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Species: S. mediterranea (planarian)
Genetic Insert: npy-10
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26473 Copy
Species: S. mediterranea (planarian)
Genetic Insert: ppp-2
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26470 Copy
Species: S. mediterranea (planarian)
Genetic Insert: mpl-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26472 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: response regulator in two-component regulatory system with EvgS
Vector Backbone Description: Backbone Size:3851; Vector Backbone:pDRIVE-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: TA cloning product with 286 bp 5'-UTR including EvgA binding site.
Proper citation: RRID:Addgene_72467 Copy
Species: Acetobacter aceti 1023
Genetic Insert: DNA mismatch repair protein MutS (partial gene)
Vector Backbone Description: Backbone Size:3851; Vector Backbone:pDRIVE-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Used for nucleotide quantitation in RT-PCR and similar experiments for Acetobacter aceti 1023.
Based on method described in M. Ishikawa, A. Okamoto-Kainuma, T. Jochi, I. Suzuki, K. Matsui, T Kaga, Y. Koizumi (2009) Cloning and characterization of grpE in Acetobacter pasteurianus NBRC 3283. J. Biosci. Bioeng. 109(1), 25–31[doi: 10.1016/j.jbiosc.2009.07.008]
Proper citation: RRID:Addgene_72437 Copy
Species: Gallus gallus
Genetic Insert: CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4339; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24685175
Proper citation: RRID:Addgene_69138 Copy
Species: S. pyogenes
Genetic Insert: Cas9 nickase
Vector Backbone Description: Backbone Marker:Thermo-Fisher; Backbone Size:3900; Vector Backbone:PCR2-1 TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
Proper citation: RRID:Addgene_71311 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71739 Copy
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71736 Copy
Species: Synthetic
Genetic Insert: Staphylococcus aureus guide RNA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_64710 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68029 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68033 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68032 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68031 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68030 Copy
Species: Escherichia coli W
Genetic Insert: cscAKB
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4059; Vector Backbone:pCR2.1; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21907272
Comments: The transferrable sucrose utilization cassette contains cscA (invertase), cscK (d-fructokinase), and cscB (sucrose permease).
Proper citation: RRID:Addgene_63918 Copy
Species: Synthetic
Genetic Insert: CasRx
Vector Backbone Description: Vector Backbone:PB; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32185621
Comments: INSERT: CasRx & EGFP driven by CAG promoter.
Proper citation: RRID:Addgene_154004 Copy
Species: Homo sapiens
Genetic Insert: FMR1-FLAG
Vector Backbone Description: Backbone Size:8012; Vector Backbone:Addgene #31938; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32179589
Proper citation: RRID:Addgene_157784 Copy
Species: Homo sapiens
Genetic Insert: Fragment of human 53BP1
Vector Backbone Description: Vector Backbone:mouse deltaPE Oct4-GFP transgenic reporter (addgene plasmid #52382); Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32343484
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.29.925487v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141122 Copy
Species: Synthetic
Genetic Insert: dLbCas12a(D156R)-VPR
Vector Backbone Description: Backbone Marker:DOI: 10.1039/C8SC02870B; Backbone Size:14777; Vector Backbone:pYFAC-riboB; Vector Types:CRISPR, Synthetic Biology, Expression in Aspergillus nidulans and related filamentous fungi.; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32526136
Proper citation: RRID:Addgene_140198 Copy
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