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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pMiR-CIP2A-miR-375-mutE Resource Report Resource Website |
RRID:Addgene_53710 | CIP2A | Homo sapiens | Kanamycin | PMID:23552692 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | Mutation on miR-375 binding site E (refer to citation) | 2026-08-15 01:16:33 | 0 | |
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IL10RB_pCSdest Resource Report Resource Website |
RRID:Addgene_53799 | IL10RB | Homo sapiens | Ampicillin | PMID:29760202 | NM_000628 | Backbone Marker:Lawson Lab (Addgene Plasmid 22423); Backbone Size:4095; Vector Backbone:pCSDest; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 0 | |
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CDKL5A-c014 Resource Report Resource Website |
RRID:Addgene_53686 | CDKL5A | Other | Ampicillin | Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/4BGQ/ | Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin | Activating mutations T169D / Y171E | 2026-08-15 01:16:33 | 0 | |
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DDR1A-c002 Resource Report Resource Website |
RRID:Addgene_53687 | DDR1A | Ampicillin | Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/3ZOS/ | Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 0 | |||
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SOCS2A-c016 Resource Report Resource Website |
RRID:Addgene_53689 | SOCS2A | Other | Ampicillin | T7-lacl regulated, N-terminal his tag, TEV cleavage, LIC cloning using BseRI/ T4 pol, pET backbone. http://www.thesgc.org/structures/2C9W/ (complex) | Backbone Marker:SGC; Vector Backbone:pLIC-SGC1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Bacterial expression for structure determination; may not be full ORF, see comments | 2026-08-15 01:16:33 | 0 | |
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BMPR1BA-c017 Resource Report Resource Website |
RRID:Addgene_53690 | BMPR1BA | Ampicillin | Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/3MDY/ | Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 0 | |||
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pMiR-HPV16-E7-m2 Resource Report Resource Website |
RRID:Addgene_53698 | HPV16-E7 | Human papillomavirus | Kanamycin | PMID:24708873 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | Mutation in miR-375 binding site m2 (refer to citation) | 2026-08-15 01:16:33 | 0 | |
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pBRα Resource Report Resource Website 1+ mentions |
RRID:Addgene_53731 | αNTD | E. coli | Ampicillin | PMID:9121589 | This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 1 | |
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pMiR-E6AP-3'UTR-miR-375-mut Resource Report Resource Website |
RRID:Addgene_53695 | UBE3A 3'UTR | Homo sapiens | Kanamycin | PMID:24708873 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | Mutation on miR-375 binding site on E6AP 3'UTR (refer to citation) | 2026-08-15 01:16:33 | 0 | |
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pMiR-HPV16-E7 Resource Report Resource Website 1+ mentions |
RRID:Addgene_53696 | HPV16-E7 | Human papillomavirus | Kanamycin | PMID:24708873 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:33 | 1 | ||
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ACVR1A-c076 Resource Report Resource Website |
RRID:Addgene_53691 | ACVR1A | Ampicillin | Baculovirus transfer vector (Bac-to-bac), N-terminal His-tag, TEV http://www.thesgc.org/structures/3H9R/ | Backbone Marker:SGC; Vector Backbone:pFB-LIC-Bse; Vector Types:Baculovirus expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:34 | 0 | |||
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pDESTN-MTp-p53-Venus Resource Report Resource Website |
RRID:Addgene_53736 | p53 | Homo sapiens | Ampicillin | PMID:18471974 | Backbone Marker:Gateway; Vector Backbone:pDEST R4-R3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:34 | 0 | ||
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FW102 OL2-62 Resource Report Resource Website |
RRID:Addgene_53735 | none | E.coli | Kanamycin and Streptomycin | PMID:9121589 | FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription. This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article: http://www.addgene.org/browse/article/8393/ This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310 | Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin and Streptomycin | 2026-08-15 01:16:33 | 0 | |
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pMiR-HPV16-E1(partial) Resource Report Resource Website 1+ mentions |
RRID:Addgene_53699 | HPV16-E1(partial) | Human papillomavirus | Kanamycin | PMID:24708873 | Backbone Marker:Origene; Backbone Size:7900; Vector Backbone:pMiR-Target; Vector Types:Luciferase; Bacterial Resistance:Kanamycin | 2026-08-15 01:16:33 | 1 | ||
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pACλCI-β-flap (831-1057) Resource Report Resource Website 1+ mentions |
RRID:Addgene_53733 | λCI-β-flap fusion | Other | Chloramphenicol | PMID:9121589 | Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details. This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642 | Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:16:34 | 1 | |
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pcDNA3/Flag-WTAP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53741 | Wilms tumor 1 associated protein | Homo sapiens | Ampicillin | PMID:24316715 | Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 8 | ||
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pCS-membrane-ceruleanFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53749 | 2x membrane linker | Other | Ampicillin | PMID:19378112 | Use NotI/SP6 for RNA to inject. Put XbaI/AgeI membrane linker in place of histone2B in XbaI/AgeI cut pCS-H2Bcerulean. This puts membrane-cerulean between XbaI and SnaBI of pCS. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 2 | |
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pCS-H2B-EYFP Resource Report Resource Website |
RRID:Addgene_53746 | histone H2B | Homo sapiens | Ampicillin | PMID:19378112 | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:34 | 0 | ||
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pCS-H2B-EGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_53744 | histone H2B | Homo sapiens | Ampicillin | PMID:19378112 | Cut with NotI and use SP6 for sense RNA. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 6 | |
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pCS-H2B-citrine Resource Report Resource Website |
RRID:Addgene_53752 | histone H2B | Homo sapiens | Ampicillin | PMID:19378112 | This plasmid was created using the QuikChange protocol to perform site directed mutagenesis to make Q69M mutation to convert EYFP of pCS-H2B-EYFP (Addgene plasmid # 53746) into citrine. Use NotI/SP6 for transcription. | Backbone Marker:RZPD; Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:33 | 0 |
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