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Species: Homo sapiens
Genetic Insert: truncated human Drosha mutant
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20554852
Proper citation: RRID:Addgene_62533 Copy
Species: Homo sapiens
Genetic Insert: human Drosha with serines 300 and 302 changed alanines
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20554852
Comments: Drosha contains H278R. Depositor states that this mutation should not affect function.
Proper citation: RRID:Addgene_62530 Copy
Species: Homo sapiens
Genetic Insert: CACNA1B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pcDNA6/V5-HisA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25296916
Comments: contains alternate exons (+e10a, +18a, Δ19a, +e31a, +e37b and +e46)
Note that the human CaV2.2 insert contains the native stop codon. The V5 and His tags present in the pcDNA6/V5-HisA backbone are NOT in frame with CaV2.2.
Proper citation: RRID:Addgene_62574 Copy
Species: Homo sapiens
Genetic Insert: hAlpha7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62629 Copy
Species: Homo sapiens
Genetic Insert: SPRED1
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25202123
Comments: There are a few mismatches outside of the mutated seed sequence; these mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_62580 Copy
Species: Homo sapiens
Genetic Insert: SPRED1
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:6470; Vector Backbone:pMIR-Report; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25202123
Comments: There are a few mismatches outside of the mutated seed sequence; these mismatches should not affect plasmid function.
Proper citation: RRID:Addgene_62581 Copy
Species: Homo sapiens
Genetic Insert: CHRFAM7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25056953
Proper citation: RRID:Addgene_62638 Copy
Species: Homo sapiens
Genetic Insert: Cerulean-P2A-PuroR
Vector Backbone Description: Backbone Size:3671; Vector Backbone:pUC57; Vector Types:Mammalian Expression, TALEN, homology-directed repair donor; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25765640
Comments: This plasmid contains the Cerulean fluorescent protein and loxP sites.
Proper citation: RRID:Addgene_62596 Copy
Species: Homo sapiens
Genetic Insert: MCAK
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62785 Copy
Species: Homo sapiens
Genetic Insert: TES
Vector Backbone Description: Vector Backbone:N1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62786 Copy
Species: Homo sapiens
Genetic Insert: Alpha5Integrin
Vector Backbone Description: Vector Backbone:PS-CFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62794 Copy
Species: Homo sapiens
Genetic Insert: Alpha5Integrin
Vector Backbone Description: Vector Backbone:PA-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62790 Copy
Species: Homo sapiens
Genetic Insert: Alpha5Integrin
Vector Backbone Description: Vector Backbone:PA-TagRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62791 Copy
Species: Homo sapiens
Genetic Insert: Tubulin
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62736 Copy
Species: Homo sapiens
Genetic Insert: Galectin
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62745 Copy
Species: Homo sapiens
Genetic Insert: CenpA
Vector Backbone Description: Vector Backbone:N1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62742 Copy
Species: Homo sapiens
Genetic Insert: ALK5
Vector Backbone Description: Vector Backbone:N1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62751 Copy
Species: Homo sapiens
Genetic Insert: BRAT1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25070371
Proper citation: RRID:Addgene_62802 Copy
Species: Homo sapiens
Genetic Insert: TPX2M2
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62765 Copy
Species: Homo sapiens
Genetic Insert: PML-IV
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pLPC; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21205865
Comments: The PML isoform in this plasmid is referred to as PML-IV according to the nomenclature established by Jensen, Shiels and Freemont Oncogene. 2001 Oct 29;20(49):7223-33. The GenBank nomenclature for PML-IV is PML isoform 6 (GenBank ID NM_002675.3).
Proper citation: RRID:Addgene_62804 Copy
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