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Species: Mus musculus
Genetic Insert: Srcap
Vector Backbone Description: Vector Backbone:pBSk; Vector Types:Donor plasmid; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_127903 Copy
Species: Mus musculus
Genetic Insert: Nos1
Vector Backbone Description: Backbone Size:4641; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127869 Copy
Species: Mus musculus
Genetic Insert: Nos1
Vector Backbone Description: Backbone Size:5349; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26861996
Proper citation: RRID:Addgene_127868 Copy
Species: Synthetic
Genetic Insert: Synthetic construct isolate AAV-PHP.N VP1 gene
Vector Backbone Description: Backbone Size:6314; Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32313222
Comments: Please note that this is a non-standard rep-cap construct. It uses a tTA-TRE amplification loop to increase Capsid expression and AAV production. We find that this system increases AAV titers 1.5-5 fold (Ben Deverman, Bryan Simpson and Paul Patterson, unpublished data). This is a tet-off system, so no dox or tet is needed to turn it on. It can be used like any other rep-cap plasmid. This system should only present a problem if the rAAV genome to be packaged has a tet responsive element that directs expression of a protein that affected the health of the production cells. The introduction of an XbaI restriction site for ease of cloning introduces a K449R mutation, which does not have an overt effect on vector production or transduction.
Proper citation: RRID:Addgene_127851 Copy
Species: C. griseus
Genetic Insert: IPNN-MCAK
Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22492725
Proper citation: RRID:Addgene_127856 Copy
Species: E. coli
Genetic Insert: bla
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31604953
Comments: This plasmid is a derivative of repressilator plasmids presented in: Potvin-Trottier et al, 2016, Nature.
Proper citation: RRID:Addgene_127855 Copy
Species: Klebsiella pneumoniae
Genetic Insert: CitA
Vector Backbone Description: Vector Backbone:pRSET; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22164251
Proper citation: RRID:Addgene_128020 Copy
Species: Drosophila melanogaster
Genetic Insert: LN-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:LamdaN entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128012 Copy
Genetic Insert: traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
Vector Backbone Description: Vector Backbone:pBS; Vector Types:Drosophila OSC reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128010 Copy
Species: Synthetic
Genetic Insert: PDZ-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127961 Copy
Species: Drosophila melanogaster
Genetic Insert: Gal4-DBD-3xFlag
Vector Backbone Description: Vector Backbone:modified from pAcM; Vector Types:Gal4 entry vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31384064
Proper citation: RRID:Addgene_128014 Copy
Species: Synthetic
Genetic Insert: AAVS1-CAG-KRAB-dCas9-BFP-KRAB
Vector Backbone Description: Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127966 Copy
Species: Klebsiella pneumoniae
Genetic Insert: CitA
Vector Backbone Description: Vector Backbone:pRSET; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22164251
Comments: Addgene identified the following mutation: K92A
It is not expected to impact plasmid function
Proper citation: RRID:Addgene_128019 Copy
Species: Synthetic
Genetic Insert: Myr-mCherry-3xSAG-Dr-hel-4- TrkA
Vector Backbone Description: Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30850602
Proper citation: RRID:Addgene_127963 Copy
Species: Synthetic
Genetic Insert: CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
Vector Backbone Description: Vector Backbone:pC13N; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127969 Copy
Species: Synthetic
Genetic Insert: AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
Vector Backbone Description: Vector Backbone:pAAVS1; Vector Types:Mammalian Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31422865
Proper citation: RRID:Addgene_127967 Copy
Species: Synthetic
Genetic Insert: sYFP2-OP18-mScI-R125I
Vector Backbone Description: Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128002 Copy
Genetic Insert: Pfis-cfp
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31312728
Proper citation: RRID:Addgene_127950 Copy
Species: Synthetic
Genetic Insert: Myr-3xSAG-Dr-hel4-TrkB
Vector Backbone Description: Vector Backbone:pCMVd2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30850602
Comments: Please note that there are some discrepancies between Addgene's NGS sequence and the depositor's provided partial sequence. The depositor noted that these discrepancies do not affect plasmid function.
Proper citation: RRID:Addgene_127955 Copy
Species: Synthetic
Genetic Insert: ROPY-sYFP2(Y67G)
Vector Backbone Description: Vector Backbone:pTriEx; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32265472
Proper citation: RRID:Addgene_128008 Copy
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