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Species: Other
Genetic Insert: loxP-hyg-cat-loxP
Vector Backbone Description: Backbone Marker:Jeffery Murry - Eric Rubin Lab; Vector Backbone:pJM1; Vector Types:Bacterial Expression, Source of loxP-hyg-cat- loxP for PCR amplification.; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25779316
Comments: This vector contains a hyg-cat cassette, flanked by loxP sites, that can be used for the construction of recombineeng substrates for M. smegmatis or M. tuberculosis. It contains a 1 bp change (relative to starting plasmid pJM1) at position 2008 (G to C) that eliminate a potential stop codon that could interfere with expression of downstream functions following excision of the cassette from the chromosome by Cre recombinase.
The hygromycin resistance gene encodes M13I and I111T variants compared to the NCBI reference [ADL66925.1]. These variants should not interfere with function.
Proper citation: RRID:Addgene_71487 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtU6-1 promoter
Vector Backbone Description: Backbone Marker:self-made; derived from the BioBrick assembly plasmid pSB1C3 ; Backbone Size:2105; Vector Backbone:pUPD2; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26839579
Proper citation: RRID:Addgene_75405 Copy
Species: Synthetic
Genetic Insert: His10
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA
Proper citation: RRID:Addgene_55179 Copy
Species: Synthetic
Genetic Insert: cMyc tag
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA
Proper citation: RRID:Addgene_55183 Copy
Species: Methanosarcina barkeri
Genetic Insert: pylS
Vector Backbone Description: Vector Backbone:pSupAR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21892184
Comments: Plasmid for encoding the Methanosarcina barkeri pyrrolysyl-tRNA synthetase (MbPylRS)/tRNA pair in E. coli which can incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into a in-frame amber codon site on a protein of interest.The aminoacyl-tRNA synthetase is engineered to recognize the UAA but not the natural native amino acids, and charge its cognate tRNA with the corresponding UAA. Then, the charged tRNA will insert the UAA at an in-frame amber codon position on a target gene.
Proper citation: RRID:Addgene_91705 Copy
Species: Zea mays
Genetic Insert: HA-ZmSAE1
Vector Backbone Description: Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27208252
Proper citation: RRID:Addgene_91945 Copy
Species: Zea mays
Genetic Insert: ZmSAE2-FLAG
Vector Backbone Description: Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27208252
Proper citation: RRID:Addgene_91943 Copy
Species: Zea mays
Genetic Insert: HA-ZmSAE1
Vector Backbone Description: Backbone Size:4008; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27208252
Proper citation: RRID:Addgene_91944 Copy
Species: leptotrichia shahii
Genetic Insert: LshCas13a locus with non-targeting spacer
Vector Backbone Description: Backbone Size:4153; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28408723
Comments: Note: Addgene's quality control sequencing identified an R820W amino acid residue substitution in the LshCas13a insert. This residue change is not thought to affect plasmid function.
Proper citation: RRID:Addgene_91901 Copy
Species: Rhodobacter capsulatus R121
Genetic Insert: RcrCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91921 Copy
Species: Rhodobacter capsulatus DE442
Genetic Insert: RcdCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91922 Copy
Species: Rhodobacter capsulatus SB 1003
Genetic Insert: RcsCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91920 Copy
Species: Listeriaceae bacterium FSL M6-0635
Genetic Insert: LbfCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4196; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Comments: Note: Addgene's quality control sequencing suggests that there may be a mixed population of LbfCas13a Ala727 and Thr727.
Proper citation: RRID:Addgene_91918 Copy
Species: Listeria seeligeri
Genetic Insert: LseCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91910 Copy
Species: Lachnospiraceae bacterium MA2020
Genetic Insert: LbmCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91911 Copy
Species: Leptotrichia wadei
Genetic Insert: LwCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91909 Copy
Species: Leptotrichia shahii
Genetic Insert: LshCas13a and guide expression cassette
Vector Backbone Description: Backbone Size:4197; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28976959
Proper citation: RRID:Addgene_91908 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:8144; Vector Backbone:pKT230; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59772 Copy
Species: Other
Genetic Insert: Ham1
Vector Backbone Description: Vector Backbone:pSALECT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25790188
Proper citation: RRID:Addgene_59706 Copy
Species: Mus musculus
Genetic Insert: SupH
Vector Backbone Description: Vector Backbone:pSALECT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25790188
Proper citation: RRID:Addgene_59710 Copy
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