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Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.
Proper citation: RRID:Addgene_51481 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2400; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24820376
Comments: See www.wormbuilder.org for updated protocols.
Proper citation: RRID:Addgene_51483 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR4; Vector Types:Mammalian Expression, PiggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23979020
Comments: For more information including protocols and
updates, please go to http://www.crispr-on.org
Proper citation: RRID:Addgene_48234 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48863 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48859 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48858 Copy
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Golden Gate Compatible cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24376629
Comments: This plasmid is designed to take up inserts for future Golden Gate cloning using the BsaI enzyme. The 5' BsaI overhang can be exchanged using HindIII and BamHI. The 3' BsaI overhang can be exchanged using KpnI and EcoRI. BamHI and KpnI sites as well as the double XcmI sites can also be used as alternative insert cloning sites, instead of using BsaI.
Plasmid Features (listed as bp in full plasmid sequence):
BsaI site #1 = 2241-2251bp
BsaI site #2 = 3315-3325bp
Proper citation: RRID:Addgene_48857 Copy
Vector Backbone Description: Backbone Marker:Lorenz et al, 1996 (PubMed ID: 8917596); Backbone Size:5246; Vector Backbone:pTLN; Vector Types:Xenopus oocytes expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Iwan Zimmermann, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49032 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5855; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49030 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6578; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21410291
Comments: Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
Proper citation: RRID:Addgene_49029 Copy
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11157; Vector Backbone:pLEXiPuro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48957 Copy
Vector Backbone Description: Backbone Marker:Open Biosystems; Backbone Size:11164; Vector Backbone:pLEXiPuro; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Proper citation: RRID:Addgene_48956 Copy
Vector Backbone Description: Backbone Marker:Rajewsky Lab, PMID 18327259 ; Vector Backbone:pSiP1; Vector Types:Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24146852
Comments: The presence of firefly luciferase in the psiCHECK-2-derived vectors allows normalization of the Renilla luciferase expression that monitors the RNAi effect.
Proper citation: RRID:Addgene_48955 Copy
Vector Backbone Description: Backbone Marker:https://www.addgene.org/73638/; Backbone Size:5643; Vector Backbone:pDEST-ORF-V2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40419795
Proper citation: RRID:Addgene_217453 Copy
Vector Backbone Description: Backbone Marker:https://www.addgene.org/73636/; Backbone Size:7371; Vector Backbone:pDEST-V2-ORF; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40419795
Proper citation: RRID:Addgene_217454 Copy
Vector Backbone Description: Vector Backbone:pTcINDEX; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25424446
Comments: 5' Cloning Site: NruI (destroyed), 3' Cloning Site: NruI (destroyed).
Proper citation: RRID:Addgene_234713 Copy
Vector Backbone Description: Vector Backbone:pTcTurboID-GW; Vector Types:Trypanosomatid expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed).
Proper citation: RRID:Addgene_234714 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238493 Copy
Vector Backbone Description: Vector Backbone:Custom (R6K origin); Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:40048245
Proper citation: RRID:Addgene_238494 Copy
Vector Backbone Description: Vector Backbone:pUC57-pB-2xIns-Flk-iCre-WPRE-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229818 Copy
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