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Species: Saccharomyces cerevisiae
Genetic Insert: ATG8
Vector Backbone Description: Vector Backbone:ClhN-MET15; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207034 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG8
Vector Backbone Description: Vector Backbone:ClhN-P1K-3VSV-Atg8-MET15; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207050 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG8
Vector Backbone Description: Vector Backbone:ClhnN-MET15; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207055 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ATG8
Vector Backbone Description: Vector Backbone:ClhN-P1K-3VSV-Atg8-MET15; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34088313
Proper citation: RRID:Addgene_207054 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cox4
Vector Backbone Description: Vector Backbone:ClhN-URA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35727034
Proper citation: RRID:Addgene_207062 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tgl3
Vector Backbone Description: Vector Backbone:ClhN-URA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35727034
Proper citation: RRID:Addgene_207066 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 4xV5-APEX2
Vector Backbone Description: Vector Backbone:ClhN-URA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35727034
Proper citation: RRID:Addgene_207063 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex3
Vector Backbone Description: Vector Backbone:ClhN-URA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35727034
Proper citation: RRID:Addgene_207069 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pex3
Vector Backbone Description: Vector Backbone:ClhN-URA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35727034
Proper citation: RRID:Addgene_207068 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BadBoy2 TP-DNAP1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_213785 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: BB-Tv TP-DNAP1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_213786 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: WT TP-DNAP1 (recoded)
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_213784 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SUC2
Vector Backbone Description: Vector Backbone:pYSD099; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38483353
Proper citation: RRID:Addgene_212925 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: site 2/Cas9/Eco1 RT
Vector Backbone Description: Vector Backbone:pZS.157; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39658047
Comments: Please visit https://doi.org/10.1101/2024.07.08.602561 for bioRxiv preprint.
Proper citation: RRID:Addgene_222226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScChd1 (aa118-1274)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6000; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173352
Proper citation: RRID:Addgene_221198 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ScChd1 (aa118-1274)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6000; Vector Backbone:pDEST17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35173352
Proper citation: RRID:Addgene_221207 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: redox(ro)GFP2-ORP1
Vector Backbone Description: Backbone Marker:Golden Gate Addgene number #48076; Backbone Size:9078; Vector Backbone:pICH86988; Vector Types:Plant Expression, Binary Agrobacterium Ti plasmid; Bacterial Resistance:Kanamycin
Comments: While versions of this fusion exists elswhere, ours was synthesised de novo. Please visit https://doi.org/10.1101/2024.01.18.576236 for bioRxiv preprint.
Proper citation: RRID:Addgene_191677 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PHO8∆60
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40458443
Proper citation: RRID:Addgene_240970 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP Recombinase
Vector Backbone Description: Backbone Marker:NA; Backbone Size:5209; Vector Backbone:pET28a(+)v2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease).
The total insert size displayed on this page (1872bp) includes all tags and linkers.
Proper citation: RRID:Addgene_246980 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: msGFP2-Vrg4
Vector Backbone Description: Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_247743 Copy
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