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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Authority Record Last Update Mentions Count
aMHC-mCherry-Rex-Blasticidin
 
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1+ mentions
RRID:Addgene_21228 alpha myosin heavy chain promoter Mus musculus Ampicillin PMID:19352491 There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a "C" adjacent to "GGG". The construct should be correct. Backbone Size:7338; Vector Backbone:SMPU; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:00 5
pRosa26-DEST
 
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10+ mentions
RRID:Addgene_21189 GateWay conversion cassette Chloramphenicol and Ampicillin PMID:19014667 pRosa26-DEST was made by inserting a GateWay conversion cassette into the XhoI site of pBigT, followed by transfer of PacI / AscI fragment of the resulting vector to the pRosa26-PA vector (see http://www.biomedcentral.com/1471-213X/1/4 for details on these vectors). The resulting vector resembles the construct that was used for the R26R Cre reporter mouse made by Phil Soriano in which the expression of lacZ is coupled to the endogenous Rosa26 locus via a splice acceptor site, but only after removal of the lox-STOP-lox cassette (which is actually the neo-R cassette used during the targeting). The only thing changed from the pBigT vectors and the R26R reporter is the cloning strategy, so the resulting knock-in should be as good as these systems, and as good (or as bad) as the use of the Rosa26 locus in any situation. Stbl3 cells are advisable to use after users have done the LR recombination reaction. See http://www.hgu.mrc.ac.uk/people/n.hastie_rosa.html for more information and protocols for this plasmid. Further advice from the depositing lab: "Grow single colonies in Amp + Cam at 30 C, definitively not 37. Including the Cam should ensure that spontaneous recombinants that loose the Gateway cassette don't survive... - Do minipreps and test with the double digest PacI / AscI. This should give 4.9 kb + 9.8 kb. If this is okay, the vector should be okay" Please note that there are discrepancies between Addgene's quality control sequence and the theoretical sequence supplied by the depositing lab. These discrepancies should not affect the function of the construct. Backbone Size:14820; Vector Backbone:pRosa26-PA; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin Addgene 2026-09-26 02:29:59 18
GFP-C1-CAMKIIalpha-T286A
 
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1+ mentions
RRID:Addgene_21220 CAMKIIalpha-T286A Rattus norvegicus Kanamycin PMID:10102820 Backbone Marker:Clontech; Backbone Size:0; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin T286A (Lab plasmid KS 0003). Addgene 2026-09-26 02:30:00 1
GFP-C1-CAMKIIalpha
 
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10+ mentions
RRID:Addgene_21226 CAMKIIalpha Rattus norvegicus Kanamycin PMID:9768845 Backbone Marker:Clontech; Backbone Size:4725; Vector Backbone:C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin Lab plasmid KS 0001. Addgene 2026-09-26 02:30:00 10
PL-SIN-EOS-S(4+)-EGFP
 
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1+ mentions
RRID:Addgene_21317 Enhanced Green Fluorescence Protein Ampicillin PMID:19404254 EGFP driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6531; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 1
pMXs-mRFP1
 
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1+ mentions
RRID:Addgene_21315 monomeric Red Fluorescence Protein 1 Ampicillin PMID:19404254 mRFP1 (maximum Excitation wave length, 584 nm; maximum Emission wave length, 607 nm). pMXs MoMLV-based retroviral vector from Dr. Toshio Kitamura (Institute of Medical Science, University of Tokyo). Backbone Size:4582; Vector Backbone:pMXs MoMLV-based retroviral vector; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 5
Axin2 probe
 
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1+ mentions
RRID:Addgene_21277 Axin2 Mus musculus Ampicillin PMID:11809808 Tong DNA #94. The DNA is a HinDIII fragment of mouse Axin2 (nucleotides 1-2397) in pBluescript-KS. The antisense and sense Axin2 riboprobes were made by linearizing this plasmid with NotI or SalI and transcribing with T3 or T7 RNA polymerase, respectively. List of DNAs says " ~2 kb DIII fragment of Axin2 (1-2397) in pBS-KS”. Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:For riboprobe synthesis; Bacterial Resistance:Ampicillin cDNA bp 1774 to 2787 Addgene 2026-09-26 02:30:00 4
Axin2-luciferase
 
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1+ mentions
RRID:Addgene_21275 Axin2 promoter Mus musculus Ampicillin PMID:11809808 A 5.6-kb DNA fragment containing the Axin2 promoter, exon 1, intron 1, and part of exon 2 was subcloned from a mouse BAC clone and sequenced. In Author's map, exons are shown as gray boxes, and the sequence is numbered with bp 1 corresponding to the start of the cDNA sequence. Eight conserved Tcf/LEF binding sites (underlined and designated T1 to T8) were identified. Backbone Marker:Promega; Backbone Size:4800; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:00 7
PL-SIN-EOS-S(4+)-EiP
 
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1+ mentions
RRID:Addgene_21314 Enhanced Green Fluorescence Protein, Puromycin resistance gene Ampicillin PMID:19404254 EGFP and Puro resistance genes driven by EOS-S(4+) promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6626; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 5
Myc-Axin2
 
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1+ mentions
RRID:Addgene_21279 Axin2 Mus musculus Ampicillin PMID:11809808 Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:00 2
pcDNA-FIH1
 
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1+ mentions
RRID:Addgene_21399 HIF1AN Homo sapiens Ampicillin PMID:12615973 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 5
PL-SIN-Nanog-EGFP
 
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1+ mentions
RRID:Addgene_21321 Enhanced Green Fluorescence Protein Ampicillin PMID:19404254 EGFP driven by Nanog promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6531; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 7
pCS2-MT mouse Axin (Axin MTFu1)
 
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1+ mentions
RRID:Addgene_21287 Axin Mus musculus Ampicillin PMID:9230313 Backbone Size:4300; Vector Backbone:pCS2+MT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:00 3
PL-SIN-EF1α-EGFP
 
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10+ mentions
RRID:Addgene_21320 Enhanced Green Fluorescence Protein Ampicillin PMID:19404254 EGFP driven by ubiquitous EF1α promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G). Backbone Marker:Buzina et al., PLoS Genetics, 2008; Backbone Size:6881; Vector Backbone:PL-SIN lentiviral vector; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 13
TRX1 His C32S C35S
 
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1+ mentions
RRID:Addgene_21286 TRX1 Homo sapiens Ampicillin PMID:12089063 Backbone Size:5400; Vector Backbone:pcDNA3-His; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C32S C35S Addgene 2026-09-26 02:30:00 2
TRX1 flag C35S
 
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1+ mentions
RRID:Addgene_21285 TRX1 Homo sapiens Ampicillin PMID:12089063 Backbone Size:5400; Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C35S Addgene 2026-09-26 02:30:00 4
pBSKS Notch4
 
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1+ mentions
RRID:Addgene_21325 Notch4 Mus musculus Ampicillin PMID:8681805 Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBSKS; Vector Types:Cloning Vector; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 1
pHR'-ROMK1-CITE-GFP
 
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1+ mentions
RRID:Addgene_21322 ROMK1 Rattus norvegicus Ampicillin PMID:10938328 Backbone Marker:Didier Trono; Backbone Size:10000; Vector Backbone:pHR'; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 1
TRX1 flag C32S
 
Resource Report
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1+ mentions
RRID:Addgene_21284 TRX1 Homo sapiens Ampicillin PMID:12089063 Backbone Size:5400; Vector Backbone:pcDNA3-FLAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin C32S Addgene 2026-09-26 02:30:00 3
pHIV-Luciferase
 
Resource Report
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10+ mentions
RRID:Addgene_21375 Ampicillin The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published. NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function. Backbone Size:8713; Vector Backbone:pSICO; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin Addgene 2026-09-26 02:30:01 32

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