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Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7251; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27211859
Proper citation: RRID:Addgene_90388 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9 guide scaffold with AtU626 promoter/terminator
Vector Backbone Description: Backbone Size:4372; Vector Backbone:n/a; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995631
Comments: User inserts own guide. Level 1 position 1
Proper citation: RRID:Addgene_191769 Copy
Species: Streptococcus pyogenes
Genetic Insert: SpCas9
Vector Backbone Description: Backbone Size:4897; Vector Backbone:n/a; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36995630
Comments: User inserts own guides for CRISPR in Medicago truncatula
Proper citation: RRID:Addgene_191768 Copy
Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7251; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27211859
Proper citation: RRID:Addgene_90385 Copy
Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7251; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27211859
Proper citation: RRID:Addgene_90384 Copy
Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7251; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27211859
Proper citation: RRID:Addgene_90380 Copy
Species: P. pyralis
Genetic Insert: Firefly luciferase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:7251; Vector Backbone:pLenti7.3; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27211859
Proper citation: RRID:Addgene_90383 Copy
Species: Mus musculus
Genetic Insert: tdTomato-T2A-mSyp::EGFP
Vector Backbone Description: Backbone Marker:Connie Cepko (Addgene plasmid # 13769); Backbone Size:6107; Vector Backbone:pCALNL; Vector Types:Mammalian Expression, pCAGEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36386881
Comments: Suggested primers to sequence the insert:
5' ccttcttgacgagttcttctg 3'
5' acgtctccgcatgtcagaag 3'
5' aaggcctgtccgatgtgaag 3'
5' ttcaggaagccaaacaccac 3'
5' GCTTGCCGTAGGTGGCATC 3'
5' CAGGAAACAGCTATGAC 3'
Proper citation: RRID:Addgene_191213 Copy
Species: Synthetic
Genetic Insert: Tq-Ca-FLITS
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34887382
Proper citation: RRID:Addgene_191454 Copy
Species: Synthetic
Genetic Insert: 10SUMO-6SIM
Vector Backbone Description: Vector Backbone:prs306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36108633
Proper citation: RRID:Addgene_190248 Copy
Species: Synthetic
Genetic Insert: dimer-SUMO
Vector Backbone Description: Vector Backbone:prs306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36108633
Proper citation: RRID:Addgene_190249 Copy
Species: Homo sapiens
Genetic Insert: PRPF4
Vector Backbone Description: Backbone Size:6580; Vector Backbone:pET28GST-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: insert+fusion tag if applicable MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSSMGSSHHHHHHSSGLVPRGSMQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE sequence of insert only (no fusion tag) MQELHKSLRSLNNFCSQIGDDRPISYCHFSPNSKMLATACWSGLCKLWSVPDCNLLHTLRGHNTNVGAIVFHPKSTVSLDPKDVNLASCAADGSVKLWSLDSDEPVADIEGHTVRVARVMWHPSGRFLGTTCYDRSWRLWDLEAQEEILHQEGHSMGVYDIAFHQDGSLAGTGGLDAFGRVWDLRTGRCIMFLEGHLKEIYGINFSPNGYHIATGSGDNTCKVWDLRQRRCVYTIPAHQNLVTGVKFEPIHGNFLLTGAYDNTAKIWTHPGWSPLKTLAGHEGKVMGLDISSDGQLIATCSYDRTFKLWMAE
Proper citation: RRID:Addgene_137650 Copy
Species: Homo sapiens
Genetic Insert: myocilin
Vector Backbone Description: Backbone Marker:unknown; Backbone Size:5542; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35196929
Proper citation: RRID:Addgene_192820 Copy
Species: Mus musculus
Genetic Insert: 3'UTR mMov10
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3560; Vector Backbone:psiCHECK-2; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35856394
Comments: Please visit https://doi.org/10.1101/2021.11.09.467897 for bioRxiv preprint.
Proper citation: RRID:Addgene_178910 Copy
Species: Homo sapiens
Genetic Insert: N-WASP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37226883
Proper citation: RRID:Addgene_191451 Copy
Species: Homo sapiens
Genetic Insert: HUWE1
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34314700
Proper citation: RRID:Addgene_187120 Copy
Species: Synthetic
Genetic Insert: G-Ca-FLITS
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41416770
Proper citation: RRID:Addgene_191465 Copy
Species: Synthetic
Genetic Insert: G-Ca-FLITS
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41416770
Proper citation: RRID:Addgene_191463 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:pCAMBIA1305.1; Vector Types:Plant Expression, binary vector for plant transformation; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34719678
Proper citation: RRID:Addgene_179835 Copy
Species: Synthetic
Genetic Insert: hexamer
Vector Backbone Description: Vector Backbone:prs304; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36108633
Proper citation: RRID:Addgene_190251 Copy
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