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Species: Aequorea
Genetic Insert: unstable gfp-mut3.1 LAA
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16207908
Comments: Use gentamicin at 10 ug/mL.
Proper citation: RRID:Addgene_14463 Copy
Species: Discoma
Genetic Insert: mRFP1
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pbbr; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:16207908
Comments: Use gentamicin at 10 ug/mL.
Proper citation: RRID:Addgene_14471 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (Microtubule Associated Protein 7)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159718 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (only amino acids 1-560), K560
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159720 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (only amino acids 1-490), K490
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159721 Copy
Species: Homo sapiens
Genetic Insert: MAP7 (only amino acids 1-316)(includes MTBD and P123 domains)
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159728 Copy
Species: Homo sapiens
Genetic Insert: KIF5B (full length, 1-963 amino acids), K963
Vector Backbone Description: Vector Backbone:pOmnibac; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:35050657
Proper citation: RRID:Addgene_159727 Copy
Vector Backbone Description: Backbone Marker:Addgene #25752; Backbone Size:4496; Vector Backbone:pEN_TTmiRc2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:29161592
Proper citation: RRID:Addgene_83274 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Size:9449; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, Broad-host range; Bacterial Resistance:Gentamicin
Defining Citation: PMID:20522779
Proper citation: RRID:Addgene_90103 Copy
Genetic Insert: YcgR
Vector Backbone Description: Backbone Size:9449; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, Broad-host range; Bacterial Resistance:Gentamicin
Defining Citation: PMID:20522779
Proper citation: RRID:Addgene_90102 Copy
Genetic Insert: YFP
Vector Backbone Description: Backbone Size:9449; Vector Backbone:pMMB67EH; Vector Types:Bacterial Expression, Broad-host range; Bacterial Resistance:Gentamicin
Defining Citation: PMID:20522779
Proper citation: RRID:Addgene_90104 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Addgene #83274; Backbone Size:4496; Vector Backbone:pEN_TTmiRC2 3xFLAG; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28174228
Proper citation: RRID:Addgene_87778 Copy
Species: Discosoma sp.
Genetic Insert: E2-Crimson
Vector Backbone Description: Vector Backbone:pUCP30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:26937640
Comments: There is an ER retention signal at the C-terminus of the E2-Crimson ORF. This sequence is based on the template used to generate this vector. It is not predicted to impact expression or function in bacteria.
Proper citation: RRID:Addgene_78478 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
Proper citation: RRID:Addgene_78501 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: Allows different restriction cloning strategies to be used than pJQ200SK. Since full length lacZ alpha is present, blue white selection works faster than for pJQ200SK
Proper citation: RRID:Addgene_78500 Copy
Vector Backbone Description: Backbone Marker:Jurgen Quandt; Backbone Size:4700; Vector Backbone:pJQ200; Vector Types:Suicide vector for gene replacement; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: The new synthetic polylinker (SmaI site flanked by NotI) allows access to restriction sites within a cloned fragment to allow insertion of cassettes etc. Blue/White selection works.
Proper citation: RRID:Addgene_78502 Copy
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pJQ200; Vector Types:Suicide plasmid; Bacterial Resistance:Gentamicin
Defining Citation: PMID:8486283
Comments: This is a suicide vector for gene replacement. It is identical to pJQ200SK except that the multiple cloning site is from pBluescriptKS instead of from pBluescriptSK
Proper citation: RRID:Addgene_78498 Copy
Species: Chlorocebus pygerythrus pygerythrus
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Comments: Note that the TRIM5 insert in this plasmid contains a V434A polymorphism compared to GenBank ID AAW72440.1. Please see the full plasmid sequence for the most accurate information.
Proper citation: RRID:Addgene_79031 Copy
Species: Homo sapiens
Genetic Insert: OSF-TRIM5alpha(human)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79034 Copy
Species: Chlorocebus pygerythrus pygerythrus
Genetic Insert: OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:27253068
Proper citation: RRID:Addgene_79030 Copy
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