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Species: codon optimized SARS-COV-2
Genetic Insert: Nsp13
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165136 Copy
Species: codon optimized SARS-COV-2
Genetic Insert: Nsp14
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33666950
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.19.423586v1 for BioRxiv preprint. bioRxiv 2020.12.19.423586v1
Proper citation: RRID:Addgene_165137 Copy
Species: Influenza A virus
Genetic Insert: Influenza B virus(B/Georgia/05/2019) nucleocapsid protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165093 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173)
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165091 Copy
Species: Influenza A virus
Genetic Insert: Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165092 Copy
Species: SARS-CoV2
Genetic Insert: Severe acute respiratory syndrome coronavirus 2 spike protein
Vector Backbone Description: Vector Backbone:pET-30a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33532799
Proper citation: RRID:Addgene_165096 Copy
Species: Synthetic
Genetic Insert: ABE8e-NRCH
Vector Backbone Description: Backbone Marker:Atum; Backbone Size:2253; Vector Backbone:pD881-SR; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_165417 Copy
Genetic Insert: hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
Vector Backbone Description: Vector Backbone:pGHUC_R25; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33441553
Proper citation: RRID:Addgene_165604 Copy
Genetic Insert: hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
Vector Backbone Description: Backbone Marker:Marcus Noyes Lab (Addgene #165604); Vector Backbone:pGG184; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33441553
Proper citation: RRID:Addgene_165605 Copy
Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10490837
Comments: RKO (colorectal cancer cell line) mutation: 4 base-pair deletion (AGGCAGG to AGG) within the sequence corresponding to helix 3 of the CDX2 homeodomain.
Proper citation: RRID:Addgene_16554 Copy
Species: Homo sapiens
Genetic Insert: CDX2
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10490837
Proper citation: RRID:Addgene_16553 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:7462; Vector Backbone:pHRCMV; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:9659898
Proper citation: RRID:Addgene_16552 Copy
Vector Backbone Description: Backbone Size:1941; Vector Backbone:pR6Kg; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha1
Proper citation: RRID:Addgene_165862 Copy
Species: Homo sapiens
Genetic Insert: cAPC
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:0; Vector Backbone:pShuttle / pAdTrack ?; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:10749138
Comments: The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites.
Proper citation: RRID:Addgene_16576 Copy
Species: Synthetic
Genetic Insert: 4xLexAOp-A2
Vector Backbone Description: Backbone Size:2327; Vector Backbone:pBSK-A2; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Enhancer
Proper citation: RRID:Addgene_165815 Copy
Species: Escherichia coli
Genetic Insert: Tus
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5240; Vector Backbone:pET24b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34161082
Comments: The plasmid backbone is originally pET24b-APEX2 (Plasmid #111702) acquired from Addgene. In this submission, the Apex2 insert has been replaced with NusA-Tus.
Proper citation: RRID:Addgene_165959 Copy
Vector Backbone Description: Backbone Size:1925; Vector Backbone:pR6Kg; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha2
Proper citation: RRID:Addgene_165863 Copy
Species: Synthetic
Genetic Insert: Vector - NT Tagging
Vector Backbone Description: Backbone Size:2287; Vector Backbone:pColE1_Alpha1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha1
Proper citation: RRID:Addgene_165868 Copy
Species: Synthetic
Genetic Insert: Basic Selection Vector
Vector Backbone Description: Backbone Size:2327; Vector Backbone:pColE1_Alpha2; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha2
Proper citation: RRID:Addgene_165882 Copy
Species: Synthetic
Genetic Insert: Eye Marker Cassette
Vector Backbone Description: Backbone Size:2287; Vector Backbone:pColE1_Alpha1; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34525356
Comments: Alpha1
Proper citation: RRID:Addgene_165872 Copy
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