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Species: Synthetic
Genetic Insert: U6-Nla repeat-BbsI-Nla repeat
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1812; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178883 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2654; Vector Backbone:BASIC_SEVA_29.10; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178921 Copy
Species: X. albilineans
Genetic Insert: X. albilineans type I-C cas3
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178766 Copy
Species: Synthetic
Genetic Insert: mScarlet counter-selection cassette
Vector Backbone Description: Backbone Size:2729; Vector Backbone:BASIC_SEVA_27.10; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36381610
Comments: Cloning method: BASIC DNA assembly . Please visit https://www.biorxiv.org/content/10.1101/2022.01.06.446575v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_178920 Copy
Species: Synthetic
Genetic Insert: EF1a-NlaCas8c-bGH PolyA
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1993; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178880 Copy
Genetic Insert: EF1a-NlaCas3c-bGH polyA
Vector Backbone Description: Backbone Marker:Lucigen; Backbone Size:1993; Vector Backbone:pSmart HC Kan; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35051351
Proper citation: RRID:Addgene_178881 Copy
Species: S. thermophilus
Genetic Insert: S. thermophilus type I-E cas7
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35216669
Proper citation: RRID:Addgene_178780 Copy
Genetic Insert: Cargo EGFP with AttP Bxb1 site for in-frame fusion
Vector Backbone Description: Vector Backbone:pMC.BESPX; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36424489
Comments: Please visit https://doi.org/10.1101/2021.11.01.466786 for bioRxiv preprint.
Proper citation: RRID:Addgene_179116 Copy
Species: Homo sapiens
Genetic Insert: Src, SsrA
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34637702
Proper citation: RRID:Addgene_179141 Copy
Species: Haemophilus influenzae
Genetic Insert: SspB
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34637702
Proper citation: RRID:Addgene_179142 Copy
Species: Synthetic
Genetic Insert: Sec61b-V5-TurboID
Vector Backbone Description: Backbone Marker:Tong-Chuan He laboratory; Backbone Size:35725; Vector Backbone:pAdTrack-CMV, pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34471136
Comments: Sec61b-V5-TurboID_pcDNA5 is Addgene plasmid # 166971.
To generate adenovirus, digest with PacI and perform transfection to HEK293 cells according to Luo et al (PMID:17546019) procedure #14.
Please note: The plasmid contains a 1650bp insertion just downstream of the Left Arm compared to the depositor's provided sequence. This difference is not known to affect plasmid function.
Proper citation: RRID:Addgene_179143 Copy
Species: Mus musculus
Genetic Insert: kappa opioid receptor
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34162205
Proper citation: RRID:Addgene_179285 Copy
Species: Homo sapiens
Genetic Insert: ELMSAN1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5367; Vector Backbone:pET28C (+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34912035
Comments: The insert encodes the epitope (ELMSAN1 residue 550-600) of the antibody (Catalog # A303-157A, BETHYL). Expressed protein has been confirmed by Western blot.
Proper citation: RRID:Addgene_179391 Copy
Species: A. victoria
Genetic Insert: Ptet gRNA with sfGFP dropout
Vector Backbone Description: Vector Backbone:pMC_V*_01_LVL0* Entry sfGFP (Marburg Collection); Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35338236
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.02.454823v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179340 Copy
Species: A. victoria
Genetic Insert: Ptet gRNA with sfGFP dropout
Vector Backbone Description: Vector Backbone:pMC_V*_01_LVL0* Entry sfGFP (Marburg Collection); Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35338236
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.08.02.454823v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_179336 Copy
Species: Homo sapiens
Genetic Insert: BACE1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22553349
Proper citation: RRID:Addgene_165032 Copy
Species: SARS-COV-2
Genetic Insert: Nsp3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165108 Copy
Species: Homo sapiens
Genetic Insert: TCF4
Vector Backbone Description: Backbone Size:9200; Vector Backbone:pHR; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9065401
Comments: The TCF4 in this construct has a Q27R mutation. It is not known to affect the function.
According to the depositing scientist, other mutations may be present but they have no functional consequences on standard assays for Tcf4 activity.
Proper citation: RRID:Addgene_16514 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: COV E
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFPC1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165123 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165088 Copy
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