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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
U6-U57A
 
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RRID:Addgene_169927 U6 U57A Saccharomyces cerevisiae Ampicillin PMID:33547186 Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone. Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 0
U6-U57C
 
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RRID:Addgene_169928 U6 U57C Saccharomyces cerevisiae Ampicillin PMID:33547186 Please note that this plasmid contains an additional IS4-like element ISVsa5 family transposase compared to the pRS314 backbone. Backbone Marker:ATCC; Backbone Size:4783; Vector Backbone:pRS314; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:03 0
pET28a(+)-HisTim12_CtoA
 
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RRID:Addgene_170281 yeast Tim12 Saccharomyces cerevisiae Kanamycin PMID:33974880 Vector Backbone:pET28a+; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin mutated two non-conserved Cys to Ala 2026-08-15 01:10:06 0
pRE57I-GNAc
 
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RRID:Addgene_170637 Mini-Tn6677, GNAc cassette, lacI Saccharomyces cerevisiae Ampicillin PMID:34152213 Vector Backbone:pUC57mini; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:10 0
pEDJ8
 
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RRID:Addgene_177271 gRNA_RNH1_RNH201 Saccharomyces cerevisiae Ampicillin PMID:34107026 Vector Backbone:p0054; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
pEDJ333
 
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RRID:Addgene_177272 Cas9 Saccharomyces cerevisiae Ampicillin PMID:34107026 Backbone Size:6300; Vector Backbone:pRS415U; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
pEDJ508
 
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RRID:Addgene_177275 ADH1t:T7p:cgRNA_HIS3_stop:tZ Saccharomyces cerevisiae Ampicillin PMID:34107026 Backbone Size:4300; Vector Backbone:pEDJ400; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
pEDJ506
 
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RRID:Addgene_177274 ADH1t:HIS3_23∆29-XII-5 :<-PGK1p Saccharomyces cerevisiae Ampicillin PMID:34107026 Integrative plasmid Please note: Plasmid contains a 68bp insertion in mADH1t compared to the depositor's provided sequence. This insertion is not known to affect plasmid function. Backbone Size:4500; Vector Backbone:pCfB2909; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:06 0
Tubp-Gal80 in pCaSpeR4
 
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1+ mentions
RRID:Addgene_17748 GAL80 Saccharomyces cerevisiae Ampicillin PMID:10197526 Backbone Size:7855; Vector Backbone:pCaSpeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:11:08 8
LT1_33_pTEFmut8-mCherry
 
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RRID:Addgene_177706 pTEFmut8-mCherry Saccharomyces cerevisiae Ampicillin PMID:35069506 Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Ampicillin 2026-08-15 01:11:10 0
LT2_14_GlyOx
 
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RRID:Addgene_177711 pTEFmut8-mTurquoise2-RNA_2.6--tCYC1 Saccharomyces cerevisiae Kanamycin PMID:35069506 Vector Backbone:Empty backbone; Vector Types:Yeast Expression, Used as donor DNA in genome integration after linearisation with NotI; Bacterial Resistance:Kanamycin 2026-08-15 01:11:10 0
p2B-56
 
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RRID:Addgene_177925 Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter Saccharomyces cerevisiae Ampicillin PMID:27323850 Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information. Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin V9I; F67S; G72P, F86Y; R171K 2026-08-15 01:11:12 0
p2B-55
 
Resource Report
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1+ mentions
RRID:Addgene_177924 Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter Saccharomyces cerevisiae Ampicillin PMID:27323850 Please refer to Roney et al. 2016 Sci Rep. "Improvement of the reverse tetracycline transactivator by single amino acid substitutions that reduce leaky target gene expression to undetectable levels" (doi: 10.1038/srep27697) for more information. Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin V9I; F67S; G72P, F86Y; R171K 2026-08-15 01:11:12 1
pMBI
 
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1+ mentions
RRID:Addgene_17816 mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase Saccharomyces cerevisiae Tetracycline PMID:12778056 does not contain lacI/ ERG1, ERG8, MVD1: S. cerevisiae/ idi: E. Coli Backbone Size:0; Vector Backbone:n/a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline 2026-08-15 01:11:14 2
pBAD24MevT
 
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RRID:Addgene_17820 MevT Saccharomyces cerevisiae Ampicillin PMID:17239639 Backbone Size:0; Vector Backbone:pBAD24; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:11:16 0
pET21a.Tat(48-57)-I-SceI
 
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RRID:Addgene_165041 I-SceI Saccharomyces cerevisiae Ampicillin PMID:33589718 Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:27 0
pMEL15_MATz
 
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RRID:Addgene_166099 MAT Z1 Saccharomyces cerevisiae Ampicillin Addgene QC identified differences to the theoretical sequence that the depositing lab does not believe are of functional concern. Vector Backbone:p426-SNR52p-gRNA.CAN1.Y-SUP4t, Daran Lab; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_GAL2
 
Resource Report
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RRID:Addgene_166088 GAL2 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXII:291472..291491 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_CUP1-1
 
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RRID:Addgene_166086 CUP1-1 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrVIII:212613..212632 (- strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0
pNSU299_MET16
 
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RRID:Addgene_166091 MET16 Saccharomyces cerevisiae Ampicillin gRNA Binding Coordinates: chrXVI:877523..877542 (+ strand) This is a yeast integration plasmid, and should be linearized with BstZ17I (or elsewhere within the TRP1 gene) before transformation for integration into Chromosome V of the yeast genome. Addgene QC identified several discrepancies compared to the theoretical reference. Backbone Marker:Smith JD et al 2016. PMID 26956608; Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:09:34 0

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