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Species: Synthetic
Genetic Insert: R-GECO1.2
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23452507
Proper citation: RRID:Addgene_45494 Copy
Species: Homo sapiens
Genetic Insert: eIF4GI transcript variant 2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19114555
Comments: For diagnostic digest, an additional PvuI digestion is necessary to distinguish the insert from the vector because of their similar sizes.
The EIF4G1 translation contains M432V compared to NP_937884 (NM_198241.2); however the nucleotide change resulting in the amino acid change is more conserved between species, and it is located between functional domains of eIF4G1 (PABP-binding site and eIF4E-binding site), and does not affect its function as a translational factor
Proper citation: RRID:Addgene_45640 Copy
Species: Homo sapiens
Genetic Insert: HDAC4
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17873280
Proper citation: RRID:Addgene_45636 Copy
Species: Nostoc punctiforme
Genetic Insert: NpuDnaEC14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23974115
Comments: There are 2 mismatches between Addgene's quality control and depositor's reference sequence. These changes are in backbone region and should not affect function.
Proper citation: RRID:Addgene_45595 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7143; Vector Backbone:pcDNA DEST40; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15048125
Comments: Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt
This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc.
For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).
Proper citation: RRID:Addgene_45597 Copy
Species: Mus musculus
Genetic Insert: Lpl in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Lpl fragment was amplified by RT-PCR using the following primer pair:
TGCTGTGCAAAGAGAAGAGC
CGGACACAAAGTTAGCACCA
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 3249-3900 of NM_008509.2, not bp# 3169-3826 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45630 Copy
Species: Mus musculus
Genetic Insert: Nectin-3 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Nectin-3 fragment was amplified by RT-PCR using the following primer pair:
AAACAACCTGATCCGCAAAG
CAGTGAAAACTGTAAAGCAGCTC
For in vitro transcription, use the BamHI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 1626-2039 of NM_021495, not bp# 1624-2036 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45633 Copy
Species: Mus musculus
Genetic Insert: Ptn in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Ptn fragment was amplified by RT-PCR using the following primer pair:
GCCTACCCGTCCAAATATCC
GCCAGTTCTGGTCTTCAAGG
For in vitro transcription, use the NotI restriction digest and SP6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 1241-1830 of NM_008973, not bp# 1238-1827 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45632 Copy
Species: Rattus norvegicus
Genetic Insert: AT1AR-TSTS/A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15355986
Comments: Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. The primer for the TSTS/A receptor was:
5' p-CTCAAGCCTGTCTGCGAAAATGGCCGCTTGCTTACCGGCCTTC 3'.
N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding.
Proper citation: RRID:Addgene_45634 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Cre/Lox, DT40 cell line targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23892402
Proper citation: RRID:Addgene_45590 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Cre/Lox, DT40 cell line targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23892402
Proper citation: RRID:Addgene_45591 Copy
Species: Mus musculus
Genetic Insert: Gpr88 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Gpr88 fragment was amplified by RT-PCR using the following primer pair:
CAAATGAAACCAATGGTCAGG
TATCTGTTTCCCGTGTCTCC
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 2742-3255 of AB042408.1 or bp# 2772-3285 of NM_022427, not bp# 2742-3255 of NM_022427 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45628 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Cre/Lox, DT40 cell line targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23892402
Proper citation: RRID:Addgene_45585 Copy
Species: Mus musculus
Genetic Insert: Foxp2 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16157277
Comments: The Fox2p fragment was amplified by RT-PCR using the following primer pair:
CAGTGTGGACTGTGGACGAA
TTCCAGGTCCTCAGATAAAGG
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp#1709-2142 of AF339106, not bp# 1707-2142 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45620 Copy
Species: Nostoc punctiforme
Genetic Insert: NpR5600
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5293; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20813918
Proper citation: RRID:Addgene_45741 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Cre/Lox, DT40 cell line targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23892402
Proper citation: RRID:Addgene_45586 Copy
Species: Nostoc punctiforme
Genetic Insert: NpR5599
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5238; Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20813918
Proper citation: RRID:Addgene_45740 Copy
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET28; Vector Types:Cre/Lox, DT40 cell line targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23892402
Proper citation: RRID:Addgene_45589 Copy
Species: Mus musculus
Genetic Insert: Tcrb-V13 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16157277
Comments: The Tcrb-V13 fragment was amplified by RT-PCR using the following primer pair:
CTGAGCTGGTGGGTGAATG
AAGGTGTCAACGAGGAAGGA
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 533-1058 of X67128, not bp# 532-1059 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45622 Copy
Species: Anabaena variabilis
Genetic Insert: Ava3855
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5293; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20813918
Proper citation: RRID:Addgene_45743 Copy
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