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Species: Homo sapiens
Genetic Insert: DSN1, IMAGE clone 6063417
Vector Backbone Description: Vector Backbone:pEYFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_63064 Copy
Species: Homo sapiens
Genetic Insert: hspb10
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63111 Copy
Species: Homo sapiens
Genetic Insert: hspb9
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63110 Copy
Species: Homo sapiens
Genetic Insert: expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This construct allows translation of CGG repeats into 99 glycine due to a near cognate ATG codon upstream of the repeat in the 5'UTR of FMR1 (an ACG codon that initiates weakly translation).
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
Proper citation: RRID:Addgene_63089 Copy
Species: Homo sapiens
Genetic Insert: hspb2
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63093 Copy
Species: Homo sapiens
Genetic Insert: hspb3
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63094 Copy
Species: Homo sapiens
Genetic Insert: HOIL-1L
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22863777
Proper citation: RRID:Addgene_63135 Copy
Species: Homo sapiens
Genetic Insert: hspb5
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63096 Copy
Species: Homo sapiens
Genetic Insert: hspb7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63098 Copy
Species: Homo sapiens
Genetic Insert: hspb7
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63108 Copy
Species: Homo sapiens
Genetic Insert: hspb6
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63107 Copy
Species: Homo sapiens
Genetic Insert: hspb10
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63101 Copy
Species: Homo sapiens
Genetic Insert: hspb1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63102 Copy
Species: Homo sapiens
Genetic Insert: hspb2
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20843828
Proper citation: RRID:Addgene_63103 Copy
Species: Homo sapiens
Genetic Insert: Clathrin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mAzurite; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 384; Emission = 450
Proper citation: RRID:Addgene_55228 Copy
Species: Homo sapiens
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Sally Temple; Backbone Size:8000; Vector Backbone:pFUGw (Addgene id: 25870); Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24837679
Comments: Please see http://www.rle.mit.edu/sbg/resources/ for more information.
Proper citation: RRID:Addgene_55195 Copy
Species: Homo sapiens
Genetic Insert: YFP(159-238)-gamma-12
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16641313
Proper citation: RRID:Addgene_55194 Copy
Species: Homo sapiens
Genetic Insert: YFP-(159-238)-gamma-10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16641313
Proper citation: RRID:Addgene_55192 Copy
Species: Homo sapiens
Genetic Insert: CENPB
Vector Backbone Description: Backbone Size:4750; Vector Backbone:EBFP2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 383; Emission = 448
Proper citation: RRID:Addgene_55237 Copy
Species: Homo sapiens
Genetic Insert: Vinculin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mAzurite; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 384; Emission = 450
Proper citation: RRID:Addgene_55234 Copy
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