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Species: Mus musculus
Genetic Insert: Siah2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_122041 Copy
Species: Mus musculus
Genetic Insert: Smarca4
Vector Backbone Description: Backbone Marker:Carmen Williams; Backbone Size:3059; Vector Backbone:pIVT2; Vector Types:For in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29325037
Proper citation: RRID:Addgene_122146 Copy
Species: Mus musculus
Genetic Insert: Myocardin
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pShuttle; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19342595
Comments: Myocardin contains an A435T difference compared to the Myocd NCBI entry. The functional consequence of this difference is unknown.
Proper citation: RRID:Addgene_122122 Copy
Species: Mus musculus
Genetic Insert: sgRNA for mouse Nanog
Vector Backbone Description: Backbone Size:8427; Vector Backbone:px335; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32596448
Proper citation: RRID:Addgene_122303 Copy
Species: Mus musculus
Genetic Insert: Hmga1a
Vector Backbone Description: Backbone Marker:Yukiko Gotoh, Addgene plasmid #122292; Vector Backbone:pCAG2IG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22797695
Comments: Please note: Addgene NGS unable to fully resolve CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_122293 Copy
Species: Mus musculus
Genetic Insert: Hmga2
Vector Backbone Description: Backbone Marker:Yukiko Gotoh, Addgene plasmid #122292; Vector Backbone:pCAG2IG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22797695
Comments: Please note: Addgene NGS unable to fully resolve CAG promoter sequence. Please refer to the depositor's sequence for this section of the plasmid.
Proper citation: RRID:Addgene_122294 Copy
Species: Mus musculus
Genetic Insert: reelin
Vector Backbone Description: Backbone Marker:Curran lab (Addgene plasmid 122443); Vector Backbone:pCrl; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8987733
Proper citation: RRID:Addgene_122444 Copy
Species: Mus musculus
Genetic Insert: reelin
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8987733
Comments: The entire reelin open reading frame was assembled by fusing five overlapping cDNA clones isolated previously (D’Arcangelo et al., 1995) and subcloning them into pcDNA3. The following reelin fragments were used: 1.2 kb NaeI–SalI from p59BS1, 1.3 kb SalI–NdeI from pBS2, 3.1 kb NdeI–BspEI from pBS6, 770 bp BspEI–ApaLI from p3Rea3, and 4.2 kb ApaLIEcoRV from pBS53. The final clone (pCrl) contains the entire reelin open reading frame (10,383 bp) plus 95 bp of sequence 59 to the initiator methionine codon and 82 bp of 39 untranslated sequence (reelin cDNA nucleotides 188–10,748).
Proper citation: RRID:Addgene_122443 Copy
Species: Mus musculus
Genetic Insert: Mus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122673 Copy
Species: Mus musculus
Genetic Insert: proprotein convertase subtilisin/kexin type 1 (PCSK1)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122672 Copy
Species: Mus musculus
Genetic Insert: Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4)
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122675 Copy
Species: Mus musculus
Genetic Insert: Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1/V5-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30104250
Proper citation: RRID:Addgene_122681 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations A74V, M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12290 Copy
Species: Mus musculus
Genetic Insert: m(KS_MAT)_mouseL_ACP
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122850 Copy
Species: Mus musculus
Genetic Insert: mouse FASN/ mouse fatty acid synthase
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122847 Copy
Species: Mus musculus
Genetic Insert: m(KS_MAT)
Vector Backbone Description: Backbone Marker:Novagen/Merck Millipore; Backbone Size:5340; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30394635
Proper citation: RRID:Addgene_122849 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12291 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12293 Copy
Species: Mus musculus
Genetic Insert: TLR2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFlag-CMV-1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16832055
Comments: Murine TLR2 expression vector was made by removing the original signal peptide and replacing it with the signal peptide from preprotrypsin. When compared to GenBank reference NP_036035.3, the TLR2 insert in this plasmid appears to be missing aa1-23 due to the removal of the endogenous TLR2 signal peptide. See associated publication for more information.
Also, please note that Addgene's sequencing results identified additional mutations M82I and H202Y when compared to GenBank reference NP_036035.3. M82I and H202Y are possible polymorphisms as they were also present in the wild-type TLR2 sequence from the depositing laboratory (Addgene Plasmid 12291: pCMV-M2-TLR2). It is not known whether these mutations affect TLR2 function.
Proper citation: RRID:Addgene_12292 Copy
Species: Mus musculus
Genetic Insert: PSAM4 5HT3 LC IRES GFP
Vector Backbone Description: Backbone Size:6600; Vector Backbone:pCAG IRES eGFP WPRE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30872534
Comments: Please note that there are ambiguous bases in the CAG promoter due to difficulties sequencing through GC-rich regions.
Proper citation: RRID:Addgene_123066 Copy
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