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Species: Homo sapiens
Genetic Insert: LAMP1 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human LAMP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EFGSTGSTGSTGADPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101782 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101821 Copy
Species: Synthetic
Genetic Insert: 3xERSE-I
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.29; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32577017
Proper citation: RRID:Addgene_101789 Copy
Species: Homo sapiens
Genetic Insert: SLC25A17 Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SLC25A17 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: RDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101784 Copy
Species: Homo sapiens
Genetic Insert: TUBA1B Homology Arms with mTagRFPT-linker
Vector Backbone Description: Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101785 Copy
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28886218
Proper citation: RRID:Addgene_101750 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29519933
Proper citation: RRID:Addgene_101804 Copy
Vector Backbone Description: Vector Backbone:pMH-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_101765 Copy
Vector Backbone Description: Vector Backbone:pMH-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28325877
Proper citation: RRID:Addgene_101768 Copy
Species: Homo sapiens
Genetic Insert: SRY-box 2 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101761 Copy
Species: Homo sapiens
Genetic Insert: transforming growth factor beta 1 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101762 Copy
Species: Homo sapiens
Genetic Insert: SMAD2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29593326
Proper citation: RRID:Addgene_101763 Copy
Vector Backbone Description: Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4378; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin
Comments: Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
Proper citation: RRID:Addgene_101854 Copy
Vector Backbone Description: Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4835; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin
Comments: Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html
Proper citation: RRID:Addgene_101855 Copy
Species: Mus musculus
Genetic Insert: Ascl1, Brn2
Vector Backbone Description: Backbone Size:5845; Vector Backbone:pUC19; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28646119
Proper citation: RRID:Addgene_101852 Copy
Species: Mus musculus
Genetic Insert: Evi-1
Vector Backbone Description: Vector Backbone:pLV-EF1a-PGK-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101866 Copy
Species: Mus musculus
Genetic Insert: Evi-1
Vector Backbone Description: Vector Backbone:pLV-EF1a-PGK-BFPT2aPuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101867 Copy
Species: Mus musculus
Genetic Insert: Mds1-Evi-1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101860 Copy
Species: NA
Genetic Insert: Kanamycin casette (in place of synpcc7942_2333-2335 operon)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101839 Copy
Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431A T432A with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101837 Copy
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