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Vector Backbone Description: Vector Backbone:pcDNA5-FRT-TOgw; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_223949 Copy
Genetic Insert: FRT10-STOP-FRT10
Vector Backbone Description: Vector Backbone:pBPGAL4DBD; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39677673
Comments: Please visit https://doi.org/10.1101/2024.12.02.626507 for bioRxiv preprint.
Proper citation: RRID:Addgene_232831 Copy
Vector Backbone Description: Vector Backbone:pCAGEN_DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229834 Copy
Vector Backbone Description: Vector Backbone:pB-pRAM-Flex-mClover3-KrasG12D-WPRE-pA-EFS-TetR; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229828 Copy
Vector Backbone Description: Vector Backbone:pCAGEN-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229815 Copy
Vector Backbone Description: Vector Backbone:pTol2-Hsp70-zCreI-mTagBFP2d-2x-ins; Vector Types:Tol2 destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229817 Copy
Vector Backbone Description: Vector Backbone:GLAST-pBase; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229822 Copy
Vector Backbone Description: Vector Backbone:pB_Tet-Off_Flex_DEST_EFS_mODC_tTA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_229821 Copy
Vector Backbone Description: Vector Backbone:Other; Vector Types:subcloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_231170 Copy
Vector Backbone Description: Vector Backbone:Other; Vector Types:subcloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_231172 Copy
Species: Synthetic
Genetic Insert: 3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-HF-BE3 from lacIq-Ptac promoter. Basal expression is sufficient for base editing in Coxiella burnetii. Note: During growth of E. coli harboring this plasmid, in some cells/colonies, the basal expression of 3xF-HF-BE3 can lead to spurious deamination of cytosines on the plasmid.
Unique XhoI, NotI and XbaI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid alias: pSAST356.
Proper citation: RRID:Addgene_221138 Copy
Species: Synthetic
Genetic Insert: 3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Comments: RSF1010-based plasmid. Expresses 3xF-BE4-PpAPOBEC1(H122A) from lacIq-Ptac promoter. IPTG induction is required for base editing in Coxiella burnetii. Unique XhoI, NotI and SapI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid encodes sacB, but sacB is not required for Coxiella burnetii base editing protocol. Plasmid alias: pSAST372.
Proper citation: RRID:Addgene_221139 Copy
Species: Other
Genetic Insert: ehxA
Vector Backbone Description: Vector Backbone:pBR325; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:3298451
Comments: Also reference Schmidt et al. I&I 63:1055-1061
Proper citation: RRID:Addgene_63754 Copy
Species: V. cholerae
Genetic Insert: bottom toxin fragment from a HindIII digest of V. cholerae 395 chromosome
Vector Backbone Description: Vector Backbone:pBR325; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Kaper et. al. (1984) Bio/Technology 2:345
Proper citation: RRID:Addgene_63689 Copy
Species: V. cholerae
Genetic Insert: V. cholera chromosomal DNA
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Kaper et. al. 1986. Advances in Research on Cholera and Related Diarrheas, 3 eds., S. Kuwahara, N.F. Pierce, 181-191. Copyright 1986 by KTK Scientific Publishers, Tokyo
Proper citation: RRID:Addgene_63691 Copy
Vector Backbone Description: Backbone Marker:Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 15; Backbone Size:7407; Vector Backbone:p414GPD; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25877921
Comments: Plasmid is a modified version of p414GPD from:
Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504
Proper citation: RRID:Addgene_63780 Copy
Species: Danio rerio
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Kwan et al., 2007; Vector Backbone:pDestTol2pA2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23444339
Proper citation: RRID:Addgene_64023 Copy
Species: Synthetic
Genetic Insert: rTTA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Mouse Targeting, Tet Inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24082130
Proper citation: RRID:Addgene_64103 Copy
Vector Backbone Description: Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25855067
Proper citation: RRID:Addgene_64241 Copy
Vector Backbone Description: Backbone Size:7083; Vector Backbone:pDestTol2LC; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25855067
Proper citation: RRID:Addgene_64242 Copy
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