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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pTXB1-Tn5 Resource Report Resource Website 10+ mentions |
RRID:Addgene_60240 | tnpA from pBam1 | E. coli | Ampicillin | PMID:25079858 | Backbone Marker:New England Biolabs; Backbone Size:6706; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | E54K, L372P | 2026-08-15 01:17:30 | 41 | |
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pQE-80L MBP-SspB Nano Resource Report Resource Website 1+ mentions |
RRID:Addgene_60409 | SspB nano | E. Coli | Ampicillin | PMID:25535392 | The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS | Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Y11K and A15E | 2026-08-15 01:17:32 | 9 |
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pQE-80L MBP-SspB Micro Resource Report Resource Website 1+ mentions |
RRID:Addgene_60410 | SspB micro | E. Coli | Ampicillin | PMID:25535392 | The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS | Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | R73Q, Y11K and A15E | 2026-08-15 01:17:32 | 3 |
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pZS1[GalS-T] Resource Report Resource Website |
RRID:Addgene_60753 | GalS-T | E. Coli | Ampicillin | PMID:25035932 | Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Chimeric LacI/GalR repressor with the TAN DBD and the FruR LBD. E191K | 2026-08-15 01:17:35 | 0 | |
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pZS1[FruR-T] Resource Report Resource Website |
RRID:Addgene_60752 | FruR-T | E. Coli | Ampicillin | PMID:25035932 | Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Chimeric LacI/GalR repressor with the TAN DBD and the FruR LBD. | 2026-08-15 01:17:36 | 0 | |
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pZS1[RbsR-T] Resource Report Resource Website |
RRID:Addgene_60751 | RbsR-T | E. Coli | Ampicillin | PMID:25035932 | Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Chimeric LacI/GalR repressor with the TAN DBD and the RbsR LBD. | 2026-08-15 01:17:35 | 0 | |
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pZS1[TreR-T] Resource Report Resource Website 1+ mentions |
RRID:Addgene_60754 | TreR-T | E. Coli | Ampicillin | PMID:25035932 | Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | Chimeric LacI/GalR repressor with the TAN DBD and the TreR LBD. | 2026-08-15 01:17:35 | 1 | |
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pPL5628_TEF1*-Cre_MET15 Resource Report Resource Website |
RRID:Addgene_60933 | Cre | E. coli | Ampicillin | PMID:25688547 | This plasmid contains a Y191CL mutation in the MET15 gene that does not impact selection or plasmid function. | Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 0 | |
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pPL5608_TEF1*-Cre_TRP1 Resource Report Resource Website |
RRID:Addgene_60931 | Cre | E. coli | Ampicillin | PMID:25688547 | Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:17:37 | 0 | ||
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pJB042 (FtsZ-mEos2) Resource Report Resource Website 1+ mentions |
RRID:Addgene_49764 | FtsZ-mEos2 | E. coli | Chloramphenicol | PMID:23859153 | Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:16:03 | 1 | ||
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pHL 1032 Resource Report Resource Website |
RRID:Addgene_52997 | RhyB sRNA | E. coli | Ampicillin | PMID:21189298 | Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:26 | 0 | ||
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pHL 1033 Resource Report Resource Website |
RRID:Addgene_52998 | RhyB sRNA | E. coli | Ampicillin | PMID:21189298 | Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:27 | 0 | ||
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pHL 1394 Resource Report Resource Website |
RRID:Addgene_53009 | RhyB sRNA | E. coli | Ampicillin | PMID:21189298 | Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:31 | 0 | ||
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pBRα-σ70 D581G Resource Report Resource Website |
RRID:Addgene_53732 | RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613) | E. coli | Ampicillin | PMID:9121589 | This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | The sigma 70 moiety carries the D581G substitution | 2026-08-15 01:16:33 | 0 |
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pTH Resource Report Resource Website |
RRID:Addgene_56659 | KanR | E. coli | Kanamycin | PMID:25302562 | Note: There are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. They should not affect the function of the plasmid. | Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Synthetic Biology, Diatom Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:17:00 | 0 | |
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pTXB1-CRP/CAP Resource Report Resource Website |
RRID:Addgene_51563 | CRP/CAP | E. coli | Ampicillin | Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:15 | 0 | |||
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pBAD24-rnc-sfGFP Resource Report Resource Website |
RRID:Addgene_51560 | rnc | E. coli | Ampicillin | PMID:23893733 | Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains additonal aa at the end of the sfGFP in the N and C terminus (in frame with the polylinker) | 2026-08-15 01:16:19 | 0 | |
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C321 Resource Report Resource Website 1+ mentions |
RRID:Addgene_48999 | Recoded E. coli genome with all instances of the UAG codon removed, but wild type UAG termination function is not changed | E. coli | Ampicillin | PMID:24136966 | E. coli MG1655 delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta mutS::zeoR; all 321 UAG codons changed to UAA This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin. Sequence is similar to C321.DA (NCBI accession # NCBI CP006698), except that RF1 is not deleted. | Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | See genbank file of C321.DA | 2026-08-15 01:15:55 | 3 |
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Ec ssb-pET(3d) Resource Report Resource Website |
RRID:Addgene_237240 | ssb (SSB) | E. coli | Ampicillin | Vector Backbone:pET-3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:32:56 | 0 | |||
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pET30a-SSDH Resource Report Resource Website 1+ mentions |
RRID:Addgene_239573 | Succinate-semialdehyde dehydrogenase | E. coli | Kanamycin | PMID:40656557 | Backbone Size:5408; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:33:11 | 1 |
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