFP (Clontech); Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:11:31 | 2 | ||
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mT2A-tdTomato-FNF Resource Report Resource Website |
RRID:Addgene_182340 | T2A-tdTomato-FNF | Synthetic | Ampicillin | Release the insert with PmeI and PacI digest. | Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:31 | 0 | ||
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mT2A-TagRFP-T-linker-dre-PBD*-FNF Resource Report Resource Website |
RRID:Addgene_182342 | T2A-TagRFP-T-linker-dre-PBD*-FNF | Synthetic | Ampicillin | Release the insert with PmeI and PacI digest. | Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:32 | 0 | ||
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pMOD4-mT2A-mutated_rpsL-BSD-F Resource Report Resource Website |
RRID:Addgene_182338 | BSD | Synthetic | Ampicillin | PCR amplify around the 5' T2A and 3' FRT site, making sure to add a PacI site at the 3' of the FRT site. As suggested in the plasmid name, due to a PCR error, the rpsL open reading frame has a 1-bp deletion that results in a frame shift and as a consequence premature stop codons. Despite this, the downstream BSD open reading frame is translated from the bicistronic RNA in E. coli without problems. These mean that the mutated rpsL cannot be used for streptomycin counterselection, but the unmutated BSD can and was successfully used several times to confer blasticidin resistance for selection after recombineering. | Backbone Marker:Alfred L. Fisher; Vector Backbone:pMOD4-galK-G; Vector Types:Bacterial Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:31 | 0 | ||
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mT2A-td-sfGFP-FNF Resource Report Resource Website |
RRID:Addgene_182339 | T2A-td-sfGFP-FNF | Synthetic | Ampicillin | Release the insert with PmeI and PacI digest. | Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:31 | 0 | ||
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miR-124 Resource Report Resource Website |
RRID:Addgene_182363 | 4x miR-124 target sites | Synthetic | Ampicillin | Four copies of miR-124 target sites (Åkerblom et al., 2012, https://doi.org/10.1523/JNEUROSCI.0558-12.2012) were cloned into “StLa” reporter plasmid (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA from Nam and Benezra, 2009, https://doi.org/10.1016/j.stem.2009.08.017) without the tauLacZ insert and the Rosa26 homology arms. The 4x miR-124 target sequence can be released with BamHI and NotI. More information on the “StLa” reporter at (1) http://www.informatics.jax.org/allele/MGI:4366911 (2) https://figshare.com/ndownloader/files/38884434 (clicking this link will download a file) | Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:32 | 0 | ||
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loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox Resource Report Resource Website |
RRID:Addgene_182360 | Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2 | Synthetic | Ampicillin | Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox, Synthetic Biology, dre / rox , FLP / FRT; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:32 | 0 | |||
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ePPE-spG Resource Report Resource Website |
RRID:Addgene_183096 | Nls-nspGCas9-NC-nls-MLV-Nls | Synthetic | Ampicillin | PMID:35332341 | Vector Backbone:pJIT163; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin | D10A in SpGCas9 | 2026-08-15 01:11:34 | 0 | |
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pKT2/CAGXSP/PalmReNL Resource Report Resource Website 1+ mentions |
RRID:Addgene_182970 | PalmReNL | Synthetic | Kanamycin | PMID:36619349 | Backbone Marker:Michael H. Bachmann; Vector Backbone:pKT2/CAGXSP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | N/A | 2026-08-15 01:11:33 | 1 | |
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pCRISPRi-D2 Resource Report Resource Website |
RRID:Addgene_182927 | ddcpf1 | Synthetic | Kanamycin | PMID:32379958 | Vector Backbone:pRSF1010; Vector Types:CRISPR; Bacterial Resistance:Kanamycin | 2026-08-15 01:11:33 | 0 | ||
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SnFR_S074 Resource Report Resource Website |
RRID:Addgene_182811 | S074-CholineSnFR | Synthetic | Ampicillin | For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648. | Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:33 | 0 | ||
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SnFR_S062 Resource Report Resource Website |
RRID:Addgene_182810 | S062-CholineSnFR | Synthetic | Ampicillin | For additional reference, please see Nichols et al. 2022 Elife, doi.org/10.7554/eLife.74648. | Backbone Marker:Caltech; Backbone Size:2761; Vector Backbone:pHHM.X51; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:33 | 0 | ||
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kiCAP-AAV-BI30 Resource Report Resource Website 1+ mentions |
RRID:Addgene_183749 | AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1 | Synthetic | Kanamycin | PMID:35571675 | Backbone Size:3000; Vector Backbone:pUC57-Kan; Vector Types:AAV; Bacterial Resistance:Kanamycin | K449R, and NNSTRGG inserted between 588 and 589 of AAV9 VP1 | 2026-08-15 01:11:34 | 5 | |
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pSM-GFPnovo2 Resource Report Resource Website |
RRID:Addgene_116943 | C. elegans codon optimized GFPnovo2 | Synthetic | Ampicillin | PMID:32550394 | Please note that some discrepancies were found between Addgene's quality control and the depositor's full plasmid sequence. The depositor noted that the backbone was not sequenced, and these discrepancies do NOT affect plasmid function. GFPnovo2 was originally generated by Arakawa et al., (2008) Arakawa, H., Kudo, H., Batrak, V., Caldwell, R. B., Rieger, M. A., Ellwart, J. W., & Buerstedde, J.-M. (2008). Protein evolution by hypermutation and selection in the B cell line DT40. Nucleic Acids Research, 36(1), e1. http://doi.org/10.1093/nar/gkm616 | Backbone Marker:Cori Bargmann's lab; Backbone Size:3600; Vector Backbone:pSM; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | Y145F, V163A, S202T, L221V | 2026-08-15 01:02:49 | 0 |
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pTW051d2 Resource Report Resource Website |
RRID:Addgene_116893 | g3c | Synthetic | Spectinomycin and Streptomycin | PMID:30127387 | Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:02:48 | 0 | ||
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pTW051b4 Resource Report Resource Website |
RRID:Addgene_116891 | g3c | Synthetic | Spectinomycin and Streptomycin | PMID:30127387 | Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:02:49 | 0 |
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