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Species: Homo sapiens
Genetic Insert: UbcH5b
Vector Backbone Description: Backbone Marker:see PMID 1852137; Backbone Size:5000; Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11477 Copy
Species: Homo sapiens
Genetic Insert: mdm2
Vector Backbone Description: Backbone Marker:GE-Amersham; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10722742
Proper citation: RRID:Addgene_11492 Copy
Species: Homo sapiens
Genetic Insert: Frataxin
Vector Backbone Description: Backbone Marker:pBABE-hygro is available at Addgene; Backbone Size:5558; Vector Backbone:pBABE hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035806
Comments: This plasmid was cloned before there was any consensus sequence for frataxin. There are 2 mutations with the currently available frataxin sequence - L140P R165C. The effect of these mutations is unknown.
Proper citation: RRID:Addgene_11504 Copy
Species: Homo sapiens
Genetic Insert: Frataxin
Vector Backbone Description: Backbone Marker:pBABE-neo is available at Addgene; Backbone Size:5330; Vector Backbone:pBABE neo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035806
Comments: This plasmid was cloned before there was any consensus sequence for frataxin. There are 2 mutations with the currently available frataxin sequence - L140P R165C. The effect of these mutations is unknown.
Proper citation: RRID:Addgene_11503 Copy
Species: Homo sapiens
Genetic Insert: mdm2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5400; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10722742
Proper citation: RRID:Addgene_11496 Copy
Species: Homo sapiens
Genetic Insert: mdm2
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5400; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10722742
Proper citation: RRID:Addgene_11497 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 46Q glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11515 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 25 glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11508 Copy
Species: Homo sapiens
Genetic Insert: Safe Harbor Homology Sequences
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:4944; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115149 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PARK7C53A
Vector Backbone Description: Backbone Marker:Jason Moffat, Addgene plasmid # 24588; Backbone Size:7737; Vector Backbone:pLD-Cc-puro-VA; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115189 Copy
Species: Homo sapiens
Genetic Insert: gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
Vector Backbone Description: Vector Backbone:pEG302; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Insert is the same as what appears in Plasmid #106435. The plasmid backbone was changed to increase transformation efficiency.
Proper citation: RRID:Addgene_115344 Copy
Species: Homo sapiens
Genetic Insert: PARK7C53A
Vector Backbone Description: Backbone Marker:Jason Moffat, Addgene plasmid # 24588; Backbone Size:7737; Vector Backbone:pLD-Cc-puro-VA; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115184 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PARK7WT
Vector Backbone Description: Backbone Marker:Jason Moffat, Addgene plasmid # 24588; Backbone Size:7737; Vector Backbone:pLD-Cc-puro-VA; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115187 Copy
Species: Homo sapiens
Genetic Insert: CR (CRISPR/Cas9-resistant)-PARK7E163K
Vector Backbone Description: Backbone Marker:Jason Moffat, Addgene plasmid # 24588; Backbone Size:7737; Vector Backbone:pLD-Cc-puro-VA; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115191 Copy
Species: Homo sapiens
Genetic Insert: PDHA2WT
Vector Backbone Description: Backbone Marker:David Root, Addgene plasmid # 41392; Backbone Size:8270; Vector Backbone:pLEX-307; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31536960
Proper citation: RRID:Addgene_115192 Copy
Species: Homo sapiens
Genetic Insert: CHMP2B
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLNCX2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29972351
Proper citation: RRID:Addgene_115331 Copy
Species: Homo sapiens
Genetic Insert: CHMP2A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLNCX2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29972351
Proper citation: RRID:Addgene_115332 Copy
Species: Homo sapiens
Genetic Insert: PARK7C53A
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115179 Copy
Species: Homo sapiens
Genetic Insert: prostate-specific membrane antigen
Vector Backbone Description: Backbone Marker:BD Biosciences; Backbone Size:9761; Vector Backbone:pAcGP67A; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15837926
Proper citation: RRID:Addgene_11524 Copy
Species: Homo sapiens
Genetic Insert: CHMP2B
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pLNCX2; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29972351
Proper citation: RRID:Addgene_115333 Copy
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