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Species: S. elongatus PCC7942
Genetic Insert: RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
Vector Backbone Description: Backbone Size:4400; Vector Backbone:pBR322; Vector Types:Cloning vector; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101831 Copy
Species: BxbI phage
Genetic Insert: BxbI phage integrase
Vector Backbone Description: Backbone Size:3740; Vector Backbone:pSEVA6213; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227669 Copy
Species: Synthetic
Genetic Insert: msfGFP
Vector Backbone Description: Backbone Size:4546; Vector Backbone:pBAMD1-6; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:39778677
Proper citation: RRID:Addgene_227665 Copy
Vector Backbone Description: Backbone Marker:Ichiro Matsumura, Addgene plasmid #26702; Backbone Size:2515; Vector Backbone:pBAV1K-T5-gfp; Vector Types:Unspecified; Bacterial Resistance:Gentamicin
Comments: Use Gentamicin 15 µg/mL in E. coli, Gentamicin 30 µg/mL in Synechococcus sp. PCC 7002
Grow at 30°C in E. coli, grow at 37°C in Synechococcus sp. PCC 7002
For increased transformation into PCC 7002, miniprep this plasmid from E.coli strain DHIP1.
DHIP1 deposited under the following paper:
Kamoku C, Nielsen DR. Highly Iterated Palindrome 1 (HIP1) sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Published online September 10, 2024:2024.09.10.611283. doi:10.1101/2024.09.10.611283
Please visit https://doi.org/10.1101/2024.07.31.606084 for bioRxiv preprint.
Proper citation: RRID:Addgene_226658 Copy
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229955 Copy
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229954 Copy
Species: Homo sapiens
Genetic Insert: ActBT2ATetP2AP - H
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229972 Copy
Species: Homo sapiens
Genetic Insert: ActBT2ATetP2AP - mAGH
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229973 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V1 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205291 Copy
Genetic Insert: TevSpCas9, sgRNA cassette, oriT, CenArsHis
Vector Backbone Description: Backbone Size:52707; Vector Backbone:pTA-Mob; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:31586051
Proper citation: RRID:Addgene_202796 Copy
Species: Homo sapiens
Genetic Insert: SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV SpCas9; Vector Types:Mammalian Expression, CRISPR, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206267 Copy
Species: Other
Genetic Insert: H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production, CRE Acceptor (MultiBac); Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Proper citation: RRID:Addgene_206266 Copy
Species: Homo sapiens
Genetic Insert: H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
Vector Backbone Description: Vector Backbone:pMMACE DEST CMV H2B IRFP713; Vector Types:Mammalian Expression, Recombinant baculovirus production; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35801912
Comments: L224Q and A247S mutations in mKO2-hCdt1 will not affect plasmid function.
Proper citation: RRID:Addgene_206280 Copy
Species: Pseudomonas putida DOT-T1E
Genetic Insert: single-stranded DNA recombineering
Vector Backbone Description: Backbone Marker:SEVA collection; Backbone Size:5089; Vector Backbone:pSEVA624; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_199241 Copy
Species: Synthetic
Genetic Insert: CspRecT / Bxb-1
Vector Backbone Description: Vector Backbone:pKM461; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: Replicating ORBIT V2 helper plasmid w/ inducible oligo recombineering module (m-tol) and bxb-1 integrase (arabinose). SacB is present for counterselection and gentamicin is the resistance marker. V2 plasmid does not have mutL, which lowers background mutation rate. Please visit https://doi.org/10.1101/2023.06.28.546561 for bioRxiv preprint.
Proper citation: RRID:Addgene_205293 Copy
Vector Backbone Description: Backbone Size:57000; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology, Diatom Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188609 Copy
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pTA-Mob 2.0; Vector Types:Bacterial Expression, Yeast Expression, Synthetic Biology; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37850145
Proper citation: RRID:Addgene_188610 Copy
Species: Homo sapiens
Genetic Insert: XRCC6
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192302 Copy
Species: Homo sapiens
Genetic Insert: KPNB1
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2-3xFLAG; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192301 Copy
Species: Homo sapiens
Genetic Insert: PFN2_variant2
Vector Backbone Description: Backbone Marker:Addgene #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:37055441
Proper citation: RRID:Addgene_192300 Copy
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