Plasmids   Select Another Resource Report Type

http://www.addgene.org/72973

Species: Synthetic
Genetic Insert: SJM911 promoter
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3036; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716

Proper citation: RRID:Addgene_72973 Copy   

•   Source: Addgene

http://www.addgene.org/72974

Species: Synthetic
Genetic Insert: SJM912 promoter
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3036; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716

Proper citation: RRID:Addgene_72974 Copy   

•   Source: Addgene

http://www.addgene.org/72971

Species: Synthetic
Genetic Insert: SJM908 promoter
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3036; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716

Proper citation: RRID:Addgene_72971 Copy   

•   Source: Addgene

http://www.addgene.org/72978

Species: Synthetic
Genetic Insert: T7+RBS
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2206; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716

Proper citation: RRID:Addgene_72978 Copy   

•   Source: Addgene

http://www.addgene.org/72979

Species: Synthetic
Genetic Insert: T7+RBS+His6+thrombin
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2264; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716

Proper citation: RRID:Addgene_72979 Copy   

•   Source: Addgene

http://www.addgene.org/73010

Species: Synthetic
Genetic Insert: Secondary module + Golden Gate pTU2a BsmBI destination site
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3120; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27096716

Proper citation: RRID:Addgene_73010 Copy   

•   Source: Addgene

http://www.addgene.org/58495

Genetic Insert: encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Vector Backbone Description: Vector Backbone:pRL542; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:3148842
Comments: MobColK, M.AvaI, M.Eco47II Please see attached map for more information on how this vector was constructed and cloning details.

Proper citation: RRID:Addgene_58495 Copy   

•   Source: Addgene

http://www.addgene.org/70166

Species: alicyclobacillus acidoterrestris
Genetic Insert: AacC2c1 locus
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26593719

Proper citation: RRID:Addgene_70166 Copy   

•   Source: Addgene

http://www.addgene.org/70168

Species: leptotrichia shahii
Genetic Insert: LshC2c2 full locus
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4245; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26593719

Proper citation: RRID:Addgene_70168 Copy   

•   Source: Addgene

http://www.addgene.org/70188

Species: Synthetic
Genetic Insert: amyEopt
Vector Backbone Description: Backbone Marker:Fagan and Fairweather 2009; Backbone Size:7216; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27333161
Comments: Please note: the depositing lab has determined that a particular IS element present in E. coli strains such as DH5alpha and its derivatives has a high propensity to insert into this plasmid. The depositing lab recommends isolating the plasmid DNA and transforming into a strain similar to MC1061. Publication describing the backbone vector pRPF185 as well as the sequencing primers: Clostridium difficile has two parallel and essential Sec secretion systems. Fagan RP, Fairweather NF. J Biol Chem. 2011 Aug 5;286(31):27483-93. doi: 10.1074/jbc.M111.263889. Epub 2011 Jun 9. PMID: 21659510

Proper citation: RRID:Addgene_70188 Copy   

•   Source: Addgene

http://www.addgene.org/70688

Vector Backbone Description: Vector Backbone:see comments; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8412673
Comments: MCS: EcoRV-BglII-XhoI-BssHII-SpeI-BsiWI-AvrII-AflII-BstBI and potentially other sites sacB was derived from pUM24 (Ried, J. L., and A. Colimer. Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921). The polylinker came, in large part, from the plC20R polylinker (Marsh et al.. 1984: Gene 32: 481-485) and a polylinker region of plasmid pJRD184 (Heusterspreute et al.. 1985: Gene 37: 299-304). The erythromycin-resistance gene came from Horinouchi, S., and Weisblum, B. (J. Bacteriol. 150: 804-814, 1982).

Proper citation: RRID:Addgene_70688 Copy   

•   Source: Addgene

http://www.addgene.org/70703

Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus dCas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.

Proper citation: RRID:Addgene_70703 Copy   

•   Source: Addgene

http://www.addgene.org/70702

Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GGGCACGGGCAGCTTGCCGG. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.

Proper citation: RRID:Addgene_70702 Copy   

•   Source: Addgene

http://www.addgene.org/70705

Species: S. aureus
Genetic Insert: mammalian codon-optimized Staphylococcus aureus Cas9
Vector Backbone Description: Backbone Marker:EMD Millipore; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26524662
Comments: For this plasmid, the sequence of the sgRNA spacer is GTCGCCCTCGAACTTCACCT. The length of the sgRNA (not including the spacer sequence) is 84 nucleotides, where this truncated repeat/anti-repeat sgRNA is similar to that described previously by Ran et al., Nature 2015 (PubMed ID: 25830891) and conveys higher activity than the canonical full length sgRNA.

Proper citation: RRID:Addgene_70705 Copy   

•   Source: Addgene

http://www.addgene.org/71207

Species: Synthetic
Genetic Insert: AD1 Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173

Proper citation: RRID:Addgene_71207 Copy   

•   Source: Addgene

http://www.addgene.org/71209

Genetic Insert: pbuE Sense
Vector Backbone Description: Backbone Marker:N/A; Vector Backbone:Custom; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25643173

Proper citation: RRID:Addgene_71209 Copy   

•   Source: Addgene

http://www.addgene.org/71313

Vector Backbone Description: Backbone Size:4378; Vector Backbone:pGK12; Vector Types:Bacterial Expression, E. coli-gram positive bacteria shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11526048
Comments: In plasmid preparations from E. coli, a smaller deletion derivative of pTRK669 of about 1.7 kb may be noticeable. This smaller plasmid does not affect the functionality of pTRK669 in gram positive hosts and may not appear in those backgrounds. Addgene's quality control sequence shows the expected deletion from the smaller derivative of pTRK669. Whole plasmid sequencing from the depositor showed deletion of the NheI site. Please see the provided digest result for reference.

Proper citation: RRID:Addgene_71313 Copy   

•   Source: Addgene

http://www.addgene.org/71420

Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25769287

Proper citation: RRID:Addgene_71420 Copy   

•   Source: Addgene

http://www.addgene.org/71421

Genetic Insert: none
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25769287

Proper citation: RRID:Addgene_71421 Copy   

•   Source: Addgene

http://www.addgene.org/71538

Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pSC101ts; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26141150
Comments: The plasmid curing system consists of an arabinose inducible promoter, PBAD to express a gRNA targeting the bla gene on the gRNA plasmid. When induced, the bla-targeting gRNA is expressed, leading Cas9 to cleave the gRNA plasmid and resulting in plasmid elimination.

Proper citation: RRID:Addgene_71538 Copy   

•   Source: Addgene