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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMXs-mGCN2-FLAG-IP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101794 | Gcn2 | Mus musculus | Ampicillin | PMID:27633668 | Backbone Marker:Dr. Toshio Kitamura; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 2 | ||
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pCDF1-mAtf4-HT-puro Resource Report Resource Website 1+ mentions |
RRID:Addgene_101795 | Activating transcription factor 4 | Mus musculus | Ampicillin | Backbone Marker:Systems Biosciences Inc; Backbone Size:6592; Vector Backbone:pCDF1-MCS2-EF1-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 1 | |||
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pGL3-mFgf21pro1-luc Resource Report Resource Website 1+ mentions |
RRID:Addgene_101797 | Fibroblast growth factor 21 promoter | Mus musculus | Ampicillin | PMID:26487695 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 3 | ||
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px330 VRER Resource Report Resource Website 1+ mentions |
RRID:Addgene_101729 | SpCas9 VRER | S pyogenes | Ampicillin | PMID:29804665 | Cas9 VRER sequence cloned from Addgene Plasmid #65773 cloned into px330 Addgene plasmid #42230 | Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | VRER (D1135V, G1218R, R1335E, and T1337R mutations in Cas9) | 2026-08-15 01:00:21 | 2 |
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pCSN061 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101725 | S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. | Ampicillin | PMID:28886218 | Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 2 | |||
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p458 VRER Resource Report Resource Website 1+ mentions |
RRID:Addgene_101728 | SpCas9 VRER | S pyogenes | Ampicillin | PMID:29804665 | Cas9 VRER sequence cloned from Addgene Plasmid #65773 cloned into px458 Addgene plasmid #48138 | Vector Backbone:px458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | VRER (D1135V, G1218R, R1335E, and T1337R mutations in Cas9) | 2026-08-15 01:00:21 | 2 |
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pCRRNA-G20-R18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101817 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:22 | 1 | ||
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pCRRNA-G0-R20 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101818 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:21 | 1 | ||
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pCRRNA-G11-R18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101815 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:21 | 1 | ||
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pCRRNA-G14-R18 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101816 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:21 | 1 | ||
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pMH-SFB-DYRK1A Resource Report Resource Website 1+ mentions |
RRID:Addgene_101770 | DYRK1A | Homo sapiens | Ampicillin | PMID:38966995 | Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | siRNA-resistant allele | 2026-08-15 01:00:21 | 1 | |
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pMH-SFB-DYRK1B Resource Report Resource Website 1+ mentions |
RRID:Addgene_101771 | DYRK1B / MIRK | Homo sapiens | Ampicillin | PMID:32611815 | Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | siRNA-resistant allele | 2026-08-15 01:00:21 | 1 | |
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pCRRNA-G14-R11 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101811 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:22 | 1 | ||
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H14-MBP-SUMO-Cys-Linker-BAF_G47E Resource Report Resource Website 1+ mentions |
RRID:Addgene_101779 | BAF (BANF1) | Homo sapiens | Kanamycin | PMID:28841419 | Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | G47E mutation | 2026-08-15 01:00:21 | 1 | |
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EGFP-P2A_BAF_G47E Resource Report Resource Website 1+ mentions |
RRID:Addgene_101774 | BAF (BANF1) | Homo sapiens | Ampicillin | PMID:28841419 | Backbone Marker:Feng Zhang; Backbone Size:10370; Vector Backbone:Addgene plasmid # 52962; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | G47E mutation | 2026-08-15 01:00:21 | 1 | |
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YORWΔ22α1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101708 | Kanamycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2α1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:20 | 1 | ||||
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YPRCΔ15Ω2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101709 | Spectinomycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:00:21 | 1 | ||||
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YORWΔ22Ω2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101705 | Spectinomycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:00:20 | 1 | ||||
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YORWΔ22Ω1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101706 | Spectinomycin | PMID:29132331 | Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin | 2026-08-15 01:00:21 | 1 | ||||
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AICSDP-22:CETN2-mTagRFP-T Resource Report Resource Website 1+ mentions |
RRID:Addgene_101781 | CETN2 Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2026-08-15 01:00:21 | 1 |
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