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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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AICSDP-19:LAMP1-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101782 | LAMP1 Homology Arms with linker-mEGFP | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human LAMP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EFGSTGSTGSTGADPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 3 | ||
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pCRRNA-G14-R20 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101821 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:21 | 1 | ||
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pGL4-ERSE1-luc2P-Hygro Resource Report Resource Website 1+ mentions |
RRID:Addgene_101789 | 3xERSE-I | Synthetic | Ampicillin | PMID:32577017 | Backbone Marker:Promega; Vector Backbone:pGL4.29; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 4 | ||
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AICSDP-27:SLC25A17-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101784 | SLC25A17 Homology Arms with linker-mEGFP | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SLC25A17 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: RDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2026-08-15 01:00:21 | 1 | |
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AICSDP-28:TUBA1B-mTagRFP-T Resource Report Resource Website 1+ mentions |
RRID:Addgene_101785 | TUBA1B Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | 2026-08-15 01:00:21 | 1 | |
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pRN1120 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101750 | Ampicillin | PMID:28886218 | Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 2 | ||||
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pCRRNA-G10-R0 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101804 | crRNAs targeting GFP and mCherry | Synthetic | Kanamycin | PMID:29519933 | Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:21 | 1 | ||
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pMH-MYC Resource Report Resource Website 1+ mentions |
RRID:Addgene_101765 | Chloramphenicol and Ampicillin | PMID:28325877 | Vector Backbone:pMH-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:00:21 | 5 | ||||
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pMH-MBP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101768 | Chloramphenicol and Ampicillin | PMID:28325877 | Vector Backbone:pMH-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin | 2026-08-15 01:00:21 | 1 | ||||
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pGL3-Sox2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101761 | SRY-box 2 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 7 | ||
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pGL3-TGFb1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101762 | transforming growth factor beta 1 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:21 | 6 | ||
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CS2 Flag-Smad2 (3A) Resource Report Resource Website 1+ mentions |
RRID:Addgene_101763 | SMAD2 | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Three Ser to Ala mutations in the phosphorylation sites (S464A, S465A, S467A) | 2026-08-15 01:00:21 | 1 | |
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pCB1532B-RFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101854 | Ampicillin | Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html | Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4378; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | ||||
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pCB1532H-RFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101855 | Ampicillin | Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html | Backbone Marker:Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53 http://www.fgsc.net/fgn44/sweig.html; Backbone Size:4835; Vector Backbone:pCB1532; Vector Types:Fungal expression plasmid, tested in Magnaporthe; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | ||||
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U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE Resource Report Resource Website 1+ mentions |
RRID:Addgene_101852 | Ascl1, Brn2 | Mus musculus | Ampicillin | PMID:28646119 | Backbone Size:5845; Vector Backbone:pUC19; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 2 | ||
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pKAM-E-Flag-GFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101866 | Evi-1 | Mus musculus | Ampicillin | Vector Backbone:pLV-EF1a-PGK-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | |||
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pKAM-E-Flag-BFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101867 | Evi-1 | Mus musculus | Ampicillin | Vector Backbone:pLV-EF1a-PGK-BFPT2aPuro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | |||
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pcDNA3.1-ME-Flag Resource Report Resource Website 1+ mentions |
RRID:Addgene_101860 | Mds1-Evi-1 | Mus musculus | Ampicillin | Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:00:22 | 1 | |||
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zwf-fbp-opcA- Resource Report Resource Website 1+ mentions |
RRID:Addgene_101839 | Kanamycin casette (in place of synpcc7942_2333-2335 operon) | NA | Kanamycin | PMID:28430105 | Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin | 2026-08-15 01:00:22 | 1 | ||
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P0050::kaiBC S431A T432A NS1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101837 | kaiBC S431A T432A with p0050 promoter | S. elongatus PCC7942 | Spectinomycin and Streptomycin | PMID:28430105 | Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin | S431A T432A | 2026-08-15 01:00:22 | 1 |
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