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http://www.addgene.org/160068

Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: Targets collybistin SH3+ mRNAs (target is located in the protein coding region of SH3+. Nucleotides 498-518 of AJ250425 [CB1SH3+]. Targets collybistin isoforms with SH3 domain).

Proper citation: RRID:Addgene_160068 Copy   

•   Source: Addgene

http://www.addgene.org/160069

Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Backbone Marker:Modified from Bryan Roth (Addgene plasmid # 65417); Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34083383

Proper citation: RRID:Addgene_160069 Copy   

•   Source: Addgene

http://www.addgene.org/160213

Species: Rattus norvegicus
Genetic Insert: ARHGEF9
Vector Backbone Description: Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33316079
Comments: 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Addgene Plasmid #160212).

Proper citation: RRID:Addgene_160213 Copy   

•   Source: Addgene

http://www.addgene.org/16077

Species: Rattus norvegicus
Genetic Insert: soluble adenylyl cyclase
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9874775
Comments: Original library clone from Lambda-Zap rat testis library. CTCT repeats inserted between EcoRI sites in 5' UTR. Clone starts at nucleotide 241 of sAC sequence. EcoRI/XhoI digest: 0.7, 1.1, 1.5, 2.0, 4.5 kb. pSAC5.02 is derived from an original library clone obtained in the cloning of rat sAC. The isolation of this cDNA clone seems outdated now, but it was still a current method back in 1999, when we cloned sAC. We obtained peptide sequences from purified sAC protein, and designed PCR primers to those peptides. These resulted in isolation of a 1 kb fragment encoding a portion of the sAC cDNA, but there was open reading frame on each end of this PCRd clone. We then screened a rat testis library we generated in the lamdba zap cloning system. We screened the library by hybridization using the 1 kb PCR fragment as probe and we identified three, independent cDNA clones. THe lambda zap cloning system that we used enabled isolation of plasmids from the lambda phage library using helper phage. THe resultant plasmids contained library inserts (in our case, a sAC cDNA) in the pBK-CMV cloning vector. In this cloning vector, the cDNAs were inserted directionally, meaning that the vectors were already suitable for bacterial expression using the T7 promoter. We were unable to get sufficient protein via bacterial expression, so we deleted the T7 promoter region by digesting with NheI-SpeI and reclosing the vector; this brought the CMV promoter closer to the inserted sAC cDNA and facilitated high level expression in mammalian cells (i.e., HEK293 cells). This is how plasmid psAC5.02 was generated. THe CTCT repeats are within the 5' UTR of the sAC cDNA - they may have been a cloning artifact during generation of the library, or they may represent some 5' control element. The cDNA clone can not be moved (in its entirety) as an EcoR1 fragment (the pBK-CMV vector has EcoRI sites flanking the inserted cDNA) because there is an internal EcoR1 site within the sAC cDNA. EcoR1 can be used as a diagnostic digest to confirm the validity of the plasmid.

Proper citation: RRID:Addgene_16077 Copy   

•   Source: Addgene

http://www.addgene.org/17287

Species: Rattus norvegicus
Genetic Insert: PV-DsRed-NLS-CD
Vector Backbone Description: Backbone Size:33400; Vector Backbone:Adenoviral Recombinant (pAdEasy); Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17420246
Comments: AdEasy is a registered trademark of the Johns Hopkins University.

Proper citation: RRID:Addgene_17287 Copy   

•   Source: Addgene

http://www.addgene.org/17300

Species: Rattus norvegicus
Genetic Insert: PV-GFP-NLS
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pCMV-Myc-Cyto-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11971908

Proper citation: RRID:Addgene_17300 Copy   

•   Source: Addgene

http://www.addgene.org/173008

Species: Rattus norvegicus
Genetic Insert: DHFR-GFP-th-GluA1
Vector Backbone Description: Backbone Size:8420; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34241635
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.09.02.278770v1 for bioRxiv preprint.

Proper citation: RRID:Addgene_173008 Copy   

•   Source: Addgene

http://www.addgene.org/173159

Species: Rattus norvegicus
Genetic Insert: jGCaMP7c variant 1513-WPRE
Vector Backbone Description: Backbone Size:4764; Vector Backbone:pAAV-GFAP-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_173159 Copy   

•   Source: Addgene

http://www.addgene.org/17385

Species: Rattus norvegicus
Genetic Insert: FEZ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCDNA4 TO2 myc HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15879557
Comments: Full-length Rat2 FEZ1 sequences, 1179 bp, was amplified using cDNA from the wild-type Rat2 fibroblasts or the mutant R3-2 line as templates. The sequences of the PCR primers for generation of C-terminal myc-His-tagged FEZ1 were as follows: forward primer, FEZ1-S1-BamHI, 5'-GCTGATGGATCCACCATGGAGGCTC CACTGGTGAGT3'; reverse primer, FEZ1-A3-XhoI, 5'-GCAACGCTCGAGCAGGTTGGACAGAGCACTTTTAAA-3'.

Proper citation: RRID:Addgene_17385 Copy   

•   Source: Addgene

http://www.addgene.org/17928

Species: Rattus norvegicus
Genetic Insert: N-methyl-D-aspartate receptor NMDAR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11897109

Proper citation: RRID:Addgene_17928 Copy   

•   Source: Addgene

http://www.addgene.org/167970

Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4682; Vector Backbone:pmRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28320970

Proper citation: RRID:Addgene_167970 Copy   

•   Source: Addgene

http://www.addgene.org/16798

Species: Rattus norvegicus
Genetic Insert: Rfz1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96. Rfz1 from Henk Roelink. This sequence has a few differences from the published sequence. Sites listed in Rfz1 are not a complete list. MYC tag version made by Jeff Brown (2/98-3/98), inserted the epitope into the StyI site (1904). The MYC epitope has a BglII site in it. The 3' Sty site is destroyed by the tags, but the 5' site is intact. Epitope can be detected in PBS/Formaldehyde fixed tissue permeabilized with 0.2% Triton. The Myc tagged Rfz1 has been IP'd from a TBS/1% Triton buffer. Frizzled tends to ppt out of SDS sample buffer when boiled, so either warm samples to ~70 degrees Celsius for a few minutes before loading samples or use a strong denaturing loading buffer and incubate the samples at room temp for half an hour before loading. The StyI site is in the 3rd extracellular loop of the protein.

Proper citation: RRID:Addgene_16798 Copy   

•   Source: Addgene

http://www.addgene.org/16796

Species: Rattus norvegicus
Genetic Insert: Rfz2 TG2myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Jeff Brown (tags), 11/23/98. A single c-myc epitope has been inserted into the third extracellular loop of Rfz2 by introduction of an NheI site and the epitope. The epitope was inserted in the region of the loop where the myc and the HA tags were inserted into the Rfz1 tagged constructs--3 aa's after the PCP sequence--which appear to be functional. *The ClaI and XbaI sites are methylated and cannot be cut unless the construct is grown up in dam-bugs.

Proper citation: RRID:Addgene_16796 Copy   

•   Source: Addgene

http://www.addgene.org/16795

Species: Rattus norvegicus
Genetic Insert: beta 2 AR Fz1
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3.1; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588714
Comments: Chimera of beta 2 adrenergic receptor (extracellular and. transmembrane domains) and rat frizzled-1 (intracellular). Activated by isoproterenol and inhibited by propranolol. Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al., 2000, JBC 275:12701-12711.

Proper citation: RRID:Addgene_16795 Copy   

•   Source: Addgene

http://www.addgene.org/16814

Species: Rattus norvegicus
Genetic Insert: rat frizzled 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Insert size: ~1912 bp (EcoRI/Xba I both blunted) Transcription: linearize with Asp 718 and transcribe with SP6. Plasmid History: Constructed by Julia Yang-Snyder on the date of 5/19/96. Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992. Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.

Proper citation: RRID:Addgene_16814 Copy   

•   Source: Addgene

http://www.addgene.org/16821

Species: Rattus norvegicus
Genetic Insert: rat frizzled 1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I or Asp 718 and transcribe with SP6. Plasmid History: Constructed by Julia Yang-Snyder on the date of 4/29/96. Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992. Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.

Proper citation: RRID:Addgene_16821 Copy   

•   Source: Addgene

http://www.addgene.org/16820

Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter. Reference: The insert was published by Wang et al., JBC 271: 4468 (1996). This unpublished construct was generated by Julia Yang-Snyder.

Proper citation: RRID:Addgene_16820 Copy   

•   Source: Addgene

http://www.addgene.org/168513

Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23791179

Proper citation: RRID:Addgene_168513 Copy   

•   Source: Addgene

http://www.addgene.org/169003

Species: Rattus norvegicus
Genetic Insert: rat LAMP1-EGFP
Vector Backbone Description: Backbone Size:4125; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33296658

Proper citation: RRID:Addgene_169003 Copy   

•   Source: Addgene

http://www.addgene.org/169366

Species: Rattus norvegicus
Genetic Insert: Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
Vector Backbone Description: Vector Backbone:pETDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23775070

Proper citation: RRID:Addgene_169366 Copy   

•   Source: Addgene