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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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morc3b_R (OZ576) Resource Report Resource Website |
RRID:Addgene_35216 | Zinc finger array targeting morc3b | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene morc3b. Please note that this plasmid does NOT contain the morc3b sequence. Users must order the complementary plasmid morc3b_L (OZ575) [Addgene plasmid 35215] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGGAGGA-3' in a bacterial two hybrid assay, and resulted in 10.9 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:05 | 0 | |
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zgc:162148_L (OZ579) Resource Report Resource Website |
RRID:Addgene_35219 | Zinc finger array targeting zgc:162148 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene zgc:162148. Please note that this plasmid does NOT contain the zgc:162148 sequence. Users must order the complementary plasmid zgc:162148_R (OZ580) [Addgene plasmid 35220] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290/292 (Addgene plasmids 21872 & 21873), pMLM800/802 (Addgene plasmids 27202 & 27203). The difference between pMLM800/802 and pMLM290/292 is the length of the “spacer” sequence in the full ZFN target site. If the spacer is 7 bp, scientists should use pMLM800/802. If the spacer is 5 or 6 bps, scientists should use pMLM290/292. pMLM290/292 is identical to pST1374 except that it harbors two mutations in the FokI nuclease domain (Q486E, I499L; aka the “-“ mutation see Miller et al., Nat. Biotech 2007, PMID 17603475) which confers heterodimeric behavior on these domains This zinc finger array was tested for binding activity to the sequence 5'-GCTGCTGGA-3' in a bacterial two hybrid assay, and resulted in 3.1 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082) "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934) | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:05 | 0 | |
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pSYC-201 Resource Report Resource Website |
RRID:Addgene_178963 | Glial fibrillary acidic protein promoter | Danio rerio | Ampicillin | PMID:28882119 | Backbone Size:3323; Vector Backbone:pminiTol2; Vector Types:Tol2 mediated recombination; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:20 | 0 | ||
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pSYC-204 Resource Report Resource Website |
RRID:Addgene_178964 | Glial fibrillary acidic protein promoter | Danio rerio | Ampicillin | PMID:28882119 | Backbone Size:3323; Vector Backbone:pminiTol2; Vector Types:Tol2 mediated recombination; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:22 | 0 | ||
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pAC8-TwinStrep-3C-DrHDAC6 (40-831) Resource Report Resource Website |
RRID:Addgene_180226 | Histone deacetylase 6 | Danio rerio | Ampicillin | PMID:36255643 | Vector Backbone:pAC8; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:26 | 0 | ||
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pPRISM-TagRFP-gcry-mRFP Resource Report Resource Website |
RRID:Addgene_117766 | TagRFP | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-TagRFP-cmlc2-TagBFP Resource Report Resource Website |
RRID:Addgene_117768 | TagRFP | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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Control-cx43.4-48H-pPRISM Resource Report Resource Website |
RRID:Addgene_117801 | TagRFP | Danio rerio | Kanamycin | PMID:32412410 | Control integrates at the gene cx43.4 using the guide RNA Target "5'-GGGCCATATCTTGCCCACGA-3'" for wide-spread RFP expression in zebrafish embryos from 12hrs post injection through 5 days development | Backbone Marker:Karl J. Clark; Backbone Size:2329; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Cre-cmlc2-TagBFP Resource Report Resource Website |
RRID:Addgene_117792 | Cre | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Cre-gcry-eGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_117791 | Cre | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 2 | |
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pPRISM-Cre-cmlc2-mRFP Resource Report Resource Website |
RRID:Addgene_117793 | Cre | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Fuse-gcry-mRFP Resource Report Resource Website |
RRID:Addgene_117784 | mRFP | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Fuse-cmlc2-TagBFP Resource Report Resource Website |
RRID:Addgene_117786 | mRFP | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-TagBFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-gal4-VP16-cmlc2-mRFP Resource Report Resource Website |
RRID:Addgene_117781 | gal4/VP16 | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Cre-gcry-TagBFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_117789 | Cre | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-TagBFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 1 | |
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pPRISM-Fuse-cmlc2-eGFP Resource Report Resource Website |
RRID:Addgene_117788 | mRFP | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-eGFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Cre-gcry-mRFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_117790 | Cre | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 1 | |
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pPRISM-Stop-gcry-eGFP Resource Report Resource Website |
RRID:Addgene_117773 | 3x3 Stop sequence | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-eGFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Stop-cmlc2-mRFP Resource Report Resource Website |
RRID:Addgene_117775 | 3x3 Stop sequence | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a cardiac-myosin-light-chain-2 promoter driving NLS-mRFP, Oline tools and cloning help avalible at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 0 | |
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pPRISM-Stop-gcry-mRFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_117772 | 3x3 Stop sequence | Danio rerio | Kanamycin | PMID:32412410 |
Secondary gene is a gamma-crystalin promoter driving NLS-mRFP. Online tools and cloning help available at |
Backbone Marker:Karl J. Clark; Backbone Size:2856; Vector Backbone:pK; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:57 | 1 |
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