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Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 NSP14
Vector Backbone Description: Backbone Size:3900; Vector Backbone:pRSF-His6-sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39663451
Proper citation: RRID:Addgene_213524 Copy
Species: SARS-CoV-2
Genetic Insert: nucleocapsid protein-T2A-membrane protein-IRES-envelope protein
Vector Backbone Description: Vector Backbone:pCSC-G; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40295734
Proper citation: RRID:Addgene_231904 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N protein (C terminal domain)
Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_228641 Copy
Species: SARS-CoV-2
Genetic Insert: 3CLpro
Vector Backbone Description: Vector Backbone:FuGW; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40780218
Proper citation: RRID:Addgene_240455 Copy
Species: SARS-CoV-2
Genetic Insert: Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Glu substitution and an N-terminal GST-tag
Vector Backbone Description: Vector Backbone:pGEX5X3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38102143
Proper citation: RRID:Addgene_218458 Copy
Species: SARS-CoV-2
Genetic Insert: Cytosolic tail of SARS-CoV-2 spike carrying Lys1269 and His1271 substitutions and an N-terminal GST-tag
Vector Backbone Description: Vector Backbone:pGEX5X3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38102143
Proper citation: RRID:Addgene_218459 Copy
Species: SARS-CoV-2
Genetic Insert: Cytosolic tail of SARS-CoV-2 spike carrying His1271Lys substitution and an N-terminal GST-tag
Vector Backbone Description: Vector Backbone:pGEX5X3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38102143
Proper citation: RRID:Addgene_218456 Copy
Species: SARS-CoV-2
Genetic Insert: Spike (KP.3.1.1)
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Preprint: https://www.biorxiv.org/content/10.1101/2022.03.04.479488v1
Proper citation: RRID:Addgene_233342 Copy
Species: SARS-CoV-2
Genetic Insert: NSP14
Vector Backbone Description: Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34862481
Proper citation: RRID:Addgene_236423 Copy
Species: SARS-CoV-2
Genetic Insert: NSP10
Vector Backbone Description: Vector Backbone:pET-30a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34862481
Proper citation: RRID:Addgene_236426 Copy
Species: SARS-CoV-2
Genetic Insert: CoV-2 frC
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37988285
Comments: detailed explanation of CLEVER reverse genetics methodology in DOI: 10.7554/eLife.89035
Proper citation: RRID:Addgene_253184 Copy
Species: SARS-CoV-2
Genetic Insert: 3CLpro
Vector Backbone Description: Backbone Marker:Novagen/Northeast Structural Genomics Consortium; Backbone Size:5737; Vector Backbone:pET15Sumo2_NESG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37235475
Comments: This construct was produced by total gene synthesis by Genscript. The DNA insert is optimized for expression in Escherichia coli.
Proper citation: RRID:Addgene_190062 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-COV-2 Mpro-C145A
Vector Backbone Description: Backbone Marker:Novagen/Northeast Structural Genomics Consortium; Backbone Size:5737; Vector Backbone:pET15Sumo2_NESG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37235475
Proper citation: RRID:Addgene_192349 Copy
Species: Sars-CoV-2
Genetic Insert: N_R203K+G204R
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194809 Copy
Species: Sars-CoV-2
Genetic Insert: E_P71L
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194804 Copy
Species: Sars-CoV-2
Genetic Insert: N_D63G
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194807 Copy
Species: Sars-CoV-2
Genetic Insert: S_Q52R
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194847 Copy
Species: Sars-CoV-2
Genetic Insert: S_L18F
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194840 Copy
Species: Sars-CoV-2
Genetic Insert: S_S982A
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194849 Copy
Species: Sars-CoV-2
Genetic Insert: S_Y144del
Vector Backbone Description: Vector Backbone:MAC-tag-C (#108077); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.21203/rs.3.rs-4322477/v1 for preprint.
Proper citation: RRID:Addgene_194854 Copy
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