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Species: Mus musculus
Genetic Insert: Sp2
Vector Backbone Description: Backbone Marker:Guntram Suske (Addgene plasmid # 24544); Backbone Size:3996; Vector Backbone:pN3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The plasmid is related to the Addgene plasmids pN3-Sp1FL (#24543), pN3-Sp3FL (#24541) and pN3-Control (#24544)
Proper citation: RRID:Addgene_107718 Copy
Species: Mus musculus
Genetic Insert: Mmu-miR-30a-5p mature sequence
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5699; Vector Backbone:pcDNA6.2-GW/EmGFP; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Spectinomycin
Comments: Mmu-miR-30a-5p mature sequence was cloned following manufacturer’s instructions. It generates an artificial pre-miRNA, which matures to mmu-miR-30a-5p. Plasmid constructed by Marc Beltrà in the Penna lab.
Proper citation: RRID:Addgene_107789 Copy
Species: Mus musculus
Genetic Insert: Estrogen-related receptor gamma
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107768 Copy
Species: Mus musculus
Genetic Insert: Id1
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:8650; Vector Backbone:pCDH-EF1mcs-PGKpuro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28794185
Proper citation: RRID:Addgene_107735 Copy
Species: Mus musculus
Genetic Insert: mCD44v4
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS results identified R350G and N506S variants compared to the NCBI reference. These are polymorphisms that should not affect plasmid function according to the depositing lab.
Proper citation: RRID:Addgene_107969 Copy
Species: Mus musculus
Genetic Insert: Egr2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: A Sal I site was introduced before the HindIII site to further transfer to pBabe.
Proper citation: RRID:Addgene_107997 Copy
Species: Mus musculus
Genetic Insert: cGK1 2066bp
Vector Backbone Description: Vector Backbone:pCMV-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_107975 Copy
Species: Mus musculus
Genetic Insert: mCD44v7
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Addgene NGS results identified P22del H23del, S196G, H321Y, and G334S variants compared to the NCBI reference. These are polymorphisms that should not affect plasmid function according to the depositing lab.
Proper citation: RRID:Addgene_107971 Copy
Species: Mus musculus
Genetic Insert: Egr4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5427; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: A Sal I site was introduced before the HindIII site to further transfer to pBabe.
Proper citation: RRID:Addgene_108096 Copy
Species: Mus musculus
Genetic Insert: Egr4
Vector Backbone Description: Backbone Size:5086; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: The Egr4 gene was transferred from pcDNA3.1-Egr4 (Addgene plasmid 108096) to the pBabe(puro) retroviral vector using EcoRI and SalI sites.
Proper citation: RRID:Addgene_108105 Copy
Species: Mus musculus
Genetic Insert: Egr3
Vector Backbone Description: Backbone Size:5086; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: The Egr3 gene was transferred from pcDNA3.1-Egr3 (Addgene plasmid 107998) to the pBabe(puro) retroviral vector using EcoRI and SalI sites.
Proper citation: RRID:Addgene_108102 Copy
Species: Mus musculus
Genetic Insert: Vmn2r120
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29465786
Proper citation: RRID:Addgene_108087 Copy
Species: Mus musculus
Genetic Insert: Vmn2r118
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3015; Vector Backbone:pGEM-T Easy; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29465786
Proper citation: RRID:Addgene_108086 Copy
Species: Mus musculus
Genetic Insert: shRNA to Egr4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6445; Vector Backbone:pSirenRetroQ(puro); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: For the chosen sequence (AAGTCTTTGGATGTGATGTTT), the annealed oligos are:
5'-gatccAAGTCTTTGGATGTGATGTTTTTCAAGAGAAAACATCACATCCAAAGACTTTTTTTTACGCGTg-----3'
3'-----gTTCAGAAACCTACACTACAAAAAGTTCTCTTTTGTAGTGTAGGTTTCTGAAAAAAAATGCGCActtaa-5'
Proper citation: RRID:Addgene_108117 Copy
Species: Mus musculus
Genetic Insert: shRNA to Egr3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6445; Vector Backbone:pSirenRetroQ(puro); Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535333
Comments: For the chosen sequence (AATCAATTGCCTGACAATC), the annealed oligos were:
5'-gatccAATCAATTGCCTGACAATCTTCAAGAGAGATTGTCAGGCAATTGATTTTTTTTACGCGTg-----3'
3'-gTTAGTTAACGGACTGTTAGAAGTTCTCTCTAACAGTCCGTTAACTAAAAAAAATGCGCActtaa-5'
Proper citation: RRID:Addgene_108115 Copy
Species: Mus musculus
Genetic Insert: Vmn2r1-Hprt (5')-loxP-neo
Vector Backbone Description: Backbone Marker:Agilent Technologies/Stratagene; Backbone Size:2900; Vector Backbone:pBluescript II SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29465786
Proper citation: RRID:Addgene_108079 Copy
Species: Mus musculus
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108403 Copy
Species: Mus musculus
Genetic Insert: PKD1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108402 Copy
Species: Mus musculus
Genetic Insert: Pkd1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108395 Copy
Species: Mus musculus
Genetic Insert: Pkd1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:modified pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29426897
Proper citation: RRID:Addgene_108392 Copy
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