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Species: Synthetic
Genetic Insert: FnCas12a gRNA targeting TLR 2.0
Vector Backbone Description: Vector Backbone:pBluescript SK (+); Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:36070530
Comments: Please visit https://www.biorxiv.org/content/10.1101/864199v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_117412 Copy
Species: Synthetic
Genetic Insert: EYFP
Vector Backbone Description: Backbone Size:4751; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30626963
Proper citation: RRID:Addgene_117383 Copy
Species: Synthetic
Genetic Insert: BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pICH47751; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson.
Primers to amplify a sgRNA:
Fw: ga GGTCTCa ATTG [x] GTTTAAGAGCTATGCTGGAA, where [x] is the 20nt spacer/target sequence (NOT including the PAM).
Rv: tgtggtctcaAGCGAAAAAAAGCACCGACTC (for polyT terminator)
tgtggtctcaAGCGACCCCAGAAATTGAAC (for 67 bp U6-26 terminator, recommanded)
tgtggtctcaAGCGTATTGGTTTATCTCATCG (for 192 bp U6-26 terminator).
The PCR amplicon is ready to clone in a Golden Gate fashion with U6-26 promoter (pICSL90002) and any Golden Gate compatible acceptor vector Level 1, using BsaI. Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117518 Copy
Species: Synthetic
Genetic Insert: BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pICH47751; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30625150
Comments: Cloned by Laurence Tomlinson.
Primers to amplify a sgRNA:
Fw: ga GGTCTCa ATTG [x] GTTTTAGAGCTAGAAATAGC, where [x] is the 20nt spacer/target sequence (NOT including the PAM).
Rv: tgtggtctcaAGCGAAAAAAAGCACCGACTC.
The PCR amplicon is ready to clone in a Golden Gate fashion with U6-26 promoter (pICSL90002) and any Golden Gate compatible acceptor vector Level 1, using BsaI. Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117517 Copy
Species: Synthetic
Genetic Insert: BsaI:AATG:Cas9_2:GCTT:BsaI
Vector Backbone Description: Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30625150
Comments: Cloned by Oleg Raitskin . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117514 Copy
Species: Synthetic
Genetic Insert: BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pICH47811; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117503 Copy
Species: Synthetic
Genetic Insert: BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pICH47742; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30625150
Comments: Cloned by Baptiste Castel . Please visit https://www.biorxiv.org/content/early/2018/09/17/419952 for bioRxiv preprint.
Proper citation: RRID:Addgene_117502 Copy
Species: Synthetic
Genetic Insert: Promoter_AtU6-26 + sgRNA_AtCas6 {AT5G04770} A. thaliana
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117566 Copy
Species: Synthetic
Genetic Insert: Promoter_AtU6-26 + sgRNA_Methyltransferase {AT5G10620} A. thaliana
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117562 Copy
Species: Synthetic
Genetic Insert: Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117554 Copy
Species: Synthetic
Genetic Insert: Promoter_AtU6-26 + sgRNA_NbPDS1 _1mismatch N. benthamiana
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117555 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: AsCas12a (with stop codon)+3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117550 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: SaCas9 (no stop codon) (pEPOR0SP0012) +c-terminal tag: YFP (pICSL50005) +3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117551 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: SpCas9-p (no stop codon)(pEPOR0SP0009) +c-terminal tag: YFP (pICSL50005)+ 3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117543 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: SpCas9-KA (no stop codon)(pEPOR0SP0015) +c-terminal tag: YFP (pICSL50005)+ 3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117545 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: SpCas9-DE (no stop codon)(pEPOR0SP0001) +c-terminal tag: YFP (pICSL50005)+ 3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117544 Copy
Species: Synthetic
Genetic Insert: crRNA (LbCas12a)
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117541 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 +c-terminal tag: YFP (pICSL50005) +3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117547 Copy
Species: Synthetic
Genetic Insert: 2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Promoter/5'UTR: 2x35S+5'UTR OMEGA (pICH51288) +CDS: LbCas12a (with stop codon) (pEPOR0SP0007)+3'UTR/terminator: Nos (pICH41421) Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117549 Copy
Species: Synthetic
Genetic Insert: sgRNA (Sp Cas9) - extended stem with U626 terminator
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30811422
Comments: Compatible with MoClo, GB, loop Please visit https://www.biorxiv.org/content/early/2018/09/20/422766 for BioRxiv preprint
Proper citation: RRID:Addgene_117538 Copy
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