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Species: Homo sapiens
Genetic Insert: dCas9-HA-BFP-KRAB-NLS
Vector Backbone Description: Backbone Size:9651; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Comments: Contains HA tag and TagBFP fusion. The plasmid is derived from Addgene Plasmid #46911. The promoter was changed to human EF1a and the NLS was moved to the C-terminus of the fusion protein.
Proper citation: RRID:Addgene_102244 Copy
Species: S. elongatus PCC7942
Genetic Insert: RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101831 Copy
Species: Homo sapiens
Genetic Insert: LAMP-mCherry-CRY2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25963241
Proper citation: RRID:Addgene_102249 Copy
Species: Homo sapiens
Genetic Insert: CHMP2B
Vector Backbone Description: Backbone Size:6098; Vector Backbone:pTO_LAP_N-DEST; Vector Types:Mammalian Expression, Bacterial Expression, Flp-FRT recombination, Tet-inducible expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28604678
Comments: Part of the plasmid was derived from pmCherry_N_dest from the Helenius Lab (ETH Zuerich)
Proper citation: RRID:Addgene_101848 Copy
Species: Rattus norvegicus
Genetic Insert: Kif5A-GFP-CIB1
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25963241
Proper citation: RRID:Addgene_102252 Copy
Species: NA
Genetic Insert: Kanamycin casette (in place of glgC gene)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101842 Copy
Species: E. coli
Genetic Insert: Ptrc promoter driving GalP transporter expression
Vector Backbone Description: Backbone Size:5300; Vector Backbone:pAM1303; Vector Types:Cloning vector; Bacterial Resistance:Nourseothricin (clonNat)
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101843 Copy
Species: NA
Genetic Insert: Kan casette followed by ptrc promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101844 Copy
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:29164072
Comments: Genotype = ΔompA
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]
Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene
Proper citation: RRID:Addgene_102256 Copy
Species: Homo sapiens
Genetic Insert: METTL14
Vector Backbone Description: Vector Backbone:pFBOH-LIC notag; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: none. SGC Clone Sample ID: METTL14:JMC097-G06:C232534. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
Proper citation: RRID:Addgene_101891 Copy
Species: Homo sapiens
Genetic Insert: METTL3
Vector Backbone Description: Backbone Marker:Cheryl Arrowsmith (Addgene plasmid # 62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL3:JMC094-C09:C231635. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
Proper citation: RRID:Addgene_101892 Copy
Vector Backbone Description: Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28474669
Proper citation: RRID:Addgene_101930 Copy
Vector Backbone Description: Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28474669
Proper citation: RRID:Addgene_101931 Copy
Vector Backbone Description: Backbone Marker:Bassik lab (Addgene Plasmid #89359); Vector Backbone:pMCB320; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28474669
Proper citation: RRID:Addgene_101933 Copy
Species: Synthetic
Genetic Insert: Lifeact
Vector Backbone Description: Backbone Size:8690; Vector Backbone:pMuLE Dest PuroR; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_101893 Copy
Species: NA
Genetic Insert: kanamycin casette and p0050 promoter
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101828 Copy
Species: Mus musculus
Genetic Insert: U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9
Vector Backbone Description: Backbone Marker:Gene Transfer Vector Core University of Iowa; Backbone Size:5679; Vector Backbone:pacAd5; Vector Types:Adenoviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28695888
Proper citation: RRID:Addgene_101824 Copy
Species: Chinese hamster
Genetic Insert: N-ethylmaleimide sensitive factor
Vector Backbone Description: Vector Backbone:pPROEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25581794
Proper citation: RRID:Addgene_102239 Copy
Species: Rhodothermus marinus DSM4252
Genetic Insert: β-glucosidase
Vector Backbone Description: Backbone Size:5711; Vector Backbone:pET16b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29516725
Proper citation: RRID:Addgene_101912 Copy
Species: Homo sapiens
Genetic Insert: SNAP47
Vector Backbone Description: Backbone Size:1341; Vector Backbone:pEGFP C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26359495
Proper citation: RRID:Addgene_101915 Copy
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