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Genetic Insert: Formylglycine-generating enzyme
Vector Backbone Description: Vector Backbone:pRSF-Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29458769
Comments: FSG gene on PfoI-XhoI fragment from pBAD/myc-hisA Rv0712 (FGE) (Carolyn Bertozzi, Addgene plasmid # 16132) was cloned into pRSF-Duet PfoI-XhoI cut
FGE(rv0712) is nt 4810-5709
Proper citation: RRID:Addgene_102615 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4700; Vector Backbone:EGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102783 Copy
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102701 Copy
Genetic Insert: J23106:B0034:spisPink:rrnBT1-T7TE
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2195; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102703 Copy
Species: Homo sapiens
Genetic Insert: APPL1
Vector Backbone Description: Backbone Size:4722; Vector Backbone:mCherry-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: *Note: Addgene's quality control sequencing finds additional residues KPNSAVDGTAGPGSTGSR appended to the C-terminus of the APPL1 protein. These additional residues are not thought to affect protein function.
Proper citation: RRID:Addgene_102784 Copy
Species: Homo sapiens
Genetic Insert: Rab5a
Vector Backbone Description: Backbone Size:4731; Vector Backbone:EGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: mCherry-C2-Rab5a encodes wild-type human Rab5a,(NM_004162) as a fusion protein with mCherry. Rab5a was inserted between the Eco R1 and Sal 1 restriction sites. mCherry-C2 encodes a monomeric variant of DsRed. The backbone plasmid is pEGFP-C1 (Clontech). The C2 version of mCherry was created by insertion of a short piece of DNA between Xho 1 and EcoR1 restriction sites. In the resulting multiple cloning site, the Sac 1 restriction site has been eliminated and the Eco R1 restriction site is in frame.
Proper citation: RRID:Addgene_102785 Copy
Species: T7 phage
Genetic Insert: T7 RNA polymerase
Vector Backbone Description: Backbone Marker:Qiagen; Vector Backbone:pQE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29120463
Proper citation: RRID:Addgene_102790 Copy
Genetic Insert: Linker sequence
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102714 Copy
Genetic Insert: Linker sequence
Vector Backbone Description: Backbone Marker:Self-made; Backbone Size:2267; Vector Backbone:derived from pSB1K3; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29293531
Proper citation: RRID:Addgene_102710 Copy
Species: Homo sapiens
Genetic Insert: EFNA1
Vector Backbone Description: Vector Backbone:pAd Easy1; Vector Types:Adenoviral; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17079451
Comments: This construct is a adenoviral vector which carries human EFNA1 cDNA and ready to produce adenovirus which express Ephrin-A1 by tranfection into 293A cells followed by PacI digestion.
Proper citation: RRID:Addgene_102724 Copy
Species: Synthetic
Genetic Insert: SpyTag002-MBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29024296
Proper citation: RRID:Addgene_102831 Copy
Species: Homo sapiens
Genetic Insert: Human platelet membrane protein GPIb alpha 1-290aa
Vector Backbone Description: Backbone Size:5374; Vector Backbone:ET8; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28831047
Proper citation: RRID:Addgene_102878 Copy
Genetic Insert: gRNAScaffold_hU6promoter
Vector Backbone Description: Backbone Size:3250; Vector Backbone:Human gRNA Expression Vector / PCR Template for STAgR; Vector Types:as PCR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29702666
Proper citation: RRID:Addgene_102843 Copy
Genetic Insert: STAgR Insert gRNAScaffold_mU6
Vector Backbone Description: Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29702666
Proper citation: RRID:Addgene_102844 Copy
Genetic Insert: STAgR Insert SAM_mU6
Vector Backbone Description: Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29702666
Proper citation: RRID:Addgene_102845 Copy
Genetic Insert: gRNAScaffold_h7SKpromoter
Vector Backbone Description: Vector Backbone:pcDNA1; Vector Types:as PCR template; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29702666
Proper citation: RRID:Addgene_102842 Copy
Species: Mus musculus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Size:4141; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25948262
Proper citation: RRID:Addgene_102949 Copy
Species: Synthetic
Genetic Insert: TIMER-bac
Vector Backbone Description: Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25126781
Comments: TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance.
Proper citation: RRID:Addgene_103057 Copy
Species: Homo sapiens
Genetic Insert: CIBN-tagBFP-hNCL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts)
Proper citation: RRID:Addgene_103805 Copy
Species: Homo sapiens
Genetic Insert: CIBN-tagBFP-hTRF2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3932; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29122982
Comments: EGFP was cut out from the backbone (NheI/BamHI cuts)
Proper citation: RRID:Addgene_103803 Copy
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