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Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32315611
Proper citation: RRID:Addgene_155264 Copy
Species: Rattus norvegicus
Genetic Insert: F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32527825
Proper citation: RRID:Addgene_155217 Copy
Species: Rattus norvegicus
Genetic Insert: p190 GTPase activating protein
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9852136
Comments: Digesting the plasmid with BamHI+EcoRI will excise the entire full-length p190 ORF.
Proper citation: RRID:Addgene_15547 Copy
Species: Rattus norvegicus
Genetic Insert: PHAS-I
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4671; Vector Backbone:pET14b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12665511
Comments: Full-length PHASI with a His tag. See author's map for detailed information.
Proper citation: RRID:Addgene_15679 Copy
Species: Rattus norvegicus
Genetic Insert: rApoI
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:29991261
Comments: Esherichia coli strain that expresses a deactivated variant of MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth drApo1-T7]
Proper citation: RRID:Addgene_156461 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33097540
Comments: Existing fluorescent protein in plasmid Adggene# 56618 was replaced with mGold.
"N-20" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
Proper citation: RRID:Addgene_158006 Copy
Species: Rattus norvegicus
Genetic Insert: Zdk2, Cofilin, LOV2
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31740825
Comments: There is a point mutation in the Zdk portion of the dark control construct. In our hands, this had no effect on the experiments, and it is a position where it would not be expected to impact binding or optogenetic response.
Proper citation: RRID:Addgene_141135 Copy
Species: Rattus norvegicus
Genetic Insert: OMP25
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4692; Vector Backbone:pAcGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32328629
Proper citation: RRID:Addgene_141150 Copy
Species: Rattus norvegicus
Genetic Insert: OMP25
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4689; Vector Backbone:pAcGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32328629
Proper citation: RRID:Addgene_141151 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9667; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139659 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9263; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139662 Copy
Species: Rattus norvegicus
Genetic Insert: AMPK-Kinase-dead
Vector Backbone Description: Vector Backbone:pLenti-X1-Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160526
Proper citation: RRID:Addgene_139844 Copy
Species: Rattus norvegicus
Genetic Insert: BE4max(R33A)_NG-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140257 Copy
Species: Rattus norvegicus
Genetic Insert: BE4max(R33A)
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140251 Copy
Species: Rattus norvegicus
Genetic Insert: BE4max(R33A)-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140253 Copy
Species: Rattus norvegicus
Genetic Insert: Rat Fibronectin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGEM-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6317187
Proper citation: RRID:Addgene_14057 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT1
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3200; Vector Backbone:pBS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3016720
Proper citation: RRID:Addgene_15993 Copy
Species: Rattus norvegicus
Genetic Insert: AMPK alpha2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11389854
Proper citation: RRID:Addgene_15991 Copy
Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: Targets all collybistin mRNAs (target is located in the protein coding region. Nucleotides 1316-1335 of AJ302676 [CB2SH3-] or 1496-1515 of AJ250425 [CB1SH3+]. Targets all collybistin isoforms).
Proper citation: RRID:Addgene_160065 Copy
Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: 3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain
Proper citation: RRID:Addgene_160067 Copy
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