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Species: Rattus norvegicus
Genetic Insert: OCT-HA-eGFP
Vector Backbone Description: Vector Backbone:pCMV(delta3); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27057421
Comments: Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the matrix using the leader sequence from ornithine carbamylase transferase (OCT): Horwich, et al (1985) EMBO J (PMCID: 554314); and (3) Harder, et al (2004) Current Biology 14:340-348
Proper citation: RRID:Addgene_67482 Copy
Species: Rattus norvegicus
Genetic Insert: mRuby2-P2A-GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284193
Comments: The discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequence.
mRuby2:
Article: Improving FRET dynamic range with bright green and red fluorescent proteins. Lam et al (Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171. PubMed)
Addgene Plasmid 40260
GCaMP6f:
Article: Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen et al (Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. PubMed)
Addgene Plasmid 40755
Proper citation: RRID:Addgene_68719 Copy
Species: Rattus norvegicus
Genetic Insert: G-alpha-s-EE-mCerulean
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15297467
Comments: Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675
Reference for mCerulean: Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
Proper citation: RRID:Addgene_66084 Copy
Species: Rattus norvegicus
Genetic Insert: G-alpha-s-EE-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15297467
Comments: Reference for alpha-s-EE: Wilson, P. T., and Bourne, H. R. (1995) Fatty acylation of αz. Effects of palmitoylation and myristoylation on αz signaling. J. Biol. Chem. 270, 9667-9675
Proper citation: RRID:Addgene_66083 Copy
Species: Rattus norvegicus
Genetic Insert: Opto Mu opioid receptor
Vector Backbone Description: Backbone Size:6330; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25937173
Proper citation: RRID:Addgene_66847 Copy
Species: Rattus norvegicus
Genetic Insert: ErbB2
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16352662
Proper citation: RRID:Addgene_66945 Copy
Species: Rattus norvegicus
Genetic Insert: ErbB2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1D/V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17626164
Comments: A24T is a genetic variation and has no effect on function.
Proper citation: RRID:Addgene_66946 Copy
Species: Rattus norvegicus
Genetic Insert: NCX3.1
Vector Backbone Description: Vector Backbone:pBS?; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8798769
Proper citation: RRID:Addgene_67147 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28423323
Proper citation: RRID:Addgene_114113 Copy
Species: Rattus norvegicus
Genetic Insert: Erythropoietin Receptor
Vector Backbone Description: Vector Backbone:pCWXPGR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114289 Copy
Species: Rattus norvegicus
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:p2K7; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114311 Copy
Species: Rattus norvegicus
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114310 Copy
Species: Rattus norvegicus
Genetic Insert: IRS-3
Vector Backbone Description: Backbone Marker:pBABE-bleo/zeo is available at Addgene; Backbone Size:4888; Vector Backbone:pBABE bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11113178
Comments: Rat IRS-3 was generated by PCR from the rat genomic DNA sequence. The intron was removed by PCR overlap extension using the following two pairs of primers: 5'-CGTGGATCCGCGATGAAGCCTGCAGGTACG-3' (sense) plus 5'-CTTGGGGGCTGAAACCCATGTTTGCTGGGCA-3' (antisense) and 5'-TGCCCAGCAAACATGGGTTTCAGCCCCCAAG-3' (sense) plus 5'-GCTGTCGACGTTCTAGAACTTGATGCTG-3' (antisense). BamHI and SalI sites were introduced at the 5' and 3' ends, respectively, of the coding sequence of IRS-3 by PCR. The subsequently generated coding sequence of rat IRS-3 was ligated into BamHI and SalI sites in pBABE-bleo.
Proper citation: RRID:Addgene_11451 Copy
Species: Rattus norvegicus
Genetic Insert: shRNA targeting Plxnb2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29981480
Proper citation: RRID:Addgene_115175 Copy
Species: Rattus norvegicus
Genetic Insert: rat PAM3
Vector Backbone Description: Backbone Marker:Cornelia Gorman; Backbone Size:5000; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8294504
Comments: Please note: Plasmid contains a Valine 221 deletion in rPAM3 compared to the NCBI reference sequence AAC05608.1. This deletion is not known to affect protein activity.
Proper citation: RRID:Addgene_145803 Copy
Species: Rattus norvegicus
Genetic Insert: beta-arrestin 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Proper citation: RRID:Addgene_14692 Copy
Species: Rattus norvegicus
Genetic Insert: TGF beta type I receptor
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8662796
Comments: See Author's map for more information.
A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.
Proper citation: RRID:Addgene_14833 Copy
Species: Rattus norvegicus
Genetic Insert: YFP-PKCbetaII-YFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: YFP-PKCbetaII-YFP was made by PCR of mYFP from HindII to KpnI, rat PKCbetaII from KpnI to XhoI, and YFP from XhoI to XbaI.
Proper citation: RRID:Addgene_14866 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8626492
Comments: cDNA clone isolated from a rat islet cDNA library constructed in pcDNA-1. The full length insert was excised by KpnI/XbaI and subcloned into pcDNA3.
Proper citation: RRID:Addgene_14946 Copy
Species: Rattus norvegicus
Genetic Insert: Rat insulin promoter (RIP)
Vector Backbone Description: Backbone Size:6700; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15129273
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
The RIP–CreER construct was generated by fusing a 0.66-kilobase SmaI–HindIII fragment of the RIP2 promoter to a minimal hsp68 promoter, and placing the chimaeric promoter upstream of CreER(TM).
See Author's Map for more information.
Proper citation: RRID:Addgene_15029 Copy
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