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Species: Synthetic
Genetic Insert: SpyCatcher002 EQ
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:6422; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29024296
Proper citation: RRID:Addgene_102830 Copy
Species: Synthetic
Genetic Insert: SpyTag002-MBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29024296
Proper citation: RRID:Addgene_102831 Copy
Species: Homo sapiens
Genetic Insert: Tmem192
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29074583
Proper citation: RRID:Addgene_102929 Copy
Vector Backbone Description: Vector Backbone:pCAP05; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28874475
Comments: pCAP05 can be linearized through the restriction sites ClaI and XhoI. Linear vector can be used for the construction of cluster-specific capture vectors using the same methodology as described for pCAP03.
Proper citation: RRID:Addgene_102920 Copy
Species: Other
Genetic Insert: DDdCas9VP192-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-tight; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29980666
Proper citation: RRID:Addgene_102889 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc14/mCherry-AID(71-114)-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27989441
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc14 in yeast.
A characterization guide can be found here:
https://www.nature.com/articles/s41598-017-04791-6
Detailed description of plasmid construction can be found in the Supplemental Experimental Procedures of the original paper:
http://www.cell.com/molecular-cell/pdfExtended/S1097-2765(16)30726-2
Proper citation: RRID:Addgene_102884 Copy
Species: Other
Genetic Insert: DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
Vector Backbone Description: Vector Backbone:PB-CAG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29980666
Proper citation: RRID:Addgene_102886 Copy
Species: Other
Genetic Insert: mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
Vector Backbone Description: Vector Backbone:pSAM; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23990803
Comments: The Plac promoter from pGFP-Mut3.1 (Clonetech), along with its associated ribosome binding sequence, was amplified via PCR. Engineered 59 BamHI and 39 NdeI restriction sites were used to sub-clone the resulting fragment into a BamHI/NdeI (New England Biolabs) double digested pSAM_Bt vector upstream of the himar1C9 transposase gene. The kanamycin resistance gene from pKD4 was amplified and ligated using the restriction sites MfeI and XbaI, replacing the erythromycin resistance gene ermG in pSAM_Bt. The resulting transposon mutagenesis vector, pSAM-Ec, was stored and propagated in the pir+ E. coli strain EcS17.
Proper citation: RRID:Addgene_102939 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:2890; Vector Backbone:pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31242442
Proper citation: RRID:Addgene_102936 Copy
Species: Other
Genetic Insert: OCT4-gRNAs-1-5-PGK-puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29980666
Proper citation: RRID:Addgene_102893 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29074583
Comments: LAMP1-RFP was cloned from Plasmid #34611
Proper citation: RRID:Addgene_102931 Copy
Species: Homo sapiens
Genetic Insert: EWS-FLI1
Vector Backbone Description: Backbone Size:7384; Vector Backbone:pCDH-puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27259270
Comments: The cDNA sequence of EWS-FLI1 was subcloned into the NheI and NotI sites of the lentiviral vector pCDH-CMV-MCS-EF1-Puro.
Proper citation: RRID:Addgene_102813 Copy
Species: Homo sapiens
Genetic Insert: Tmem192
Vector Backbone Description: Vector Backbone:pLJC5; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29074583
Proper citation: RRID:Addgene_102930 Copy
Species: Homo sapiens
Genetic Insert: EEA-5g-OSK2M2L1-gRNAs-PGK-Puro
Vector Backbone Description: Vector Backbone:PB-GG-PGK-puro; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29980666
Proper citation: RRID:Addgene_102909 Copy
Species: Other
Genetic Insert: OSK2M2L1-gRNAs-PGK-puro
Vector Backbone Description: Vector Backbone:GG-EBNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29980666
Proper citation: RRID:Addgene_102902 Copy
Species: Mus musculus
Genetic Insert: Frizzled 5
Vector Backbone Description: Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:10065; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28885975
Proper citation: RRID:Addgene_102867 Copy
Species: Mus musculus
Genetic Insert: Frizzled 1
Vector Backbone Description: Backbone Marker:Addgene #41392 (provided by David Root); Backbone Size:10065; Vector Backbone:pLEX_307; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28885975
Proper citation: RRID:Addgene_102864 Copy
Species: Synthetic
Genetic Insert: ABE7.9
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29160308
Proper citation: RRID:Addgene_102918 Copy
Species: Synthetic
Genetic Insert: ABE7.10
Vector Backbone Description: Backbone Marker:Liu lab; Backbone Size:3388; Vector Backbone:pBR322/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29160308
Proper citation: RRID:Addgene_102919 Copy
Genetic Insert: gRNAScaffold_hU6promoter
Vector Backbone Description: Backbone Size:3250; Vector Backbone:Human gRNA Expression Vector / PCR Template for STAgR; Vector Types:as PCR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29702666
Proper citation: RRID:Addgene_102843 Copy
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