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Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11165; Vector Backbone:pGWB501; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141286 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:10700; Vector Backbone:pGWB601; Vector Types:Plant Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:31316580
Comments: Plasmids pNL1.1 and pNL1.2 with NanoLUC sequence were kindly provided by Promega Corporation (UK, Delta House, Southampton Science Park, Southampton, SO16 7NS) and propagated in E. coli DH5alpha (Invitrogen, ThermoFisher Scientific, Waltham, Massachusetts, US). The NanoLUC sequence was amplified, and cloned via a pET28a(+) (Novagen, Merck, Darmstadt, Germany) intermediate vector into a pUC19 vector carrying a 3× FLAG 10× His peptide as a C-terminal translational fusion, to form NanoLUC-3× FLAG-10× His (NL3F10H). The 3× FLAG-10His was chemically synthesised by Eurogentec (Seraing, Belgium).
Proper citation: RRID:Addgene_141287 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB402; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141299 Copy
Vector Backbone Description: Backbone Marker:Tsuyoshi Nakagawa; Backbone Size:11000; Vector Backbone:pGWB401; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:32582876
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.02.10.939363v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_141296 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79441 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79446 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79449 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79445 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79444 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79451 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79454 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79453 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79457 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJOG292; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_79459 Copy
Genetic Insert: cat
Vector Backbone Description: Backbone Marker:BioBrick; Backbone Size:2406; Vector Backbone:pSB4X5; Vector Types:; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:26932362
Proper citation: RRID:Addgene_79824 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_80579 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80582 Copy
Species: E. coli
Genetic Insert: pnos:nptII-tmos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Proper citation: RRID:Addgene_80580 Copy
Species: E. coli
Genetic Insert: pnos:PAT-tnos
Vector Backbone Description: Backbone Marker:6800; Vector Backbone:pVM_BGW-BpiI; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:27579989
Comments: Please note- Functional data on this plasmid can be found in the following publication: Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations. Streubel et al PLoS One. 2017 Mar 16;12(3):e0173580. PMID: 28301511
Proper citation: RRID:Addgene_80583 Copy
Vector Backbone Description: Vector Backbone:pICH41308; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Spectinomycin
Defining Citation: PMID:29847550
Comments: The ccdB has an H55Q mutation that does not affect function.
Proper citation: RRID:Addgene_105338 Copy
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