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Vector Backbone Description: Backbone Marker:Theodora Agalioti; Vector Backbone:Migr1; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28508873
Comments: This construct was created in G. Stathopoulos lab in Patras, Greece, by Theodora Agalioti; The backbone is derived from MigR1 vector to which a partial IRES followed by puromycin selection gene derived from pPY_CAG_CRE_IP plasmid was inserted
Proper citation: RRID:Addgene_64335 Copy
Species: Mus musculus
Genetic Insert: Rab7
Vector Backbone Description: Backbone Size:6280; Vector Backbone:pBa-FRB-3myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25624392
Comments: pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
Proper citation: RRID:Addgene_64210 Copy
Species: Synthetic
Genetic Insert: OmpA_R4cons_G125L
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Proper citation: RRID:Addgene_64298 Copy
Species: Synthetic
Genetic Insert: gBlock product of trp1 deletion gRNA cassette
Vector Backbone Description: Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25281382
Comments: Note: Addgene's quality control sequencing finds one mismatch in the SNR52 promoter region, but plasmid function is not impacted.
Proper citation: RRID:Addgene_64331 Copy
Species: Synthetic
Genetic Insert: OmpA_H1358
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqQgIgAfAgIqVnpyVgFeMgYdWlgrmpykgsvengaykaqgYqAtLkMgVpitddLdIyTrLgGmVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64297 Copy
Species: Synthetic
Genetic Insert: gBlock product of ura3 deletion gRNA cassette
Vector Backbone Description: Backbone Size:6217; Vector Backbone:pRS42H; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25281382
Proper citation: RRID:Addgene_64330 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64324); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter.
Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.
Proper citation: RRID:Addgene_64221 Copy
Species: Streptococcus pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64323); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-BFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25803306
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter.
Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.
Proper citation: RRID:Addgene_64220 Copy
Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11101; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25721206
Comments: The transgene of interest can be inserted into BamHI, PmeI, and PstI sites. However, only the PmeI and PstI sites are recommended to be used for different restriction end ligation.
This plasmid must be used together with an independent tTA-expressing plasmid for Tet-Off system or rtTA-expressing plasmid for Tet-On system.
The TetO promoter sequence was excised from pETE-Bsd plasmid vector, which was originated from pUHD-10-3. The pETE-Bsd and pUHD-10-13 were engineered by Protopopov et al. and Damke et al, respectively.
Protopopov AI, Li J, Winberg G, Gizatullin RZ, Kashuba VI, Klein G, et al. Human cell lines engineered for tetracycline-regulated expression of tumor suppressor candidate genes from a frequently affected chromosomal region, 3p21. J Gene Med. 2002/07/19 ed. 2002;4(4):397–406.
Damke, H., Gossen, M., Freundlieb, S., Bujard, H., and Schmid, S.L. (1995). Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells. Methods Enzymol. 257, 209–220.
Proper citation: RRID:Addgene_64238 Copy
Species: Homo sapiens
Genetic Insert: miR146a
Vector Backbone Description: Backbone Marker:Scott Lowe; Backbone Size:7658; Vector Backbone:MSCV PIG; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18066065
Proper citation: RRID:Addgene_64234 Copy
Species: Synthetic
Genetic Insert: OmpA_R4cons_G99I
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Proper citation: RRID:Addgene_64300 Copy
Vector Backbone Description: Backbone Marker:Richard Morgan NCI; Backbone Size:6700; Vector Backbone:pMSGV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25757546
Proper citation: RRID:Addgene_64269 Copy
Species: Synthetic
Genetic Insert: OmpA_R4cons_P121F
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Proper citation: RRID:Addgene_64301 Copy
Species: Homo sapiens
Genetic Insert: COL11A2 promoter
Vector Backbone Description: Vector Backbone:pLe6Δ (P4-32); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24146984
Proper citation: RRID:Addgene_64314 Copy
Vector Backbone Description: Backbone Marker:Richard Morgan NCI; Backbone Size:8070; Vector Backbone:pMSGV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25757546
Proper citation: RRID:Addgene_64272 Copy
Vector Backbone Description: Backbone Marker:Richard Morgan NCI; Backbone Size:6700; Vector Backbone:pMSGV; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25757546
Proper citation: RRID:Addgene_64271 Copy
Species: Synthetic
Genetic Insert: OmpA_H-46
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgFeMgYdWlgrmpykgsvengaykaqgYqAtLkMgVpitddLdIyTrLgGmVwradtksnvygknhdtgvsFvMaLgSeHaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64311 Copy
Species: Synthetic
Genetic Insert: Infrared fluorescent tobacco etch virus protease sensor (iTEV)
Vector Backbone Description: Backbone Size:5360; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733847
Proper citation: RRID:Addgene_64277 Copy
Species: Synthetic
Genetic Insert: OmpA_H-3
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgLeLgVdVlgrmpykgsvengaykaqgVqLtAkLgYpitddLdIyTrLgGmVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaTrLeYqWtnnigdYPYDVPDYAtrpdnGmLsLgVsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64310 Copy
Species: Synthetic
Genetic Insert: OmpA_H-78
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgFeMgYdWlgrmpykgsvengaykaqgVqLtAkLgYpitddLdIyTrLgGmVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64313 Copy
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