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Species: E. coli
Genetic Insert: tnpA from pBam1
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6706; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25079858
Proper citation: RRID:Addgene_60240 Copy
Species: E. Coli
Genetic Insert: SspB nano
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60409 Copy
Species: E. Coli
Genetic Insert: SspB micro
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60410 Copy
Species: E. Coli
Genetic Insert: GalS-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60753 Copy
Species: E. Coli
Genetic Insert: FruR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60752 Copy
Species: E. Coli
Genetic Insert: RbsR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60751 Copy
Species: E. Coli
Genetic Insert: TreR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60754 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Comments: This plasmid contains a Y191CL mutation in the MET15 gene that does not impact selection or plasmid function.
Proper citation: RRID:Addgene_60933 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Proper citation: RRID:Addgene_60931 Copy
Species: E. coli
Genetic Insert: FtsZ-mEos2
Vector Backbone Description: Backbone Marker:NBRP-E.coli at NIG; Backbone Size:4500; Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23859153
Proper citation: RRID:Addgene_49764 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_52997 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_52998 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53009 Copy
Species: E. coli
Genetic Insert: RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53732 Copy
Species: E. coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Synthetic Biology, Diatom Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25302562
Comments: Note: There are some small discrepancies between Addgene's quality control sequence and the assembled sequence from the depositor. They should not affect the function of the plasmid.
Proper citation: RRID:Addgene_56659 Copy
Species: E. coli
Genetic Insert: CRP/CAP
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51563 Copy
Species: E. coli
Genetic Insert: rnc
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Proper citation: RRID:Addgene_51560 Copy
Species: E. coli
Genetic Insert: Recoded E. coli genome with all instances of the UAG codon removed, but wild type UAG termination function is not changed
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24136966
Comments: E. coli MG1655 delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta mutS::zeoR; all 321 UAG codons changed to UAA
This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.
Sequence is similar to C321.DA (NCBI accession # NCBI CP006698), except that RF1 is not deleted.
Proper citation: RRID:Addgene_48999 Copy
Species: E. coli
Genetic Insert: ssb (SSB)
Vector Backbone Description: Vector Backbone:pET-3d; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_237240 Copy
Species: E. coli
Genetic Insert: Succinate-semialdehyde dehydrogenase
Vector Backbone Description: Backbone Size:5408; Vector Backbone:pET30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40656557
Proper citation: RRID:Addgene_239573 Copy
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