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Species: Synthetic
Genetic Insert: Synthetic CRISPR array containing four identical T. thermophilus spacers
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3920; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31278272
Proper citation: RRID:Addgene_127764 Copy
Genetic Insert: Empty backbone
Vector Backbone Description: Vector Backbone:pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: pSpCas9(BB)-2A-Puro (PX459) V2.0 was digested with EcoRI to remove the T2A-Puro cassette, and a P2A-Neomycin cassette was amplified by PCR, digested with EcoRI, and ligated.
Proper citation: RRID:Addgene_127762 Copy
Species: Synthetic
Genetic Insert: Convergent LacI-TetR Toggle
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682205 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127761 Copy
Genetic Insert: mCherry-mCherry
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30006597
Proper citation: RRID:Addgene_127803 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf90
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12778 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf87
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12775 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf88
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12776 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf85
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12773 Copy
Species: Synthetic
Genetic Insert: AsCpf1
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31316073
Proper citation: RRID:Addgene_127750 Copy
Species: Synthetic
Genetic Insert: mSpinach,RFP Divergent Orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682222 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127756 Copy
Species: Synthetic
Genetic Insert: mSpinach, MG RNA aptamer in divergent orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682223 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127759 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf91
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12779 Copy
Species: Synthetic
Genetic Insert: mSpinach, MG RNA aptamer in convergent orientation
Vector Backbone Description: Vector Backbone:pBEST; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28734826
Comments: Cloning method: Golden Gate. Genbank Accession No. KX682223 . Can also use growth strains: DH5Z1 or MG1655Z1 . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_127758 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127746 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127745 Copy
Species: Synthetic
Genetic Insert: acetylating acetaldehyde dehydrogenase
Vector Backbone Description: Vector Backbone:pDSM-f3; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACDH transcription unit with pathway position 7 homology arms, compatible with 5' position 6 and 3' ChrXV (3int) homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127744 Copy
Species: Synthetic
Genetic Insert: nourseothricin N-acetyltransferase
Vector Backbone Description: Vector Backbone:pDSM-ab; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: NatMX transcription unit with pathway position 2 homology arms, compatible with 5' position 1 and 3' position 3 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127747 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: aconitase
Vector Backbone Description: Vector Backbone:pDSM-cd; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: ACO transcription unit with pathway position 4 homology arms, compatible with 5' position 3 and 3' position 5 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127732 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: pyruvate carboxylase
Vector Backbone Description: Vector Backbone:pDSM-de; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29753070
Comments: PYC transcription unit with pathway position 5 homology arms, compatible with 5' position 4 and 3' position 6 homology arm. Sequencing primers are also the primers for amplifying DNA for integration into the S. cerevisiae genome.
Proper citation: RRID:Addgene_127736 Copy
Genetic Insert: BE4max-NG without deaminase
Vector Backbone Description: Vector Backbone:pBT195; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31332326
Comments: Benchling map: https://benchling.com/s/seq-x06ZpArOzposNFwSvQ95
Proper citation: RRID:Addgene_127844 Copy
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