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Species: Arabidopsis thaliana
Genetic Insert: 4-coumaroyl CoA ligase 1
Vector Backbone Description: Backbone Size:4449; Vector Backbone:pAC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16551366
Comments: Initial gene cloning described in the following:
"Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli" (2004)Kevin T. Watts, Pyung Cheon Lee Dr., Claudia Schmidt-Dannert Prof. ChemBioChem. DOI: 10.1002/cbic.200300783
Proper citation: RRID:Addgene_35947 Copy
Species: Arachis hypogaea
Genetic Insert: Stilbene synthase
Vector Backbone Description: Backbone Size:2428; Vector Backbone:pUCmod; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16551366
Proper citation: RRID:Addgene_35949 Copy
Species: Homo sapiens
Genetic Insert: Wnt7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35933 Copy
Species: Homo sapiens
Genetic Insert: Wnt11
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35922 Copy
Species: Homo sapiens
Genetic Insert: Wnt10B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35921 Copy
Species: Homo sapiens
Genetic Insert: Wnt16
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35923 Copy
Species: Homo sapiens
Genetic Insert: Wnt5A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35874 Copy
Species: Homo sapiens
Genetic Insert: Wnt5B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35875 Copy
Species: Homo sapiens
Genetic Insert: Wnt7A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35914 Copy
Species: Homo sapiens
Genetic Insert: Wnt6
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7711; Vector Backbone:pcDNA3.2/V5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22784633
Proper citation: RRID:Addgene_35913 Copy
Species: Mus musculus
Genetic Insert: zfp423 shRNA
Vector Backbone Description: Backbone Size:6700; Vector Backbone:pMKO.1; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20200519
Comments: Forward Oligo 5'
CCGGCCCTGAATGTAACGTGAAGTTCTCGAGAACTTCACGTTACATTCAGGGTTTTTG 3'
Reverse Oligo 5'
AATTCAAAAACCCTGAATGTAACGTGAAGTTCTCGAGAACTTCACGTTACATTCAGGG 3'
Proper citation: RRID:Addgene_35972 Copy
Species: Homo sapiens
Genetic Insert: heterogeneous nuclear ribonucleoprotein U
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25613133
Proper citation: RRID:Addgene_35974 Copy
Vector Backbone Description: Backbone Marker:Naldini Lab; Backbone Size:6782; Vector Backbone:pRRLsinPPT-CMV-MCS-WPRE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23792206
Comments: The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
Proper citation: RRID:Addgene_36097 Copy
Species: Homo sapiens
Genetic Insert: FYVE(EEA1) domain
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9702203
Proper citation: RRID:Addgene_36096 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 2xPH(Osh2) fusion
Vector Backbone Description: Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21295699
Comments: Please note that the first Osh2 domain contains aa273-421 and the second Osh2 domains contains aa260-421 when compared to GenBank reference sequence NP_010265.1. According to this reference sequence the PH domain is aa 292–384. The additional residues surrounding the PH domain were intentionally cloned into this construct.
Proper citation: RRID:Addgene_36095 Copy
Vector Backbone Description: Backbone Size:5450; Vector Backbone:pEBB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20671728
Comments: After mammal transfection, supplement fresh media with 4 uM Biotin to "charge" tag by Biotin. Allow cells to grow for about 48 h before the purification steps.
Proper citation: RRID:Addgene_36099 Copy
Species: Synthetic
Genetic Insert: pEBFP2-Nuc
Vector Backbone Description: Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
Proper citation: RRID:Addgene_36085 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
Proper citation: RRID:Addgene_36084 Copy
Species: Homo sapiens
Genetic Insert: cyclophilin B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7767; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22426501
Comments: Please note that Addgene's sequencing results identified a A212S mutation when compared to GenBank reference NP_000933.1
Proper citation: RRID:Addgene_36123 Copy
Species: Mus musculus
Genetic Insert: G protein beta subunit B4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17581822
Proper citation: RRID:Addgene_36114 Copy
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