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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDONR221-ZNF808 Resource Report Resource Website |
RRID:Addgene_101470 | ZNF808 | Homo sapiens | Kanamycin | PMID:33257861 | Harvard generated by gene synthesis | Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions | Addgene | 2026-09-26 02:18:24 | 0 |
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pDONR221-ASCL3 Resource Report Resource Website |
RRID:Addgene_101515 | ASCL3 | Homo sapiens | Kanamycin | PMID:33257861 | Harvard generated by gene synthesis | Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions | Addgene | 2026-09-26 02:18:25 | 0 |
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pDONR221-HOXB3 Resource Report Resource Website |
RRID:Addgene_101478 | HOXB3 | Homo sapiens | Kanamycin | PMID:33257861 | Harvard generated by gene synthesis | Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions | Addgene | 2026-09-26 02:18:24 | 0 |
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pDONR221-ZNF716 Resource Report Resource Website |
RRID:Addgene_101481 | ZNF716 | Homo sapiens | Kanamycin | PMID:33257861 | Harvard generated by gene synthesis | Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions | Addgene | 2026-09-26 02:18:24 | 0 |
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pDONR221-ZNF275 Resource Report Resource Website |
RRID:Addgene_101487 | ZNF275 | Homo sapiens | Kanamycin | PMID:33257861 | Harvard generated by gene synthesis | Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions | Addgene | 2026-09-26 02:18:24 | 0 |
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pMH-SFB-DYRK1A Resource Report Resource Website 1+ mentions |
RRID:Addgene_101770 | DYRK1A | Homo sapiens | Ampicillin | PMID:38966995 | Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | siRNA-resistant allele | Addgene | 2026-09-26 02:18:26 | 1 | |
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pMH-SFB-DYRK1B Resource Report Resource Website 1+ mentions |
RRID:Addgene_101771 | DYRK1B / MIRK | Homo sapiens | Ampicillin | PMID:32611815 | Vector Backbone:pMH-SFB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | siRNA-resistant allele | Addgene | 2026-09-26 02:18:26 | 1 | |
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H14-MBP-SUMO-Cys-Linker-BAF_G47E Resource Report Resource Website 1+ mentions |
RRID:Addgene_101779 | BAF (BANF1) | Homo sapiens | Kanamycin | PMID:28841419 | Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | G47E mutation | Addgene | 2026-09-26 02:18:26 | 1 | |
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EGFP-P2A_BAF_G47E Resource Report Resource Website 1+ mentions |
RRID:Addgene_101774 | BAF (BANF1) | Homo sapiens | Ampicillin | PMID:28841419 | Backbone Marker:Feng Zhang; Backbone Size:10370; Vector Backbone:Addgene plasmid # 52962; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | G47E mutation | Addgene | 2026-09-26 02:18:26 | 1 | |
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AICSDP-22:CETN2-mTagRFP-T Resource Report Resource Website 1+ mentions |
RRID:Addgene_101781 | CETN2 Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | Addgene | 2026-09-26 02:18:26 | 1 | |
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AICSDP-19:LAMP1-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101782 | LAMP1 Homology Arms with linker-mEGFP | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human LAMP1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: EFGSTGSTGSTGADPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:18:26 | 3 | ||
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AICSDP-27:SLC25A17-mEGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101784 | SLC25A17 Homology Arms with linker-mEGFP | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SLC25A17 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: RDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | Addgene | 2026-09-26 02:18:26 | 1 | |
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AICSDP-28:TUBA1B-mTagRFP-T Resource Report Resource Website 1+ mentions |
RRID:Addgene_101785 | TUBA1B Homology Arms with mTagRFPT-linker | Homo sapiens | Ampicillin | This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . | Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin | homology arms contain point mutations to disrupt crRNA binding sites used | Addgene | 2026-09-26 02:18:26 | 1 | |
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pGL3-Sox2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101761 | SRY-box 2 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:18:26 | 7 | ||
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pGL3-TGFb1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_101762 | transforming growth factor beta 1 promoter | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:18:26 | 6 | ||
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CS2 Flag-Smad2 (3A) Resource Report Resource Website 1+ mentions |
RRID:Addgene_101763 | SMAD2 | Homo sapiens | Ampicillin | PMID:29593326 | Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Three Ser to Ala mutations in the phosphorylation sites (S464A, S465A, S467A) | Addgene | 2026-09-26 02:18:26 | 1 | |
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pFastBac1_FLAG-BPTF Resource Report Resource Website |
RRID:Addgene_102242 | Flag-BPTF | Homo sapiens | Ampicillin | BPTF insert contains amino acid residues 140-2903 with C1746R, T1752A, D1852N variants compared to the NCBI reference [NM_004459.6]. | Backbone Marker:Invitrogen; Backbone Size:4775; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:18:27 | 0 | ||
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pHR-EF1a-dCas9-HA-BFP-KRAB-NLS Resource Report Resource Website 1+ mentions |
RRID:Addgene_102244 | dCas9-HA-BFP-KRAB-NLS | Homo sapiens | Ampicillin | Contains HA tag and TagBFP fusion. The plasmid is derived from Addgene Plasmid #46911. The promoter was changed to human EF1a and the NLS was moved to the C-terminus of the fusion protein. | Backbone Size:9651; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin | Addgene | 2026-09-26 02:18:27 | 7 | ||
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LAMP-mCherry-CRY2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_102249 | LAMP-mCherry-CRY2 | Homo sapiens | Kanamycin | PMID:25963241 | Backbone Size:4000; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | Addgene | 2026-09-26 02:18:27 | 3 | ||
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pTO_CHMP2B_LAP Resource Report Resource Website 1+ mentions |
RRID:Addgene_101848 | CHMP2B | Homo sapiens | Ampicillin | PMID:28604678 | Part of the plasmid was derived from pmCherry_N_dest from the Helenius Lab (ETH Zuerich) | Backbone Size:6098; Vector Backbone:pTO_LAP_N-DEST; Vector Types:Mammalian Expression, Bacterial Expression, Flp-FRT recombination, Tet-inducible expression; Bacterial Resistance:Ampicillin | silent mutation in Thr 104; A to T | Addgene | 2026-09-26 02:18:26 | 1 |
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