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Species: Mus musculus
Genetic Insert: Galpha-i2
Vector Backbone Description: Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11802169
Comments: NcoI digestion and SP6 RNA polymerase for antisense probe
Proper citation: RRID:Addgene_15929 Copy
Species: Mus musculus
Genetic Insert: gRNA library targeting mouse genes
Vector Backbone Description: Backbone Marker:Jason Moffat; Vector Backbone:pLCKO2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32968282
Proper citation: RRID:Addgene_159393 Copy
Species: Mus musculus
Genetic Insert: pr.tac-BDNF (Bdnf, Mouse)
Vector Backbone Description: Backbone Marker:iGEM parts registry; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32948587
Proper citation: RRID:Addgene_159394 Copy
Species: Mus musculus
Genetic Insert: pr.tet-BDNF (Bdnf, Mouse)
Vector Backbone Description: Backbone Marker:iGEM parts registry; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32948587
Proper citation: RRID:Addgene_159410 Copy
Species: Mus musculus
Genetic Insert: pr.J23101-BDNF (Bdnf, Mouse)
Vector Backbone Description: Backbone Marker:iGEM parts registry; Vector Backbone:pSB1A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32948587
Proper citation: RRID:Addgene_159411 Copy
Species: Mus musculus
Genetic Insert: gRNA library targeting mouse core intrinsic immune evasion genes
Vector Backbone Description: Backbone Marker:Jason Moffat; Vector Backbone:pLCKO2; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32968282
Proper citation: RRID:Addgene_159391 Copy
Species: Mus musculus
Genetic Insert: M12
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBSIISK-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17662950
Comments: to make RNA probe, cut with NotI. Then do in vitro transcription with T7RNA polymerase.
Proper citation: RRID:Addgene_15955 Copy
Species: Mus musculus
Genetic Insert: DHS-4D
Vector Backbone Description: Vector Backbone:pMin-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34143204
Proper citation: RRID:Addgene_159653 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter PH2 domain
Vector Backbone Description: Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12488434
Proper citation: RRID:Addgene_12163 Copy
Species: Mus musculus
Genetic Insert: Foxo1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16154098
Comments: Note that a BLAST search with Addgene's quality control sequence shows a K219R mutation. The Accili lab has explained that this is a common variant and they do not consider it a mutation at all. They have used the plasmid routinely without issue. The plasmid also contains an L619P polymorphism.
Proper citation: RRID:Addgene_12148 Copy
Species: Mus musculus
Genetic Insert: PD-L1
Vector Backbone Description: Vector Backbone:pGIPz; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27572267
Proper citation: RRID:Addgene_121488 Copy
Species: Mus musculus
Genetic Insert: Dnmt1 shRNA
Vector Backbone Description: Backbone Size:7560; Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15240889
Comments: Cre TURNS ON Dmnt1 shRNA expression. The shRNA is directed against the mouse Dnmt1 sequence 5'-GAGTGTGTGAGGGAGAAA-3'.
Proper citation: RRID:Addgene_12165 Copy
Species: Mus musculus
Genetic Insert: MEKK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9528810
Comments: Please note that Addgene's sequencing results identified a V1467L mutation in the MEKK1 sequence when compared to GenBank ID AF117340.1. The depositing laboratory states that this plasmid functions exactly as intended and the mutation may represent a valid polymorphism.
Proper citation: RRID:Addgene_12181 Copy
Species: Mus musculus
Genetic Insert: MEKK2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9305638
Proper citation: RRID:Addgene_12183 Copy
Species: Mus musculus
Genetic Insert: MEKK2
Vector Backbone Description: Backbone Size:4600; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8621389
Proper citation: RRID:Addgene_12182 Copy
Species: Mus musculus
Genetic Insert: MEKK3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:14634666
Proper citation: RRID:Addgene_12189 Copy
Species: Mus musculus
Genetic Insert: IL-4 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:na; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10970869
Comments: Murine IL-4 promotor from -804 to -3 relative to the transcription start site.
Proper citation: RRID:Addgene_12195 Copy
Species: Mus musculus
Genetic Insert: IL-2 promoter
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:0; Vector Backbone:pT81Luc; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10970869
Comments: Murine IL-2 promotor from -585 to -34 relative to the transcription start site.
Proper citation: RRID:Addgene_12194 Copy
Species: Mus musculus
Genetic Insert: GBS(long)-GFP-insulator
Vector Backbone Description: Vector Backbone:pJC4-5; Vector Types:mouse transgenesis; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22265416
Proper citation: RRID:Addgene_121975 Copy
Species: Mus musculus
Genetic Insert: MD(ND2bHLH)WCS
Vector Backbone Description: Backbone Size:6667; Vector Backbone:pRRLSIN.cPPT.PGK.WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25801030
Proper citation: RRID:Addgene_122056 Copy
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