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Species: Synthetic
Genetic Insert: CTG REPEATS
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Addgene cannot verify or guarantee the presence of all 700 CTG repeats in this array
Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63087 Copy
Species: Rattus norvegicus
Genetic Insert: Endolyn
Vector Backbone Description: Backbone Marker:Girotti and Banting, PMID 9013339 ; Vector Backbone:delta pMEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10620506
Proper citation: RRID:Addgene_62962 Copy
Species: Rattus norvegicus
Genetic Insert: lgp120
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The 10 amino acid linker was designed using the sequence as described below but with different cloning sites (EcoRI and BamHI):
Patterson GH and Lippincott-Schwartz J. (2002) A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873-1877.
Examples of use:
Jahreiss L, Menzies FM, Rubinsztein DC. (2008) The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes. Traffic 9: 574-587.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC. (2011) Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. J Cell Sci 124:469-482.
Ogunbayo OA, Zhu Y, Shen B, Agban E, Li J, Ma J, Zhu MX, Evans AM. (2015) Organelle-specific Subunit Interactions of the Vertebrate Two-pore Channel Family. J. Biol. Chem. 290: 1086-1095.
Proper citation: RRID:Addgene_62963 Copy
Species: Homo sapiens
Genetic Insert: CD63 molecule
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Examples of use:
Blagoveshchenskaya AD, Hannah MJ, Allen S, Cutler DF. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor. Mol Biol Cell 13:1582-1593.
Bampton ET, Goemans CG, Niranjan D, Mizushima N, Tolkovsky AM. (2005) The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes.
Autophagy 1: 23-36.
Harrison-Lavoie KJ, Michaux G, Hewlett L, Kaur J, Hannah MJ, Lui-Roberts WW, Norman KE, Cutler DF. (2006) P-selectin and CD63 use different mechanisms for delivery to Weibel-Palade bodies.
Traffic 7: 647-662.
Babich V, Meli A, Knipe L, Dempster JE, Skehel P, Hannah MJ, Carter T. (2008) Selective release of molecules from Weibel-Palade bodies during a lingering kiss. Blood 111: 5282-5290.
Babich V, Knipe L, Hewlett L, Meli A, Dempster J, Hannah MJ, Carter T. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
Proper citation: RRID:Addgene_62964 Copy
Species: Mus musculus
Genetic Insert: BTRC
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25632159
Proper citation: RRID:Addgene_62977 Copy
Species: Synthetic
Genetic Insert: FLIM-AKAR
Vector Backbone Description: Backbone Marker:Arpiar Saunders and Bernardo Sabatini; Backbone Size:5400; Vector Backbone:AAV-ChR2-mCherry; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24765076
Proper citation: RRID:Addgene_63058 Copy
Species: Mus musculus
Genetic Insert: PKIalpha
Vector Backbone Description: Backbone Marker:Yao Chen and Bernardo Sabatini; Backbone Size:6400; Vector Backbone:AAV-FLEX-tmeGFP-IRES-nls-mRuby2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24765076
Proper citation: RRID:Addgene_63059 Copy
Species: Homo sapiens
Genetic Insert: expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation).
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63089 Copy
Species: Synthetic
Genetic Insert: CCUG 1200x
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63088 Copy
Vector Backbone Description: Vector Backbone:pUC18T-mini-Tn7T-oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:15908923
Proper citation: RRID:Addgene_63121 Copy
Vector Backbone Description: Vector Backbone:pXR; Vector Types:Bacterial Expression, Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25736914
Proper citation: RRID:Addgene_63144 Copy
Species: Danio rerio
Genetic Insert: Cas9-T2A-GFP
Vector Backbone Description: Backbone Size:2765; Vector Backbone:pME-MCS (Tol2Kit #237); Vector Types:CRISPR, Tol2 Middle Entry vector for Gateway; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752963
Comments: Reference for zebrafish codon-optimized Cas9:
Jao, L.E., Wente, S.R., and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A 110, 13904-13909.
Proper citation: RRID:Addgene_63155 Copy
Vector Backbone Description: Backbone Size:8267; Vector Backbone:pDestTol2CG2 (Tol2Kit#395); Vector Types:CRISPR, Tol2 destination vector for Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:25752963
Comments: Reference for the Tol2Kit:
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy, M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P., and Chien, C.B. (2007). The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236, 3088-3099.
Proper citation: RRID:Addgene_63156 Copy
Genetic Insert: anti-secY-Pxyl/tetO-tetR
Vector Backbone Description: Backbone Marker:Arnaud et al. 2004. Applied and Environmental Microbiology, 70:6887-6891; Backbone Size:9666; Vector Backbone:pMAD; Vector Types:Suicide plasmid for Gram-positive bacteria; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26038185
Comments: Note: Addgene's quality control sequencing finds an H63R amino acid residue substitution in the TetR ORF. This residue change does not affect TetR activity, and the plasmid functions as described.
Proper citation: RRID:Addgene_63158 Copy
Species: Danio rerio
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2765; Vector Backbone:pME-MCS (Tol2Kit #237); Vector Types:CRISPR, Tol2 Middle Entry vector for Gateway; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25752963
Comments: Reference for zebrafish codon-optimized Cas9:
Jao, L.E., Wente, S.R., and Chen, W. (2013). Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system. Proc Natl Acad Sci U S A 110, 13904-13909.
Proper citation: RRID:Addgene_63154 Copy
Species: Drosophila melanogaster
Genetic Insert: "spaghetti monster GFP-HA"
Vector Backbone Description: Vector Backbone:pJFRC12-10XUAS-IVS-myr::GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25964354
Proper citation: RRID:Addgene_63166 Copy
Species: Drosophila melanogaster
Genetic Insert: "spaghetti monster GFP-FLAG"
Vector Backbone Description: Vector Backbone:pJFRC12-10XUAS-IVS-myr::GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25964354
Proper citation: RRID:Addgene_63169 Copy
Genetic Insert: DV4 prM-E
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29999491
Comments: Please visit https://www.biorxiv.org/content/early/2018/03/22/286955 for bioRxiv preprint.
Proper citation: RRID:Addgene_63164 Copy
Species: Drosophila melanogaster
Genetic Insert: "spaghetti monster GFP-OLLAS"
Vector Backbone Description: Vector Backbone:pJFRC12-10XUAS-IVS-myr::GFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25964354
Proper citation: RRID:Addgene_63170 Copy
Species: Mus musculus
Genetic Insert: MarsL274G
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26991063
Comments: Developed by Alborz Mahdavi.
Proper citation: RRID:Addgene_63177 Copy
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