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Species: Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert)
Genetic Insert: BsMDH BmHPS BmPHI (on Addgene plasmid # 129104)
Vector Backbone Description: Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:33404828
Comments: This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH.
This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis.
Proper citation: RRID:Addgene_159446 Copy
Genetic Insert: cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
Vector Backbone Description: Vector Backbone:pMW118; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176232 Copy
Genetic Insert: cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
Vector Backbone Description: Vector Backbone:pMW118; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176231 Copy
Genetic Insert: cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
Vector Backbone Description: Vector Backbone:pMW118; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176233 Copy
Genetic Insert: cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
Vector Backbone Description: Vector Backbone:pMW118; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176230 Copy
Genetic Insert: cI of phage lambda, hok of the R1 plasmid, neo
Vector Backbone Description: Vector Backbone:pMW118; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35920321
Proper citation: RRID:Addgene_176227 Copy
Species: Homo sapiens
Genetic Insert: STAT5B
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176339 Copy
Species: Homo sapiens
Genetic Insert: HDX
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176353 Copy
Species: Homo sapiens
Genetic Insert: RORB
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176354 Copy
Species: Homo sapiens
Genetic Insert: BACH2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: SK-N-SH cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176357 Copy
Species: Homo sapiens
Genetic Insert: OTX2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176358 Copy
Species: Homo sapiens
Genetic Insert: ZNF22
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176349 Copy
Species: Homo sapiens
Genetic Insert: SNAPC5
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176341 Copy
Species: Homo sapiens
Genetic Insert: ZBED9
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176344 Copy
Species: Homo sapiens
Genetic Insert: CHCHD3
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176347 Copy
Species: Homo sapiens
Genetic Insert: ZBTB4
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176374 Copy
Species: Homo sapiens
Genetic Insert: TBX18
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176370 Copy
Species: Homo sapiens
Genetic Insert: PRDM15
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: PGP1 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176373 Copy
Species: Homo sapiens
Genetic Insert: PITX2
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: A549 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176372 Copy
Species: Homo sapiens
Genetic Insert: TRERF1
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
Proper citation: RRID:Addgene_176366 Copy
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